BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader
Detection Methods
FL, TRF, FP, Lum., UV-Vis Abs., AlphaScreen/AlphaLISA
Temperature Range
Ambient +4°C to 65°C
Read Methods
End point, kinetic, spectral scanning, well area scanning
- Compact, advanced multi-detection system with patented Hybrid Technology combines monochromator-based and filter-based fluorescence detection.
- Quadruple mono system with two double-grating monochromators for superior spectral scanning.
- Variable bandpass selection enhances performance.
- Filter/mirror system optimizes energy delivery, ensuring high signal-to-noise ratios and faster read speeds.
- Compatible with Take3 Plate for low-volume nucleic acid quantification.
- Ideal for precise and efficient results in various biochemical assays.
- Offers unparalleled sensitivity and versatility for various microplate-based assays.
- Broad spectrum of detection modes: Fluorescence Intensity, Time-Resolved Fluorescence, Luminescence, UV-Visible Absorbance, and more.
The BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader is a compact, advanced multi-detection system built with patented Hybrid Technology, melding monochromator-based and filter-based fluorescence detection systems. This unique combination offers unparalleled sensitivity and versatility, accommodating a wide array of microplate-based assays both for present and future requirements. Key features include a broad spectrum of detection modes such as Fluorescence Intensity, Time-Resolved Fluorescence, Luminescence, UV-Visible Absorbance, and more. Additionally, its modular and upgradeable architecture allows for cost-effective customization, ensuring it meets evolving laboratory needs.
At its core, the Synergy H4 houses a quadruple mono system with two double-grating monochromators, facilitating superior spectral scanning performance and flexibility. The variable bandpass selection further enhances the system's performance. On the other hand, the filter/mirror system of the Synergy H4 optimizes energy delivery to the sample, ensuring high signal-to-noise ratios, faster read speeds, and precise control over optical parameters. The unit's compatibility with the Take3 Plate also enables low-volume nucleic acid quantification, making the Synergy H4 an invaluable tool for achieving precise and efficient results in a variety of biochemical assays.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader includes:
- Windows 11 Professional 64-Bit Data Station with Pre-Loaded Gen5 PC Software
- 460 nm, 528 nm, 590 nm, and 645 nm Emission Filters
- 340 nm, 460 nm, 485 nm, and 540 nm Excitation Filters
- ABS, FLD, LUM, and Self Test Calibration Report
- Interface Cable
- Operator's Manual
- Full 180 Day Part and Labor Warranty
BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader Specifications
|
Absorbance Specifications |
|
|
Light Source |
Xenon flash lamp |
|
Wavelength Selection |
Monochromator |
|
Wavelength Range |
230 to 999 nm, in 1 nm increments |
|
Bandpass |
2 nm (230 to 285 nm), 4 nm (> 285 nm) |
|
Dynamic Range |
0 to 4.0 OD |
|
Photometric Resolution |
0.0001 OD |
|
Pathlength Correction |
Yes |
|
Photometric Accuracy |
< 1% at 2.0 OD typical < 3% at 3.0 OD typical |
|
Photometric Repeatability |
< 0.5% at 2.0 OD typical |
|
Fluorescence Intensity Specifications |
|
|
Light Source |
Tungsten halogen lamp |
|
Wavelength Selection |
Double grating monochromators (top/bottom) and deep blocking filters/dichroic mirrors (top) |
|
Wavelength Range |
Monochromators: 250 to 850 nm Filters: 200 to 700 nm (850 nm option) |
|
Dynamic Range |
Monochromators: 5 decades Filters/Mirrors: > 6 decades |
|
Sensitivity |
Top Monochromator: Fluorescein 2 pM typical (0.2 fmol/well 384-well plate) Bottom Monochromator: Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) Filters/Mirrors: Fluorescein 1 pM typical (0.1 fmol/well 384-well plate) |
|
Luminescence Specifications |
|
|
Wavelength Range |
300 to 700 nm |
|
Dynamic Range |
