Biorad CFX Connect Real-Time PCR System
Block Configuration
96-Well
Maximum Ramp Rate
5°C/second
Temperature Uniformity
± 0.4°C
Features
- Dual-target detection system with precise, reproducible thermal cycling
- Thermal gradient feature allows efficient assay optimization in a single run
- Factory-calibrated dyes enable fast startup and accurate fluorescence detection
- Integrated with CFX Maestro Software for experiment design and data analysis
- Advanced statistical tools include t-tests, ANOVA, and custom visualizations
- Supports MIQE-compliant RDML output via qbase+ Software integration
- Compact and user-friendly design ideal for research and diagnostic labs
The Bio-Rad CFX Connect Real-Time PCR Detection System offers reliable two-target detection with the same trusted thermal performance of the CFX96 Touch. Designed for 96-well plate formats, it features a robust thermal cycler engineered for consistent heating across all wells, enabling reproducible results run after run. A built-in thermal gradient simplifies assay optimization, allowing users to identify ideal annealing conditions quickly and efficiently within a single experiment. This system is ideal for laboratories seeking high-quality results with a streamlined, user-friendly workflow.
CFX Connect incorporates solid-state optical technology for accurate, sensitive fluorescence detection. Its dual-target capability and scanning optics enable flexible multiplexing and precise quantification. Combined with factory-calibrated dyes and intuitive setup, the system is ready to deliver high-quality results with minimal startup time, supporting a broad range of research and diagnostic workflows. With LED excitation and filtered photodiode detection, the system ensures robust and reliable signal clarity.
Control and analysis are handled through CFX Maestro Software, a comprehensive platform for experiment design, data acquisition, and statistical evaluation. From automated t-tests and ANOVA to custom data views and high-resolution charting, CFX Maestro streamlines complex workflows with ease. For researchers requiring MIQE-compliant output, integration with qbase+ Software enables RDML file export and supports efficient submission for publication. The software’s flexible interface also supports multiple plate formats and experimental setups, enhancing its utility across varying protocols. Please Note: We recommend performing a Dye Calibration for the Dye you plan on using for your application(s). Please refer to the Operator's Manual for instructions.
All of our equipment is fully tested to perform at factory specifications before being shipped.
INCLUDED:
The Biorad CFX Connect Real-Time PCR System includes:
The Biorad CFX Connect Real-Time PCR System includes:
- Biorad CFX96 Qualification Plate Calibration Report
- Windows 11 Professional 64-Bit Data Station
- Power Cord
- Operator's Manual
- Full 180 Day Parts and Labor Warranty
Biorad CFX Connect Real-Time PCR System Specifications
| Block Configuration | 96-Well (1 to 50 uL reactions, recommended for 10 to 25 uL reactions) |
| Temperature Range | 0°C to 100°C |
| Temperature Uniformity | ± 0.4°C well-to-well within 10 seconds of arrival at 90°C |
| Temperature Accuracy | ± 0.2°C of programmed target at 90°C |
| Maximum Ramp Rate | 5°C/second |
| Average Ramp Rate | 3.3°C/second |
| Gradient Operational Range | 30°C to 100°C |
| Gradient Programmable Span | 1°C to 24°C |
| Scan Time | 12 seconds (all channels), 3 seconds (FAM only) |
| Excitation Source | 3 filtered LEDs |
| Detection Channels | 3 filtered photodiodes |
| Range of Excitation/Emission Wavelengths | 450 to 580 nm |
| Dynamic Range | 10 orders of magnitude |
| Sensitivity | Detects 1 copy of target sequence in human genomic DNA |
| Multiplexing | Up to 2 targets per well |
| Dimensions (W x D x H) | 13 x 18 x 14 in. (33 x 46 x 36 cm) |
| Weight | 47 lb. (21 kg) |
| Electrical Requirements | 100-240V, 50/60Hz |
|
Computer Specifications |
|
| Processor | Intel Core I5-8350U CPU |
| RAM | 16.0 GB |
| Hard Drive | 512GB Internal SSD |
| Operating System | Windows 11 Professional, 64-bit |
Thermal Cycler Inspection and Recertification Checklist
- Validate that the power supply and PCB consistently deliver accurate, stable voltages across all circuits and connections, confirming operational reliability
- Thoroughly inspect the display for any dim, flickering, or dead pixels, replacing compromised segments to ensure clear visibility and precise data display
- Verify complete functionality and responsiveness of all buttons and knobs, confirming accurate and reliable input across all controls
- Ensure the machine completes all internal diagnostic checks with zero errors, indicating system readiness and functional integrity
- Confirm the machine accurately identifies attached blocks (96-well, dual 48, etc.), with seamless recognition across different configurations
- Conduct a detailed inspection of internal wiring and all electrical connections, checking for any signs of breaks, disconnections, or tears; replace or repair any underperforming components
- Examine the connector between the block and base for bent or broken pins, replacing as needed to maintain secure, reliable connections
- Calibrate well temperatures with a NIST-traceable thermometer, verifying accuracy and uniformity across all wells to meet specification standards
- Perform a comprehensive cosmetic inspection, ensuring all exterior surfaces and components are free of damage, wear, or defects, maintaining aesthetic and professional quality
Technician Inspection and Recertification Checklist: Thermal Cycler
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment| Task | Completed? | Remarks? |
| Validate stable, accurate voltage outputs from power supply and PCB across circuits | Completed | |
| Inspect display for dim, flickering, or dead pixels; replace as needed for clarity | Completed | |
| Verify functionality and responsiveness of all buttons and knobs | Completed | |
| Confirm machine completes internal diagnostics with zero errors | Completed | |
| Ensure accurate block identification across configurations (96-well, dual 48, etc.) | Completed | |
| Inspect internal wiring and connections for integrity; repair or replace as needed | Completed | |
| Check connector between block and base for bent or broken pins; replace as necessary | Completed | |
| Calibrate well temperatures with a NIST-traceable thermometer for accuracy | Completed | |
| Conduct cosmetic inspection to ensure quality and professionalism of all surfaces | Completed |
102 Tide Mill Road, Hampton NH 03842
customerservice@marshallscientific.com | (603) 601-8511 |
www.marshallscientific.com
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