Wavelength Range
340 to 900 nm
340, 405, 450, 490, and 630 nm
Wavelength Bandpass
10 nm
Standard 96-well microplates
Incubation
Temperature control up to 50ºC
Temperature uniformity ± 0.5°C at 37°C
Plate Shaking
Low, Medium, High, and Variable Speeds
Read Modes
Endpoint, Kinetic, and Linear Scan
Absorbance Measurement Range
0.000 to 4.000 OD (400 to 900 nm range) 0.000 to 3.000 OD (340 to 400 nm range)
Absorbance Accuracy
± 1.0% ± 0.010 OD from 0.000 to 2.500 OD @ 405 nm
± 1.0% ± 0.010 OD from 0.000 to 2.000 OD @ 340 nm
400 to 750 nm (Filter-Based)
Filters Included
405, 450, 490, and 630 nm
Microplate Capacity
Standard 6-, 12-, 24-, 48-, and 96-well microplates
N/A
Endpoint, Kinetic, and Well Area Scanning
0.000 to 3.000 OD
96-Well Plate, Normal Read Mode:
± 1.0% ± 0.010 OD from 0.000 to 2.000 OD at 405 nm
96-Well Plate, Rapid Read Mode:
± 2.0% ± 0.020 OD from 0.000 to 2.000 OD at 405 nm
340‚850 nm
Wavelength Selection
Monochromator, tunable in 1-nm increments
Wavelength Bandwidth
°¤ 2.0 nm full width half maximum
Wavelength Accuracy
± 1.0 nm across wavelength range
Wavelength Repeatability
± 0.2 nm across all optical channels
Photometric Range
0.000 to 4.000 OD
Photometric Resolution
0.001 OD
Photometric Accuracy/Linearity
< ± 1.0% and ± 0.006 OD, 0.0-2.0 OD
Photometric Precision
< ± 1.0% and ± 0.003 OD, 0.0-2.0 OD
Stray Light
< 0.05% @ 340 nm
Light Source
Xenon Flash Lamp
Microplate Read Time ‚ Endpoint
9 seconds
Microplate Read Time ‚ Kinetic
9-second minimum interval
Temperature Range
Ambient + 4° to 45°C
Temperature Uniformity
±0.5 °C @ 37 °C well-to-well
Sample Size
1 microliter
Path Length
1mm (with auto-ranging to 0.2 mm)
Xenon flash lamp
Detector Type
2048-element linear silicon CCD array
220 to 750 nm
1 nm
Wavelength Resolution
3 nm (FWHM at Hg 546 nm)
Absorbance Precision
0.003 absorbance (1mm path)
Absorbance Range
0.02 to 75 (10 mm equivalent absorbance)
Detection Limit
2 ng/microliter (dsDNA)
Maximum Concentration
3700 ng/microliter (dsDNA)
Measurement Cycle Time
10 seconds
Dimensions
14 x 20 cm
2.000 to 2.500 OD ± 3.0% ± 0.010 OD Normal and Rapid modes, 96- and 384-well plates0.000 to 1.000 OD ± 1% ± 0.010 OD Sweep mode, 96- and 384-well plates
Temperature control range from Ambient +4°C to 50°C
Performance Specifications
Wavelength Range:
190 - 850 nm
Monochromator, tunable 1.0 nm increments
2 nm
Wavelength Accuracy:
< ± 2.0 nm
± 0.2 nm
0.0 to 4.0 OD
Photometric Accuracy
< ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
< ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
< 0.05% @ 230 nm
Light Source:
Endpoint:
12 seconds
Kinetic:
9 second minimum interval
Ambient + 4 °C up to 45 °C
Temperature Uniformity:
± 0.5 °C @ 37 °C
Fluorescence Photometric Performance
Dual Monochromators
1nm increments
250-850nm
Bandwidth (Excitation & Emission)
9 nm
Time Resolved Fluorescence
Data Collection
50-1450 µ/sec, 200µ/sec increments
Sensitivity
0.5/ well
Luminescence Performance
360-850nm
10 amol/well Alkaline Phosphate 200 µl/well
General Specifications
Microplate Formats
6-, 12-, 24-, 48-, 96- and 384
Detector
Photomultiplier (R-3896)
Read Time
96 < 15 sec, 384 < 45 sec
Shaker Time
0 to 999 seconds
Temperature Control
Ambient +4°C - 45°C
Electrical Requirements
100-240 VAC, 50/60 Hz
Performance
Wavelength Range Excitation
250 to 850 nm
Wavelength Range Emission
Wavelength Selections
Wavelength
Top Read Sensitivity
3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity
8.0 fmol/well FITC 200 µl in 96 wells
Microplate Read Time
Normal Mode
96 wells in 15 seconds
384 wells in 45 seconds
Dynamic Range
6 orders of magnitude
Ambient + 4° up to 45°C
± 1° at 37°C
Spectral Range
190-1000 nm, tunable in 1 nm increments
190-1000 nm
Monochromator, tunable 1.0nm increments
Speed Read Mode
Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds
< ± 1.0 nm
Photometric Accuracy (plate)
Photometric Accuracy (cuvette)
< ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
Photometric Baseline Flatness
< 0.001 OD
Ambient + 4°C up to 45°C
Temperature Uniformity (plate)
± 0.5 °C @ 37°C
± 2% (at 0.76 absorbance at 257 nm)
0.02 to 300 absorbance (10 mm equivalent)
0.002 absorbance (1 mm path)
Minimum Sample Size
0.5µl
Pathlength
1 mm (auto-ranging to 0.05 mm)
190 to 840 nm
± 1 nm
Spectral Resolution
< 1.8 nm (FWHM @Hg 253.7 nm)
2 ng/µL (dsDNA)
15,000 ng/µL (dsDNA)
Measurement Time
< 5 seconds
Sample Pedestal Material
303 stainless steel and quartz fiber
Software Compatibility
Windows XP and Vista (32 bit), Windows 7 Pro (32 and 64 bit), Windows 8 (32 and 64 bit), and Windows 10 Pro (64-bit)
5.5 x 7.9 in. (14 x 20 cm.)
