Biotek ELx405V Microplate Washer
Microplate Types
96- and 384-well
Manifold Type
96-Tube Dual-Action manifold for 96- and 384-well washing
Wash Speed
96-wells, 300 uL/well, 3 cycles: <30 seconds; 384-wells, 100 uL/well, 3 cycles: <80 seconds
- Automatic buffer switching supports selection from up to four wash buffers or reagents
- Programmable shaking and soaking provide added flexibility when configuring wash protocols
- Adjustable dispense volumes in 1 µL increments support precise protocol customization
- Onboard software allows users to create, edit, store, and run multiple protocols
- High-to-low adjustable flow rates accommodate a variety of washing requirements
- Optimized fluid delivery supports gentle processing for sensitive cell-based assays
- Low residual volumes help promote efficient aspiration and consistent assay performance

The BioTek ELx405V Microplate Washer is a versatile automated plate washing system designed to support efficient processing of both 96- and 384-well microplates, the ELx405V provides flexibility for a broad range of microplate-based laboratory workflows. A 96-tube Dual-Action manifold supports both 96- and 384-well washing, while programmable shaking and soaking functions provide additional control over wash protocols. The ELx405V features integrated buffer switching, allowing automated selection from up to four buffer or reagent sources. Programmable wash cycles and adjustable dispense volumes enable protocols to be configured around specific assay requirements, while onboard software allows users to create, edit, and run multiple protocols directly from the instrument.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The Biotek ELx405V Microplate Washer includes:
- (2) 4 Liter Waste Bottles
- (1) 4 Liter Buffer Bottle
- Vacuum Pump
- Vacuum Tubing with Connections
- Power Cord
- Operator's Manual
- Full 180 Day Parts and Labor Warranty
Biotek ELx405V Microplate Washer Specifications
| Microplate Types | 96- and 384-well |
| Onboard Software | Create, edit and run multiple protocols |
| Manifold Types | 96-Tube Dual-Action manifold for 96- and 384-well washing |
| Volume Range | 50 to 3,000 uL/well, in 1 uL increments |
| Wash Cycles | 1-10 |
| Buffer/Reagent Selection | Auto switching module for up to 4 buffers (optional) |
| Supply Bottle | 4 L or 10 L (optional) |
| Dispense Precision | ≤3% CV: 300 uL/well (96-well washing) |
| Residual Volume | <2 uL/well (standard and deep well 96-well plates) |
| Wash Speed | 96-wells, 300 uL/well, 3 cycles: <30 seconds; 384-wells, 100 uL/well, 3 cycles: <80 seconds |
| Flow Rates | High flow to low flow; Optimized rates for cell assays |
| Sterilization | Chemical |
| Dimensions (W x D x H) | 14 x 17 x 11 in. (35.6 x 43.2 x 27.9 cm) |
| Weight | 30 lb. (13.5 kg) |
| Electrical Requirements | 100-240V, 50/60Hz |
- Install new waste and buffer bottles to ensure proper fluid containment and system functionality.
- Integrate a new pump into the system, verifying compatibility and secure connections.
- Verify that the power supply and PCB output the correct voltages for reliable component operation.
- Conduct a self-test on the microplate washer, evaluating all operational parameters.
- Inspect the X-axis motor belt for signs of wear, such as tears, frays, or breakages, replacing if necessary.
- Examine dispense and aspirate tubes for any damage that could affect fluid flow accuracy.
- Confirm internal pump functionality, replacing seals as needed to maintain optimal performance.
- Inspect all internal wiring for frays, tears, or breakages, addressing any faults for electrical safety.
- Verify functionality of all internal and external sensors, ensuring accurate readings across the unit.
- Disinfect the unit using an appropriate disinfection cycle to ensure sterility and safety.
- Check that residual aspiration levels are within the manufacturer’s specified limits for accuracy.
- Conduct a final cosmetic inspection to verify that all external surfaces meet appearance standards.
Technician Inspection and Recertification Checklist: Microplate Washer
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment| Task | Completed? | Remarks? |
| Install new waste and buffer bottles | Completed | |
| Integrate new pump into system, verifying secure connections | Completed | |
| Verify power supply and PCB output correct voltages | Completed | |
| Conduct microplate washer self-test and evaluate results | Completed | |
| Inspect X-axis motor belt for wear, replacing if necessary | Completed | |
| Examine dispense and aspirate tubes for any damage | Completed | |
| Confirm internal pump functionality, replace seals as needed | Completed | |
| Inspect internal wiring for frays, tears, or breakages | Completed | |
| Verify functionality of all internal and external sensors | Completed | |
| Disinfect unit with appropriate disinfection cycle | Completed | |
| Check residual aspiration levels meet specifications | Completed | |
| Conduct cosmetic inspection for appearance standards | Completed |
Downloads



Click Here to View the Technical Specs for the Biotek ELx405V Microplate Washer