< 6 decades |
|
Sensitivity |
10 amol ATP typical (flash) |
|
Fluorescence Polarization Specifications |
|
|
Light Source |
Tungsten halogen |
|
Wavelength Selection |
Deep blocking filters/dichroic mirrors (top) |
|
Wavelength Range |
400 to 700 nm (320 to 850 nm option) |
| Sensitivity |
3 mP at 1 nm fluorescein typical |
|
Time-Resolved Fluorescence Specifications |
|
|
Light Source |
High energy xenon flash lamp |
|
Wavelength Range |
200 to 700 nm (850 nm option) |
| Sensitivity |
Europium 60 fM typical with filters (6 amol/well in 384-well plate) |
|
Reagent Dispenser Specifications |
|
|
Number |
2 syringe pumps |
|
Dispense Volume |
5 to 1000 ul in 1 ul increments |
| Minimum Prime Volume |
1.1 mL, 100 ul with back flush |
|
General Specifications |
|
|
Microplate Formats |
Monochromator System: 1 to 384-wells Filter System: 1 to 1536-wells PCR plates, and Take3 Micro-Volume Plates |
|
Read Methods |
End point, kinetic, spectral scanning, well area scanning |
|
Detection Methods |
FL, TRF, FP, Lum., UV-Vis Abs., AlphaScreen/AlphaLISA |
|
Plate Shaking |
Yes |
|
Temperature Control |
Ambient +4°C to 65°C, ± 0.5°C |
|
Dimensions (W x D x H) |
17 x 20.9 x 15 in. (43.5 x 53.1 x 38.1 cm) |
|
Weight |
78 lb. (35 kg.) |
|
Electrical Requirements |
100-240V, 50/60Hz |
|
Computer Specifications |
|
| Processor | Intel Core I5-8350U CPU |
| RAM | 16.0 GB |
| Hard Drive | 512GB Internal SSD |
| Operating System | Windows 11 Professional, 64-bit |
- Confirm the power supply and PCB output correct, stable voltages across all required connections
- Inspect the display for any dim or dead pixels; replace affected components to maintain full visual clarity and operational accuracy
- Verify full functionality of all buttons, ensuring precise and responsive input control
- Check the XY assembly for proper movement and alignment; thoroughly clean all railings, removing any debris or contaminants
- Confirm stable software communication and connection between the instrument and PC, addressing any connectivity issues
- Inspect and clean or replace all filters as required to ensure optimal airflow and performance
- Examine internal wiring and electrical connections for any breaks, disconnections, or tears; replace or secure any compromised wiring
- Test the lamp’s full functionality, replacing it if any performance issues, dimming, or faults are detected
- Execute the necessary validation protocol, documenting completion with a date on a calibration/verification certificate
- Perform and verify appropriate updates to the PC operating system for compatibility and security
- Conduct a thorough cosmetic inspection, ensuring all external components meet quality and aesthetic standards
Technician Inspection and Recertification Checklist: Instruments
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment| Task | Completed? | Remarks? |
| Confirm stable, correct voltage outputs from power supply and PCB | Completed | |
| Inspect display for dim or dead pixels; replace as needed | Completed | |
| Verify functionality of all buttons for precise, responsive control | Completed | |
| Check XY assembly for proper movement; clean railings to remove debris | Completed | |
| Confirm stable software communication between instrument and PC | Completed | |
| Inspect and clean or replace filters to maintain optimal performance | Completed | |
| Examine internal wiring for damage; replace or secure compromised connections | Completed | |
| Test lamp functionality; replace if dimming or faults are detected | Completed | |
| Execute validation protocol and document on calibration/verification certificate | Completed | |
| Update PC operating system for compatibility and security | Completed | |
| Conduct cosmetic inspection to ensure quality of external components | Completed |
102 Tide Mill Road, Hampton NH 03842
customerservice@marshallscientific.com | (603) 601-8511 |
www.marshallscientific.com
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