Trigger Modes
Time slices, Peak (threshold, up- /downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with G1323BControl Module)
Operating Modes
Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste
Test Tube/Plate Sizes
Minimum 48 mm to 75 mm maximum
Maximum Tube Volume
ca. 45 ml
Maximum Flow Rate
100 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve)
Delay Volumes [μl]
Fraction collector inlet to diverter valve: -500 (typical, depends on length of the tubing)
Diverter valve: ~15
Diverter valve to needle: ~110
Needle: -~5
Delay Calibration Sensor
Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode
Diverter Valve
3/2 Diverter valve with low internal volume (15 μl), switching time < 100 ms, maximum operating pressure 6 bar
Maximum Capacity
3 fraction collectors in parallel plus one recovery fraction collector connected via 12-Position, 13-Port Selector valve
Interfaces
Controller-area network (CAN). optional; LAN or external contacts interface RS232C, APG-remote (for remote start / stop signals to / from other modules) Interface to G1330A Thermostat CAN-DC-out for operation of Agilent approved external devices like valves
Safety Features
Leak detection and safe leak handling, error detection and display, exhaust fan for fume extraction of hazardous vapors
3-Way Valves
3-port, PTFE, 114 µL internal volume, 6 µL dead volume from common port to the normally closed port, up to 200 mL/min with standard or large bore needle
Communication Interface
RS-232 or GSIOC; Inputs for start/advance and end/home; event mark output; one programmable output for control of peripheral devices
Data Acquisition
An input channel for analog-to-digital conversion; digital data is transmitted on the GSIOC
Detector Input
±100 mV full scale
Drop Counting
Up to 9,999 drops per fraction
maximum rate: 20 drops/sec
Event Marker
100 ms pulse
Front Panel
Two-line, 24-character display on backlit LCD. Five command, HELP, YES, NO and 0–9 numeric hard keys; four soft keys
Maximum Collection Volume/Tube
32 mL (18 x 180 mm tubes) or 25 mL (18 x 150 mm)
Maximum Fractions
Up to 768 when configured with eight 96-well, 2-mL Whatman/Polyfiltronics microplates on a Code 17 rack)
Multiple-Column Collection
Simultaneous collection from up to 18 columns with installation of optional multiple column adaptor(s)
Multiple-Cycle Operation
Repetitive collection of each sample into same set of tubes or collection of each sample into different set of tubes3-port, PTFE, 114 mL internal volume, 6 µL dead volume from the common port to the normally closed port, up to 200 mL/min with large bore needle
Number of Racks
Holds one Code 16 or Code 16D rack, one Code 17 rack, or up to four Code 20-Series racks (all racks must be the same code)
Drop, Manual, Peak + Drop, Peak + Time, Time
Peak Detection
Adaptive-slope algorithm that applies user-specified parameters to accommodate drifting baselines, negative and asymmetrical peaks, or absolute threshold level that collects all peaks above specified mV value
Peak parameters: peak height or level, in mV and peak width at half-height, in minutes
Minimum peak height for fractionation: 0.1 mV full scale
Detector-collector delay: 0.01 minute increments
Power Requirements
120V, 50/60Hz, 0.5A
Programmable Time Units
From 0.01 to 99.99 minutes per tube with a 0.01 minute limit of resolution
Time-Based Programming
Up to 10 collection windows and 10 drain steps in any mode
Tube Change Time
100-720 ms, center to center, depending on rack type
Dimensions (W x D x H)
47.9 x 46.4 x 33 cm (18.9 x 18.3 x 13 in.)
