Flow Channel Diameter

1.2 millimeters

Maximum Pressure

2 MPa, 20 bar, 290 psi

Valve type

Motorized rotary 8-way valve

Flow rate

Up to 100 milliliters per minute

Max Flow Rate

0.001 ‚ 100 ml/min

Max Pressure

12 ml chamber: 10 MPa at 0.1 ‚ 100 ml/min (100 bar, 1450 psi)
0.6, 2, and 5 ml chambers: 25 MPa at 0.001 ‚ 100 ml/min (250 bar, 3625 psi)
90 µl and 0.2 ml chambers: 35 MPa at 0.001 ‚ 5 ml/min (350 bar, 5075 psi)

pH Range

1 ‚ 13, 1 ‚ 14 (< 1 day exposure)

Viscosity

Max 5 cP

Internal Volume

6 interchangeable mixing chambers: 90 µl, 0.2, 0.6, 2, 5, and 12 ml

1 static chamber and 1 dynamic chamber

Operating Temperature Range

4°C to 40°C

Operating Humidity Range

< 95% non-condensing

Max Power Consumption

260 VA (210 W)

BTU

717

Operating Temperature Range

4°C to 40°C

Operating Humidity Range

< 95% non-condensing

Max Power Consumption

260 VA (210 W)

BTU

717

Temperature Range

4° to 40°C

Ambient Temperature

4° to 40°C

Dimensions

140 x 345 x 435 mm

Line Voltage

100-120 or 220-240 VAC

Line Frequency

50/60 Hz

Operating Pressure Max.

50 bar (5 MPa) (725 psi)

1 µS/cm-999.9 mS/cm

pH Stability Long Term

2-12

260 x 370 x 100 mm

Accuracy

The greater of ± 2% of full scale or ± 10 µS/cm in the range 1 µS/cm-300 mS/cm ± 0.1 pH unit, temperature compensated Max. 0.1 pH units within 0‚10 ml/min (pH measurement)

Baseline noise

± 0.5% of full scale calibrated range

Drift

Max. 0.02 pH/h (measured at pH 4.0) ±0.5 °C per 10 h 0.1 pH unit deviation/10 h

Cell Constant

50 ± 20 cm-1

Response Time

Max. 10 s (0‚95% of step) (pH measurements) Max. 3 s (0‚95% of step) (Conductivity measurement)

100-120/220-240 VAC

Maximum Flow Rate

0-5 ml/min per channel

5-10 ml/min at reduced degassing performance

Ambient Operating Temperature

0° to 55°C

Ambient Non-operating Temperature

-40° to 70°C

Power Consumption

30 W

Line Voltage

100-120 220-240 VAC

Line Frequency

50 r 60 Hz

Weight

7kg (15.4 lbs)

Dimensions (W x D x H)

13.5 x 17 x 3.1 in

3-Way Valves

3-port, PTFE, 114 µL internal volume, 6 µL dead volume from common port to the normally closed port, up to 200 mL/min with standard or large bore needle

Communication Interface

RS-232 or GSIOC; Inputs for start/advance and end/home; event mark output; one programmable output for control of peripheral devices

Data Acquisition

An input channel for analog-to-digital conversion; digital data is transmitted on the GSIOC

Detector Input

±100 mV full scale

Drop Counting

Up to 9,999 drops per fraction

maximum rate: 20 drops/sec

Event Marker

100 ms pulse

Front Panel

Two-line, 24-character display on backlit LCD. Five command, HELP, YES, NO and 0‚9 numeric hard keys; four soft keys

Maximum Collection Volume/Tube

32 mL (18 x 180 mm tubes) or 25 mL (18 x 150 mm)

Maximum Fractions

Up to 768 when configured with eight 96-well, 2-mL Whatman/Polyfiltronics microplates on a Code 17 rack)

Multiple-Column Collection

Simultaneous collection from up to 18 columns with installation of optional multiple column adaptor(s)

Multiple-Cycle Operation

Repetitive collection of each sample into same set of tubes or collection of each sample into different set of tubes
3-port, PTFE, 114 mL internal volume, 6 µL dead volume from the common port to the normally closed port, up to 200 mL/min with large bore needle

Number of Racks

Holds one Code 16 or Code 16D rack, one Code 17 rack, or up to four Code 20-Series racks (all racks must be the same code)

Operating Modes

Drop, Manual, Peak + Drop, Peak + Time, Time

Peak Detection

Adaptive-slope algorithm that applies user-specified parameters to accommodate drifting baselines, negative and asymmetrical peaks, or absolute threshold level that collects all peaks above specified mV value

Peak parameters: peak height or level, in mV and peak width at half-height, in minutes

Minimum peak height for fractionation: 0.1 mV full scale

Detector-collector delay: 0.01 minute increments

Power Requirements

120V, 50/60Hz, 0.5A

Programmable Time Units

From 0.01 to 99.99 minutes per tube with a 0.01 minute limit of resolution

Time-Based Programming

Up to 10 collection windows and 10 drain steps in any mode

Tube Change Time

100‚720 ms, center to center, depending on rack type

Dimensions (W x D x H)

47.9 x 46.4 x 33 cm (18.9 x 18.3 x 13 in.)

