Microplate Types
ANSI/SBS Standard and low profile 96, 384, and 1536 wells; maximum plate height of 14.6 mm
Microplate Capacity
10-, 30-, and 50-plate stacks are removable and interchangeable; 96-/384-well plates: up to 50 plates; 1536-well plates: up to 75 plates
Processing Speed (Plate Exchange Time)
<33 seconds
Overall Dimensions (W x D)
7 x 18.5 in (18 x 47 cm) height varies depending on connected instruments and stacks used
ANSI/SLAS Standard and low profile 96, 384, and 1536 wells; maximum plate height of 14.6 mm
96-well
Low profile and standard height
Manifold Types
96 Tube Dual Action manifold for 96-well washing
Volume Range
50 to 3000 microliter increments
Wash Speed
96-wells, 300 µL/well, 3 cycles: <30 seconds
Electrical Requirements
100 to 240V, 50-60Hz
96 and 384 well plates
96 Tube Dual Action manifold for 96 and 384 well washing
384-wells,100 µL/well, 3 cycles: <80 seconds
100 - 240 Volts AC. 50/60 Hz
96-/384-well
384-wells, 100 µL/well, 3 cycles: <80 seconds
50 to 2000 microliter increments
96- and 384-well
96 Tube Dual Action manifold for 96- & 384-well washing
Wavelength Range
340 to 900 nm
340, 405, 450, 490, and 630 nm
Wavelength Bandpass
10 nm
Standard 96-well microplates
Incubation
Temperature control up to 50ºC
Temperature uniformity ± 0.5°C at 37°C
Plate Shaking
Low, Medium, High, and Variable Speeds
Read Modes
Endpoint, Kinetic, and Linear Scan
Absorbance Measurement Range
0.000 to 4.000 OD (400 to 900 nm range) 0.000 to 3.000 OD (340 to 400 nm range)
Absorbance Accuracy
± 1.0% ± 0.010 OD from 0.000 to 2.500 OD @ 405 nm
± 1.0% ± 0.010 OD from 0.000 to 2.000 OD @ 340 nm
400 to 750 nm (Filter-Based)
Filters Included
405, 450, 490, and 630 nm
Standard 6-, 12-, 24-, 48-, and 96-well microplates
N/A
Endpoint, Kinetic, and Well Area Scanning
0.000 to 3.000 OD
96-Well Plate, Normal Read Mode:
± 1.0% ± 0.010 OD from 0.000 to 2.000 OD at 405 nm
96-Well Plate, Rapid Read Mode:
± 2.0% ± 0.020 OD from 0.000 to 2.000 OD at 405 nm
25 to 3000 microliter increments
96-wells, 300 ƒšµL/well, 3 cycles: <30 seconds
96 and 384-well
Two manifolds, one with 96 aspirate tubes and the other with 96 dispense tubes
<10 seconds
Overall Dimensions (L x W x H)
21 x 8 x 15 in (54 x 21 x 38 cm)
384-well
Two manifolds, one with 192 aspirate tubes and the other with 192 dispense tubes for processing 384-well plates
384-wells, 100 ƒšµL/well, 3 cycles: <80 seconds
96-well (with dual/96-tube manifold installed)
Two manifolds, one with 96 aspirate tubes and the other with 96 dispense tubes, for processing 96 and 384 well plates
0.000 to 4.000 OD
2.000 to 2.500 OD ± 3.0% ± 0.010 OD Normal and Rapid modes, 96- and 384-well plates0.000 to 1.000 OD ± 1% ± 0.010 OD Sweep mode, 96- and 384-well plates
Temperature control range from Ambient +4°C to 50°C
96-well: 3 cycles, 300 µL/well, 96-tube manifold, final aspirate: °¤ 30 seconds
384-well: 3 cycles, 100 µL/well, 96-tube manifold, final aspirate: °¤ 80 seconds
96-well: 3 cycles, 300 µL/well, 96-tube manifold, final aspirate: ≤ 30 seconds
Absorbance Specifications
Light Source
Xenon flash lamp
Wavelength Selection
Double monochromator
230 to 999 nm, in 1 nm increments
Bandpass
2 nm (230-285 nm), 4 nm (>285 nm)
Pathlength Correction
Yes
Monochromator Wavelength Accuracy
± 2 nm
Monochromator Wavelength Repeatability
± 0.2 nm
Dynamic Range
0 to 4.0 OD
Photometric Resolution
0.0001 OD
Photometric Accuracy
< 1% at 2.0 OD typical
Photometric Linearity
< 1% from 0 to 3.0 OD
Photometric Repeatability
< 0.5% at 2.0 OD
Reading Speed
96 wells: 11 seconds
384 wells: 22 seconds
Fluorescence Intensity Specifications
Detector
Dedicated red shifted PMT
Double grating monochromators (top read only)
250 to 900 nm
5 decades
Sensitivity (Top Read Only)
Fluorescein 2 pM typical (0.2 fmol/well, 384-well plate)
Luminescence Specifications
< 6 decades
Sensitivity
10 amol ATP typical (flash)
Detection System
Low noise PMT
300 to 700 nm
Standard 6-, 12-, 24-, 48-, 96-, 384-well microplates
Max Plate Height:
Luminescence/Fluorescence modes: plates up to 1.25‚ (31.75 mm) high Absorbance mode: plates up to 0.8‚ (20.30 mm) high
Environment
Operational temperature 18º to 40°C
Absorbance: Xe flash light source
Fluorescence: Tungsten quartz halogen
Humidity
10% to 85% relative humidity (non-condensing)
Power Consumption
100 VA max, 130 VA max with injectors
