5 minutes from ambient to 40°C

10 minutes from 40°C to 20°C

10 fg Anthracene, Ex=250 nm, Em=400 nm

RAMAN single wavelength (H2O) > 500 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime)

RAMAN dual wavelength (H2O) > 300 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime

Range: settable 200 - 700 nm and zero-order

Bandwidth: 20 nm (fixed)

Monochromator: concave holographic grating, F/1.6, blaze: 300 nm

Range: settable 280 - 900 nm and zero-order

Bandwidth: 20 nm (fixed)

Monochromator: concave holographic grating, F/1.6, blaze: 400 nm

Repeatability: ± 0.2 nm

Accuracy: ± 3 nm setting

Settable Flow Range

Set points 0.001 to 10 ml/min, in 0.001 ml/min increments

Flow Range

0.2 to 10.0 ml/min

Flow Precision

°¤ 0.07% RSD, or °¤ 0.02 min SD whatever is greater, based on retention time at constant room temperature

Flow Accuracy

± 1% or 10 µl/min, whatever is greater, pumping degassed H2O at 10 MPa (100 bar)

Pressure Operating Range

Operating range up to 40 MPa (400 bar, 5880 psi) up to 5 ml/min

Operating range up to 20 MPa (200 bar, 2950 psi) up to 10 ml/min

Pressure Pulsation

< 2 % amplitude (typically < 1.0%) or < 0.3 MPa (3 bar, 44 psi), whatever is greater, at 1 ml/min isopropanol, at all pressures > 1 MPa (10 bar, 145 psi)

Compressibility Compensation

User selectable, based on mobile phase compressibility

Recommended pH Range

Gradient Formation

Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve

Delay Volume

Composition Range

Composition Precision

Integrated Degasser

Number of channels: 4

Internal volume per channel: 1.5 ml

Detection Type

5 minutes from ambient to 40°C

10 minutes from 40°C to 20°C

Single wavelength operation:

RAMAN (H2O) > 500 (noise reference measured at signal) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell

RAMAN (H2O) > 3000 (noise reference measured at dark value)

Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell

Dual wavelength operation:

RAMAN (H2O) > 300 Ex 350 nm, Em 397 nm and Ex 350 nm, Em 450 nm, standard flow cell

Range: settable 200 - 1200 nm and zero-order

Bandwidth: 20 nm (fixed)

Monochromator: concave holographic grating, F/1.6, blaze: 300 nm

Range: settable 200 - 1200 nm and zero-order

Bandwidth: 20 nm (fixed)

Monochromator: concave holographic grating, F/1.6, blaze: 400 nm

Repeatability: ± 0.2 nm

Accuracy: ± 3 nm setting

Delay Volume (in ul)

Fraction collector inlet to diverter valve: ~50 (typical, depends on length of the tubing)

Diverter valve: ~15

Diverter valve to needle: ~10

Needle: ~4

~20 mL with 48 mm tubes

~30 mL with 75 mm tubes

Time slices

Peak (threshold, up- / downslope)

Timetable (combination of time intervals and peak)

Manual trigger (supported only with Agilent Instant Pilot G4208A)

Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position

Continuous flow: optional, available only when using deep well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste

Needle into location: Needle pushes into the vessel as deep as specified, for the use with capped vials and test tubes and well plates with closing mats

Droplet setup mode: The tip of the fraction collector needle will initially move down to the bottom of the well. Then it will slowly move upwards while the fraction is collected. The droplet setup mode enables the fraction collector to collect small fractions without bubbles

4 x well-plates full tray (MTP)*

2 x well-plates std. tray + 10 funnels with external containers* (+ 1 half tray)

2 × well-plates std. tray (MTP) + 10 × 2 ml vials* (+ 1 half tray)

100 x 2 ml in std. tray (+ 1 half tray)*

3 x 40 x 2 ml in half tray*

3 x 40 funnels in half tray

3 x 15 x 6 ml in half tray*

Full tray with 40 test tubes (30 mm OD, max. height 48 mm, ~20 ml vol.)

Full tray with 60 test tubes (25 mm OD, max. height 48 mm

Full tray with 126 test tubes (16 mm OD, max. height 48 mm)

Full tray with 215 test tubes (12 mm OD, max. height 48 mm)

48 mm with long needle assembly G1367-87200

75 mm with short needle assembly G1364-87202

Detection Type

< ± 0.7 x 10 ^-5 AU at 254 nm and 750 nm

Early maintenance feedback (EMF), electronic records of maintenance and errors

Injection Range

0.1 – 100 µL in 0.1 µL increments (recommended 1 µl increments)

Up to 40 µL with reduced injection volume kit (hardware modification required)

Up to 1500 µl with multiple draw (hardware modification required)

Precision

< 0.25 % from 5 – 40 µL

< 0.5 % from 2 – 5 µL

< 0.7 % from 1 – 2 µL

< 1.5 % from 0.5 – 1 µL

Carryover

Typically < 0.004%

Sample Viscosity Range

0.2 – 5 cp

Sample Capacity

2 x well plates (MTP) + 10 x 2 ml vials

108 x 2 ml vials in 2 x 54 vial plate plus 10 additional 2 ml vials

30 x 6 ml vials in 2 x 15 vial plate

100 Micro vial tray

54 Eppendorf tubes (0.5/1.5/2 ml) in 2 x 27 Eppendorf tube plate

Max-Light Cartridge Cell (10 mm, V [Æ’], 1.0ul), 60 bar (870 psi) pressure maximum Max-Light Cartridge Cell (60 mm, V [Æ’], 4.0ul), 60 bar (870 psi) pressure maximum Max-Light Cartridge Test Cell

Operating range up to 60 MPa (600 bar, 8700 psi) up to 5 ml/min

Operating range up to 20 MPa (200 bar, 2950 psi) up to 10 ml/min

Number of channels: 4

Internal volume per channel: 1.5 ml

0.05 - 5.0 ml/min

Up to 60 MPa (600 bar, 8700 psi) up to 5 ml/min

Number of channels: 2

Internal volume per channel: 0.45 ml

< ± 0.25 x 10^-5 AU, at 230 nm (single wavelength detection)

< ± 0.80 x 10^-5 AU, at 230 nm and 254 nm (dual wavelength detection)

Detection Type

< ± 3 x 10 ^-6 AU at 230/4 nm, slit width 4 nm, TC 2 s, ASTM

> 2 AU (5%) at 265 nm

Typically 2.5 AU (5%)

Detection Type

< ± 0.7 x 10 ^-5 AU at 254 nm and 750 nm

Detection Type

< ± 0.7 x 10^-5 AU, at 254 and 750 nm