Standard: 13 µL volume, 10 mm cell path length and 120 bar (1740 psi) pressure maximum
FLD Detector
Specification
Detection Type
Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis
Performance Specifications
Single wavelength operation: ‚ RAMAN (H2O) > 500 (noise reference measured at signal) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell ‚ RAMAN (H2O) > 3000 (noise reference measured at dark value) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell Dual wavelength operation: RAMAN (H2O) > 300 Ex 350 nm, Em 397 nm and Ex 350 nm, Em 450 nm, standard flow cell.
Light source
Xenon Flash Lamp, normal mode 20 W, economy mode 5 W, lifetime 4000 h
Pulse frequency
296 Hz for single signal mode 74 Hz for economy mode
Excitation Monochromator
Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 300 nm
Emission Monochromator
Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 400 nm
Reference System
In-line excitation measurement
Timetable programing
up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters
Spectrum acquisition
Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200 ‚ 400 nm, 10 nm step) Step size: 1 ‚ 20 nm Spectra storage: All
Wavelength characteristic
Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting
Flow cells
Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz
< ± 0.7 x 10^-5 AU, at 254 and 750 nm
With 10 mm Max-Light cartridge cell: < ± 3 x 10-6 AU at 230/4 nm, slit width 4 nm, TC 2 s , ASTM
With 60 mm Max-Light cartridge cell (included): < ± 0.6 x 10-6 AU at 230/4 nm, slit width 4 nm, TC 2 s , ASTM
Deuterium: 190 nm to approximately 800 nm
Tungsten: 370 nm to 1100 nm
Within ± 0.005 AU at 440, 465, 546.1, 590, and 635 nm at approximately 1 AU using the NIST-traceable 930e
± 0.01 AU at 235, 257, 313, 350 nm using a 6 % w/v potassium dichromate NIST-traceable 935a solution in 0.01 N sulfuric acid
> 0.03% at 340 nm measured with 50 g/l NaNO2 solution
> 0.05% at 220 nm measured with 10 g/l NaI solution
> 1% at 198 nm measured with 1.2% KCl solution
Analysis Run Time
30 to 45 min
Number of Samples
Up to 12 samples/chips
Sample Volume
1 µl
Sizing Range
50 to 7000 bp
Ambient Operating Temperature
5° to 40°C (41° to 104°F)
Electrical Requirements
100-240VAC, 50-60Hz
Power Consumption
60 VA
Dimensions (W x D x H)
162 x 412 x 290 mm (6.4 x 16.2 x 11.4 in)
Safety Standards: IEC, CSA, UL
Installation Category II, Pollution Degree 2
Standard: 14 µL volume, 10 mm cell path length and 40 bar (580 psi) pressure maximum
Recorder/integrator: 100 mV or 1 V, output range 0.001 - 2 AU, one output
Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN
Extensive diagnostics, error detection, and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter.
Delay Volume (in ul)
Fraction collector inlet to diverter valve: ~500 (typical, depends on length of the tubing)
Diverter valve: ~15
Diverter valve to needle: ~110
Needle: ~5
Time slices
Peak (threshold, up- / downslope)
Timetable (combination of time intervals and peak)
Manual trigger (supported only with Agilent Instant Pilot G4208A)
Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position
Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste