Standard: 13 µL volume, 10 mm cell path length and 120 bar (1740 psi) pressure maximum
FLD Detector
Specification
Detection Type
Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis
Performance Specifications
Single wavelength operation: ‚ RAMAN (H2O) > 500 (noise reference measured at signal) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell ‚ RAMAN (H2O) > 3000 (noise reference measured at dark value) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell Dual wavelength operation: RAMAN (H2O) > 300 Ex 350 nm, Em 397 nm and Ex 350 nm, Em 450 nm, standard flow cell.
Light source
Xenon Flash Lamp, normal mode 20 W, economy mode 5 W, lifetime 4000 h
Pulse frequency
296 Hz for single signal mode 74 Hz for economy mode
Excitation Monochromator
Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 300 nm
Emission Monochromator
Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 400 nm
Reference System
In-line excitation measurement
Timetable programing
up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters
Spectrum acquisition
Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200 ‚ 400 nm, 10 nm step) Step size: 1 ‚ 20 nm Spectra storage: All
Wavelength characteristic
Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting
Flow cells
Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz
< ± 0.7 x 10^-5 AU, at 254 and 750 nm
With 10 mm Max-Light cartridge cell: < ± 3 x 10-6 AU at 230/4 nm, slit width 4 nm, TC 2 s , ASTM
With 60 mm Max-Light cartridge cell (included): < ± 0.6 x 10-6 AU at 230/4 nm, slit width 4 nm, TC 2 s , ASTM
< ± 0.25 x 10^-5 AU, at 230 nm (single wavelength detection)
< ± 0.80 x 10^-5 AU, at 230 nm and 254 nm (dual wavelength detection)
Standard: 14 µL volume, 10 mm cell path length and 40 bar (580 psi) pressure maximum
Recorder/integrator: 100 mV or 1 V, output range 0.001 - 2 AU, one output
Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN
Extensive diagnostics, error detection, and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter.
Delay Volume (in ul)
Fraction collector inlet to diverter valve: ~500 (typical, depends on length of the tubing)
Diverter valve: ~15
Diverter valve to needle: ~110
Needle: ~5
Time slices
Peak (threshold, up- / downslope)
Timetable (combination of time intervals and peak)
Manual trigger (supported only with Agilent Instant Pilot G4208A)
Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position
Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste
Wavelength Characteristic
GLP Features
Early maintenance feedback (EMF), electronic records of maintenance and errors
Communications
Controller-area network (CAN), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
Safety Features
Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
Injection Range
0.1 ‚ 100 µl in 0.1 µl increments (recommended 1 µl increments)
Up to 1500 µl with multiple draw (hardware modification required)
Replicate Injections
1 ‚ 99 from one vial
Precision
Typically < 0.25 % RSD of peak areas from 5 to 100 µl
Typically < 1 % RSD of peak areas from 1 to 5 µl
Minimum Sample Volume
1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial
Carryover
Typically < 0.1 %, < 0.05 % with external needle cleaning
Sample Viscosity Range
0.2 ‚ 50 cp
Metering Devices
Metering pump in high pressure flow path
Sample Capacity
100 ƒ” 2 ml vials in 1 tray
40 ƒ” 2 ml vials in ½ tray
15 ƒ” 6 ml vials in ½ tray (Agilent vials only)
Injection Cycle Time
50 seconds for draw speed 200 µl/min, ejection speed 200 µl/min, injection volume 5 µl
