GE AKTA Avant 150 FPLC System
Flow Rate Range
0.01 to 150 ml/min (up to 300 ml/min during column packing)
Maximum Operating Pressure
5 MPa (725 psi)
Wavelength Range
190 to 700 nm in steps of 1 nm, up to 3 wavelengths
- Designed for preparative purification of proteins and other biomolecules
- Integrated P9H-S Sample Pump for automated, large-volume sample applications
- Integrated valves, detectors, and fraction collection support automated purification workflows
- Built-in fraction collector with cooling helps protect purified samples and supports unattended overnight runs
- Modular design allows expansion with additional sample, buffer, outlet, column, and optional component modules
- Integrated instrument display provides convenient monitoring of system status and method progress
- UNICORN software provides centralized instrument control, experimental planning, run supervision, and data evaluation
- BufferPro enables automated buffer preparation and facilitates screening of optimal buffer compositions
- Integrated Design of Experiments (DoE) tools support structured method development and optimization
- Predefined methods and graphical method phases simplify creation and modification of purification protocols
- Column run-history tracking records usage and cleaning history to support column management
The GE ÄKTA avant Preparative Liquid Chromatography System is designed for the purification of proteins and other biomolecules, providing an integrated platform for chromatography method and process development. The system incorporates the valves, detectors, and fraction collection capabilities needed for automated purification, allowing researchers to screen columns, chromatography media, buffers, and operating parameters when developing and optimizing purification methods.
Designed for flexible laboratory workflows, the ÄKTA avant features a modular architecture that can be expanded with additional modules for samples, buffers, outlets, columns, and other optional components. A built-in fraction collector with cooling functionality helps protect purified samples and supports unattended overnight runs, while the integrated instrument display provides convenient monitoring of system status and method progress. BufferPro functionality further streamlines workflows through automated buffer preparation and screening of buffer compositions.
The system is controlled through UNICORN software, providing comprehensive instrument control, experimental planning, run supervision, and data evaluation. Predefined methods and graphical method phases simplify protocol development and editing, while column run-history tracking supports ongoing column management. Integrated Design of Experiments (DoE) functionality provides a structured approach to method optimization, helping laboratories efficiently develop and refine reproducible purification processes.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The GE AKTA Avant 150 FPLC System includes:
- P9H A System Pump A
- P9H B System Pump B
- P9H-S Sample Pump
- U9-M UV Monitor
- U9-D UV Detector
- V9H-IS Sample Inlet Valve
- C9 Conductivity Monitor
- V9H-PH Valve
- V9H-C Column Valve
- V9H-Inj Injection Valve
- V9H-O Outlet Valve
- V9H-IA Inlet Valve
- V9H-IB Inlet Valve
- M9 Mixer w/ 5ml Chamber
- Built-In Fraction Collector
- 6 x Fraction Collector Cassettes
- Windows 11 Professional 64-Bit Data Station with Installed UNICORN Version 7.6 Software
- Installation Calibration Report
- Communication Cables
- Tubing, Power Cords
- Full 180 Day Parts and Labor Warranty
GE AKTA Avant 150 FPLC System Specifications
|
Flow Rate Range |
0.01 to 150 ml/min (up to 300 ml/min during column packing) |
|
Flow Rate Accuracy |
± 1.5% |
|
Flow Rate Precision |
RSD <0.5% |
|
Pressure Range |
0 to 5 MPa (725 psi) |
|
Viscosity Range |
0.35 to 5 cP |
|
Wavelength Range |
190 to 700 nm in steps of 1 nm, up to 3 wavelengths |
|
Absorbance Range |
-6 to 6 AU |
|
Resolution |
0.001 mAU |
|
Linearity |
Within ± 2% at 0 to 2 AU |
|
Drift |
≤ | 0.2 mAU | AU/h (2 mm cell at 280 nm) |
|
Noise |
< 0.08 mAU |
|
Operating Pressure |
0 to 2 MPa |
|
Lamp Operating Time |
> 5000 hours |
|
Flow Cell |
Optical path length: 2 mm Cell volume: 2 ul Total volume: 11 ul |
|
Conductivity Range |
0.01 to 999.9 mS/cm |
|
Accuracy |
± 0.01 mS/cm or ± 2%, whichever is greater (within 0.3 to 300 mS/cm) |
|
Operating Pressure |
0 to 5 MPa |
|
Flow Cell Volume |
22 ul |
|
Temperature Monitor Range |
0°C to 99°C |
|
Temperature Monitor Accuracy |
± 1.5°C within 4°C to 45°C |
|
pH Reading Range |
0 to 14 |
|
Accuracy |
± 0.1 pH unit within pH 2 to 12, temperature within ± 3°C from calibration temperature |
|
Operating Pressure |
0 to 0.5 MPa |
|
Flow Cell Volume |
129 ul |
|
Number of Fractions |
Up to 576 |
|
Vessel Types |
Deep well plates, 96, 48, or 24 wells |
|
Fraction Volumes |
0.1 to 250 ml
|
|
Spillage-Free Mode |
Automatic or Accumulator |
|
Flammable Liquids |
No |
|
Organic Solvents |
No |
|
Delay Volume with pH Electrode and flow restrictor off line (UV-Dispenser Head) |
1807 ul |
|
Dimensions (L x D x H) |
33.9 x 28 x 26 in. (86 x 71 x 66 cm) |
|
Weight |
255.7 lb. (116 kg) |
Computer Specifications |
|
| Processor | Intel Core I5-8500 CPU |
| RAM | 32.0 GB |
| Hard Drive | 500GB Internal SSD |
| Operating System | Windows 11 Professional, 64-bit |
- Inspect all displays, replacing any dim or dead pixels to ensure optimal visual feedback
- Verify connections of all components via Uni-Net 1 & 2 interfaces for secure and reliable communication
- Check Uni-Net cables, replacing any faulty ones to ensure uninterrupted data transmission
- Replace all PEEK tubing with new OEM tubing and inspect all components within the flow path for potential clogs
- Confirm successful software interfacing with the FPLC system for seamless operation
- Replace pump piston seals, wash seals, and seal wash tubing, performing ultrasonic cleaning on each check valve in the pump flow path
- Test UV lamp and confirm full functionality, replace if needed
- Install a new pH probe and verify the conductivity cell for accurate readings
- Calculate and adjust delay volume in software to reflect system configuration
- Verify fraction collector alignment and calibration with 15mL, 50mL, and microplate receptacles to ensure accuracy
- Run installation tests and thoroughly document all findings for comprehensive record-keeping
Technician Inspection and Recertification Checklist: FPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment| Task | Completed? | Remarks? |
| Inspect all displays and replace dim/dead pixels | Completed | |
| Verify connections via Uni-Net 1 & 2 interfaces | Completed | |
| Check and replace any malfunctioning Uni-Net cables | Completed | |
| Replace PEEK tubing with OEM tubing and check for clogs | Completed | |
| Confirm software interfacing with FPLC system | Completed | |
| Replace pump seals and wash tubing; clean check valves | Completed | |
| Test UV lamp and confirm full functionality, replace if needed | Completed | |
| Install new pH probe and verify conductivity cell | Completed | |
| Calculate delay volume and update in software | Completed | |
| Verify fraction collector alignment with 15mL, 50mL, and microplate receptacles | Completed | |
| Run installation tests and document all findings | Completed |
102 Tide Mill Road, Hampton NH 03842
customerservice@marshallscientific.com | (603) 601-8511 |
www.marshallscientific.com
Copyright © 2024 by Marshall Scientific LLC. All rights reserved.
Downloads
Click Here to View the Technical Specs for the GE AKTA Avant 150 FPLC System