Weight
4.4 lb. (2.0 kg.)
12 VDC, 12 to 18 W (Max: 30 W)
3% (at 0.74 Abs at 350nm)
Pedestal: 0-300 ABS Cuvette: 0 - 1.5 ABS
Accuracy
0.002
Accuracy (Absorption)
3% at (0.74 at 350nm)
Compatibility
Microsoftž Windowsž XP (32-bit) w/Service Pack (SP) 2 or later, Vistaž (32-bit), Windows 7 (32 bit and 64 bit), Windows 10 (64 bit)
Concentration
2ng/µl-15,000ng/µl (dsDNA) ng/µl
Detection Limits
Pedestal: 2ng/µL (dsDNA), 0.10mg/mL (BSA) Cuvette: 0.4ng/µL (dsDNA), 0.01 mg/mL (BSA)
Detection Range
Pedestal: 2-15,000ng/µL (dsDNA) , 0.10 - 400mg/mL (BSA)
14 x 20cm
Heating Range
37°; ±0.5°C
Lamp
Xenon Flash
<3 sec.
No. of Samples
1
Pathlength (Metric)
10; 5; 2; 1mm
Power Consumption
12VDC, 5W
Sample Volume (Metric)
1000, 0.5-2.0µL
=1.8nm (FWHM at Hg 253.7)
System Requirements
Microsoft Windows 7 Professional (32-bit and 64-bit), Windows 8 (32- and 64-bit), and Windows 10 Pro (64-bit)
±1nm
190-840nm
Absorbance Specifications
Double monochromator
230 to 999 nm, in 1 nm increments
Bandpass
2 nm (230-285 nm), 4 nm (>285 nm)
Pathlength Correction
Yes
Monochromator Wavelength Accuracy
± 2 nm
Monochromator Wavelength Repeatability
0 to 4.0 OD
0.0001 OD
< 1% at 2.0 OD typical
Photometric Linearity
< 1% from 0 to 3.0 OD
Photometric Repeatability
< 0.5% at 2.0 OD
Reading Speed
96 wells: 11 seconds
384 wells: 22 seconds
Fluorescence Intensity Specifications
Dedicated red shifted PMT
Double grating monochromators (top read only)
250 to 900 nm
5 decades
Sensitivity (Top Read Only)
Fluorescein 2 pM typical (0.2 fmol/well, 384-well plate)
Luminescence Specifications
< 6 decades
10 amol ATP typical (flash)
Detection System
Low noise PMT
300 to 700 nm
Standard 6-, 12-, 24-, 48-, 96-, 384-well microplates
Max Plate Height:
Luminescence/Fluorescence modes: plates up to 1.25‚ (31.75 mm) high Absorbance mode: plates up to 0.8‚ (20.30 mm) high
Environment
Operational temperature 18º to 40°C
Absorbance: Xe flash light source
Fluorescence: Tungsten quartz halogen
Humidity
10% to 85% relative humidity (non-condensing)
100 VA max, 130 VA max with injectors
Photodiode
Monochromator
200 to 999 nm, in 1 nm increments
°¤ 5 nm
Wavelength Precision
± 0.2 nm (standard deviation)
0.0001 OD (under Gen5 control)
Yes (under Gen5 control)
<1% at 2.0 OD
<3% at 2.5 OD
< 1% from 0 to 2.5 OD
0.03% at 230 nm
Reading Speed (Kinetic)
96 wells: 12 seconds
384 wells: 23 seconds
Fluorescence Specifications
Halogen
PMT
Bandpass filters
320 to 700 nm (low noise PMT)
320 to 850 nm (red-shifted PMT
> 6 decades
Fluorescein 2 pM
96 wells: 24 seconds
384 wells: 76 seconds
10 amol ATP
6, 12, 24, 48, 96, 384 wells
Read Methods
End point, kinetic, area scanning, absorbance spectral scanning (under Gen5 control)
Linear, orbital, double-orbital
96 wells: Abs 18 sec., Fl 17 sec., Lumi 120 sec.
384 wells: Abs 49 sec., Fl 48 sec., Lumi 420 sec.