Max Flow Rate

10 ml/min per channel

Reduced performance above 5 ml/min

Number of Channels

4

Internal Volume

1 ml/channel

Materials in Contact with Solvent

PTE, PEEK

pH Range

1 ‚ 14

Analog Output (AUX)

For pressure monitoring, range 0 ‚ 3 V

Dimensions (W x D x H)

13.5 x 17 x 3.1 in (345 x 435 x 80 mm)

Electrical Requirements

100-120 or 220-240 VAC, 50/60 Hz

Temperature Range

Range 10° below ambient to 80° C

Temperature Stability

± 0.15°C

Temperature Accuracy

± 0.8°C

Column Capacity

Three 30cm columns

Warm-up/Cool Down

5 mins from ambient to 40°C

Temperature Range

4ƒš° to 40ƒš°C

Ambient Temperature

4ƒš° to 40ƒš°C

Temperature accuracy at ambient

temperatures < 25 ƒš°C and humidity < 50%

- 1ƒš°C to + 4 ƒš°C at a setpoint of 4 ƒš°C

Temperature accuracy at ambient

temperatures > 25 ƒš°C and/or humidity > 50%

- 1ƒš°C to + 5 ƒš°C at a setpoint of 4 ƒš°C

Dimensions

140 x 345 x 435 mm

Line Voltage

100-120 or 220-240 VAC

Line Frequency

50/60 Hz

Temperature Range

4°C to 40°C

Temperature Accuracy at Ambient Temperatures (< 25°C and Humidity < 50%)

3°C to 8°C at a setpoint of 4°C

Temperature Accuracy at Ambient Temperatures (< 25°C and Humidity < 60%)

3°C to 9°C at a setpoint of 4°C

Weight

46 lbs. (20.7 kg)

Hydraulic System

Two dual piston in series, with proprietary servo-controlled variable stroke drive, floating piston, active inlet valve, solvent selection valve and electronic flow control for flow rates up to 100 µl/min

Settable Column Flow Range

0.01 ‚ 20 µl/min

0.01 ‚ 100 µl/min (with the extended flow range kit)

0.001 ‚ 2.5 µl/min (with the electronic flow control bypassed)

Recommended Column Flow Range

1 ‚ 20 µl/min

10 ‚ 100 µl/min (with extended flow range kit)

0.1 ‚ 2.5 ml/min (with the electronic flow sensor bypassed)

Column Flow Precision

< 0.7 % RSD or 0.03 % SD (typically 0.4 % RSD or 0.02 % SD), at 10 µl/min and 50 µl/min column flow (based on RT, default setting)

Optimum Composition Range

1 to 99% or 5 µl/min per channel (primary flow), whatever is greater

Composition Precision

< 0.2 % SD, at 10 µl/min (20 µl flow sensor), 50 µl/min (100 µl flow sensor) and 1 ml/min (normal mode) default setting

Delay Volume

Typically 3 µl from the electronic flow control to the pump outlet for flow rates up to 20 µl/min.

Typically 12 µl from the electronic flow control to the pump outlet for flow rates up to 100 µl.

for flow rates up to 100 µl/min and electronic flow control active: primary flow path 180 - 480 µl without mixer, 600 - 900 µl with mixer (system pressure dependant)

Typically 180 to 480 µl (system pressure dependent) without mixer for flow rates up to 2.5 ml/min. (Mixer delay volume 420 µl)

Pressure Range

20 to 400 bar (5880 psi) system pressure

Compressibility Compensation

User-selectable, based on mobile phase compressibility

Recommended pH Range

1.0 ‚ 8.5, solvents with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries.

Control and Data Evaluation

Agilent ChemStation for LC

Analog Output

For pressure monitoring, 2 mV/bar, one output

Communications

Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional

Safety and Maintenance

Extensive diagnostics, error detection and display (through control module and Agilent ChemStation), leak detection, safe leak handling,

leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.

GLP Features

Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with user-settable limits and feedback messages. Electronic records of maintenance and errors.

Housing

All materials recyclable.

