Max Flow Rate

100 ml/min

Max Pressure

25 MPa (250 bar, 3600 psi)

Back Pressure

< 40 kPa at 100 ml/min with water

Leakage

<0.1 ul/min at 25 MPa

pH Stability Range

1-13, 1-14 (<1 day exposure)

Viscosity

Max 5 cP

Switch Time

< 260 ms between two adjacent positions

Operating Life Time

> 50,000 cycles, two adjacent positions

Flow Channel Diameter

1.2 mm

Max Flow Rate

100 ml/min

Max Pressure

25 MPa (250 bar, 3600 psi)

Back Pressure

<50 kPa at 100 ml/min with water

Leakage

<0.1 ul/min at 25 MPa

pH Stability Range

1-13, 1-14 (<1 day exposure)

Viscosity

Max 5 cP

Switch Time

<260 ms between two adjacent positions

3-Way Valves

3-port, PTFE, 114 µL internal volume, 6 µL dead volume from common port to the normally closed port, up to 200 mL/min with standard or large bore needle

Communication Interface

RS-232 or GSIOC; Inputs for start/advance and end/home; event mark output; one programmable output for control of peripheral devices

Data Acquisition

An input channel for analog-to-digital conversion; digital data is transmitted on the GSIOC

Detector Input

±100 mV full scale

Drop Counting

Up to 9,999 drops per fraction

maximum rate: 20 drops/sec

Event Marker

100 ms pulse

Front Panel

Two-line, 24-character display on backlit LCD. Five command, HELP, YES, NO and 0–9 numeric hard keys; four soft keys

Maximum Collection Volume/Tube

32 mL (18 x 180 mm tubes) or 25 mL (18 x 150 mm)

Maximum Fractions

Up to 768 when configured with eight 96-well, 2-mL Whatman/Polyfiltronics microplates on a Code 17 rack)

Multiple-Column Collection

Simultaneous collection from up to 18 columns with installation of optional multiple column adaptor(s)

Multiple-Cycle Operation

Repetitive collection of each sample into same set of tubes or collection of each sample into different set of tubes
3-port, PTFE, 114 mL internal volume, 6 µL dead volume from the common port to the normally closed port, up to 200 mL/min with large bore needle

Number of Racks

Holds one Code 16 or Code 16D rack, one Code 17 rack, or up to four Code 20-Series racks (all racks must be the same code)

Operating Modes

Drop, Manual, Peak + Drop, Peak + Time, Time

Peak Detection

Adaptive-slope algorithm that applies user-specified parameters to accommodate drifting baselines, negative and asymmetrical peaks, or absolute threshold level that collects all peaks above specified mV value

Peak parameters: peak height or level, in mV and peak width at half-height, in minutes

Minimum peak height for fractionation: 0.1 mV full scale

Detector-collector delay: 0.01 minute increments

Power Requirements

120V, 50/60Hz, 0.5A

Programmable Time Units

From 0.01 to 99.99 minutes per tube with a 0.01 minute limit of resolution

Time-Based Programming

Up to 10 collection windows and 10 drain steps in any mode

Tube Change Time

100-720 ms, center to center, depending on rack type

Dimensions (W x D x H)

47.9 x 46.4 x 33 cm (18.9 x 18.3 x 13 in.)

10 ml/min per channel

Reduced performance above 5 ml/min

10 ml/min per channel

Reduced performance above 5 ml/min

0.01 to 20 µl/min

0.01 to 100 µl/min (with the extended flow range kit)

0.001 to 2.5 µl/min (with the electronic flow control bypassed)

1 to 20 µl/min

10 to 100 µl/min (with extended flow range kit)

0.1 to 2.5 ml/min (with the electronic flow sensor bypassed)

Number of Channels

4

Internal Volume per Channel

Typically 1 ml per channel

Materials in Contact with Solvent

PTFE, FEP, PEEK

pH Range

1 to 14

RS-232 Output

For diagnostic purposes

Weight

14.2 kg (31.3 lbs)

Dimensions (H x W x D)

200 x 345 x 435 mm (8 x 13.5 x 17 in)

Line Voltage

Line frequency

100 to 120 or 220 to 240 VAC, ± 10 %

50 or 60 Hz, ± 5 %

Power consumption (apparent power)

Power consumption (active power)

300 VA max.

200 W max.

Ambient operating temperature

Ambient non-operating temperature

4 to 55C (41 to 131F)

40 to 70C (-4 to 158F)

Humidity

< 95 %, at 25 to 40C (77 to 104F) non-condensing

Tray

Thermostattable Tray for 100 x 2 ml vials Microvials (100 or 300 µl) with sleeves (reduced cooling performance with microvials)

Settable injection volume

0.01 to 8 µl with small loop capillary

0.01 to 40 µl with extended loop capillary

Precision

Typically < 0.5 % RSD from 5 to 40 µl Typically < 1 % RSD from 1 to 5 µl Typically < 3 % RSD from 0.2 to 1 µl

Minimum sample volume

1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvials

Carryover

Typically < 0.1 % without automated needle wash Typically < 0.05 % with external needle cleaning and 1 µl injection volume

Sample viscosity range

0.2 - 5 cp

Recommended pH-range

1.0 - 8.5 solvent with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries

Material in contact with solvent

Stainless steel, sapphire, PTFE, PEEK, fused silica, Vespel

GLP features

Early maintenance feedback (EMF), electronic records of maintenance and errors

Communications

Controller-area network (CAN), GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output

Housing

All materials are recyclable

40 x 30 mm (included)

95 x 18 mm

175 x 12 mm