Dimensions (H x W x D)
12 x 15 x 15 in. (30.5 x 38.1 x 38.1 cm)
27 lb. (12.3 kg.)
100-240V, 50/60Hz
Monochromator Bandwidth
2.4 nm
Tungsten halogen
Filter-based
Sensitivity (Top and Bottom)
Fluorescein 5 pM (1 fmol/well, 96-well plate)
30 amol ATP (flash)
Alpha Detection Specifications
300 amol of biotinylated LCK-P peptide
Read Speed
2 minutes (96-well plate)
End point, kinetic, spectral scanning, well-area scanning
Linear and orbital
4-Zone incubation to 50°C, ± 0.2°C at 37°C
Absorbance Photometric Performance
Monochromator, tunable in 1 nm increments
200 to 1000 nm
°¤ 4.0 nm
± 2.0 nm
Photometric Accuracy (Microplate)
< ±0.006 OD ±1%, 0 to 2 OD
Photometric Accuracy (Cuvette)
< ±0.005 OD ±1%, 0 to 2 OD
< ±0.003 OD ±1%, 0 to 2 OD
Baseline Flatness
1 nm increment selection
EX Wavelength Range
EM Wavelength Range
Wavelength Bandwidth (EX, EM)
Top-Read Optimized Sensitivity
5 pM (fluorescein) in 96 wells
Top-Read Guaranteed Sensitivity
15 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
360 to 850 nm
50 to 1450 µsec., 200 µsec. increments
Guaranteed Sensitivity
7 pM Eu-chelate
Luminescence (secondary mode)
50 fM alkaline phosphatase
General Photometric Performance
Plate Formats
Xenon flash lamp (1 joule/flash)
Photomultiplier tube (PMT)
96 wells: Abs 18 sec., Fl 15 sec.
384 wells: Abs 49 sec., Fl 45 sec.
Ambient +4°C to 45°C
< 1°C at 37°C set point
Temperature Accuracy
±1°C at 37°C set point
≤ 4.0 nm full width half maximum
± 2.0 nm across wavelength range
Fluorescence Intensity Performance
≤ 1 pM (fluorescein) in 96 wells≤ 1.5 pM (fluorescein) in 384 wells
< 5 pM (fluorescein) in 96 wells or cuvette< 20 pM in 384 wells
Luminescence Photometric Performance
< 2 fg/well for firefly luciferase in 96- and 384-well microplates (top read)
Crosstalk
< 0.5% in 96- and 384- well microplates
Optimized Sensitivity
≤ 43 pM ATP in 96 wells
≤ 75 pM ATP in 96 wells
Detectors
2 Photomultiplier tubes (PMT)
Ambient +2°C to 60°C
21.8 x 51.2 x 39 cm (8.6 x 22.5 x 15.4 in)
16.4 kg (36 lbs)
2 nm (230 to 285 nm), 4 nm (> 285 nm)
< 3% at 3.0 OD typical
< 0.5% at 2.0 OD typical
Tungsten halogen lamp
Double grating monochromators (top/bottom) and deep blocking filters/dichroic mirrors (top)
Monochromators: 250 to 850 nm
Filters: 200 to 700 nm (850 nm option)
Monochromators: 5 decades
Filters/Mirrors: > 6 decades
Top Monochromator: Fluorescein 2 pM typical (0.2 fmol/well 384-well plate)
Bottom Monochromator: Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate)
Filters/Mirrors: Fluorescein 1 pM typical (0.1 fmol/well 384-well plate)
Fluorescence Polarization Specifications
Deep blocking filters/dichroic mirrors (top)
400 to 700 nm (320 to 850 nm option)
3 mP at 1 nm fluorescein typical
Time-Resolved Fluorescence Specifications
High energy xenon flash lamp
200 to 700 nm (850 nm option)
Europium 60 fM typical with filters (6 amol/well in 384-well plate)
UV/Vis Photometric Performance:
200 ‚ 1000 nm
Monochromator, tunable in 1.0 nm increments
0 ‚ 4.0 OD
< ± 0.006 OD ± 1.0%, 0-2 OD
< ± 0.003 OD ± 1.0%, 0-2 OD
Fluorescence Photometric:
1 nm increments
EX 250 ‚ 850 nm
EM 360 ‚ 850 nm
Bandwidth (EX, EM)
9, 9 nm
3.0 fmol/well FITC 200µl in 96 wells
UV/Vis Photometric Performance
200 - 1000 nm
0 - 4.0 OD
1 nm increments EX 250 - 850 nmEM 360 - 850 nm
Time Resolved Fluorescence:
360 - 850 nm
0.5 fmol/well Eu-chelate (obtained with DELFIA reagent from PE by using a 384 well plate)
50 - 1450 µsec, 200µsec increments
Luminescnece:
10 amol/well alkaline phosphatase 200 µl/well (obtained with Emerald II reagent from ABI)
General Specifications:
96 well (Abs 18 sec, FI 15 sec) 384 well (Abs 49 sec, FI 45 sec)
Ambient +4 °C to 45°C
± 1 °C @ 37°C
± 0.5°C @ 37°C set point
Power
110 - 120 Vac 50/60 Hz