Temperature Range

Range 10° below ambient to 80° C

Temperature Stability

± 0.15°C

Temperature Accuracy

± 0.8°C

Column Capacity

Three 30cm columns

Warm-up/Cool Down

5 mins from ambient to 40°C

95 fractions in 10-18 mm tubes

< 100 ml/min

pH Stability Long Term

1 ‚ 13

pH Stability Short Term

1 ‚ 14

Program Memory

99 programs

95 x 10-18 mm (Standard)

175 x 12 mm and 40 x 30 mm (Optional)

Time Mode

0.01 ‚ 9.999 min

Volume Mode

0.1 ‚ 999 ml

Voltage

100 ‚ 240 V

Flow Rate

Up to 50 ml

Maximum Pressure

20 bar (2 MPa, 290 psi)

Operating Temperature

4° to 40°C

Dimensions (H x W x D)

158 x 148 x 99 mm

Weight

14.2 kg (31.3 lbs)

Dimensions (H x W x D)

200 x 345 x 435 mm (8 x 13.5 x 17 in)

Line Voltage

Line frequency

100 ‚ 120 or 220 ‚ 240 VAC, ± 10 %

50 or 60 Hz, ± 5 %

Power consumption (apparent power)

Power consumption (active power)

300 VA max.

200 W max.

Ambient operating temperature

Ambient non-operating temperature

4 ‚ 55 °C (41 ‚ 131 °F)

40 to 70 °C (-4 to 158 °F)

Humidity

< 95 %, at 25 ‚ 40 °C (77 ‚ 104 °F) non-condensing

Tray

Thermostattable Tray for 100 x 2 ml vials Microvials (100 or 300 µl) with sleeves (reduced cooling performance with microvials)

Settable injection volume

0.01 to 8 µl with small loop capillary

0.01 to 40 µl with extended loop capillary

Precision

Typically < 0.5 % RSD from 5 to 40 µl Typically < 1 % RSD from 1 to 5 µl Typically < 3 % RSD from 0.2 to 1 µl

Minimum sample volume

1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvials

Carryover

Typically < 0.1 % without automated needle wash Typically < 0.05 % with external needle cleaning and 1 µl injection volume

Sample viscosity range

0.2 - 5 cp

Recommended pH-range

1.0 - 8.5 solvent with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries

Material in contact with solvent

Stainless steel, sapphire, PTFE, PEEK, fused silica, Vespel

GLP features

Early maintenance feedback (EMF), electronic records of maintenance and errors

Communications

Controller-area network (CAN), GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output

Housing

All materials are recyclable

95 fractions in 10-18 mm tubes

< 100 ml/min

pH Stability Long Term

1 ‚ 13

pH Stability Short Term

1 ‚ 14

Program Memory

99 programs

95 x 10-18 mm (Standard)

175 x 12 mm and 40 x 30 mm (Optional)

Time Mode

0.01 ‚ 9.999 min

Volume Mode

0.1 ‚ 999 ml

Voltage

100 ‚ 240 V

GLP Features

Early maintenance feedback (EMF), electronic records of maintenance and errors

Communications

Controller-area network (CAN). GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output

Safety Features

Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display

Injection Range

0.1 ‚ 100 µl in 0.1 µl increments Up to 1500 µl with multiple draw (hardware modification required)

Replicate Injections

1 ‚ 99 from one vial

Precision

Typically < 0.5 % RSD of peak areas from 5 ‚ 100 µl

Typically < 1 % RSD of peak areas from 1 ‚ 5 µl

Minimum Sample Volume

1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial

Carryover

Typically < 0.1 %, < 0.05 % with external needle cleaning

Sample Viscosity Range

0.2 ‚ 50 cp

Replicate Injections Per Vial

1 ‚ 99

Sample Capacity

100 ƒ” 2-ml vials in 1 tray

40 ƒ” 2-ml vials in ½ tray

15 ƒ” 6-ml vials in ½ tray (Agilent vials only)

Injection Cycle Time

Typically 50 s depending on draw speed and injection volume

Setable Flow Range

Setpoints 0.001 to 5 ml/min, in 0.001 ml/min increments

Flow Range

0.1 to 5 ml/min

Flow Precision

°¤ 0.07% RSD or °¤ 0.02 min SD

Flow Accuracy

± 1% or 10 ul/min

Pressure

Operating range 0 to 400 bar (0 to 5880 psi) up to 5 ml/min

Pressure Pulsation

< 2% amplitude (typically < 1%) at 1 ml/min isopropanol, at all pressures > 1 MPa

Compressibility Compensation

User-selectable, based on mobile phase compressibility

Hydraulic System

Two dual pistons in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve

Recommended pH Range

1.0 to 12.5; solvents with pH < 2.3 should not contain acides with attack stainless steel

Gradient Formation

High-pressure binary mixing, delay volume 180 to 480 ul without mixer, 600 to 900 ul with mixer, dependent upon back pressure

Composition Range

1 to 99% or 5 ul/min per channel

Composition Precision

°¤ 0.5% absolute

Composition Accuracy

°¤ 0.15% RSD at 1 ml/min

Flow Rate

Up to 50 ml

Maximum Pressure

20 bar (2 MPa, 290 psi)

Operating Temperature

4° to 40°C

Dimensions (H x W x D)

158 x 148 x 99 mm