Setable Flow Range
0.001 ƒš‚Å“ 10 ml/min, in 0.001 ml/min increments
Flow Range
0.2 ƒš‚Å“ 10.0 ml/min
Flow Precision
ƒš£ 0.07% RSD, or ƒš£ 0.02 min SD whatever is greater, based on retention time at constant room temperature
Flow Accuracy
ƒš± 1% or 10 ƒšµl/min whatever is greater
Pressure
Operating range 0 ƒš‚Å“ 40 MPa (0 ƒš‚Å“ 400 bar, 0 ƒš‚Å“ 5880 psi) up to 5 ml/min
Operating range 0 ƒš‚Å“ 20 MPa (0 ƒš‚Å“ 200 bar, 0 ƒš‚Å“ 2950 psi) up to 10 ml/min
Pressure Pulsation
< 2% amplitude (typically < 1%), at 1 ml/min isopropanol, at all pressures > 1 MPa (10 bar)
Recommended pH Range
1.0 ƒš‚Å“ 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel
Gradient Formation
Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve
Delay volume 800 ƒš‚Å“ 1100 ƒšµl, dependent on back pressure
Composition Range
0 ƒš‚Å“ 95% or 5 ƒš‚Å“ 100%, user selectable
Composition Precision
< 0.2% SD, at 0.2 and 1 ml/min
Dimensions (H x W x D)
5.5 x 13.5 x 17 in
Electrical Requirements
100-120 or 22-240 VAC, 50/60 Hz, 220 VA
Hydraulic system
Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
Setable flow range
Setpoints 0.001 ‚ 5 ml/min, in 0.001 ml/min increments
Flow range
0.1 ‚ 5.0 ml/min
Flow precision
£ 0.07% RSD, or £ 0.02 min SD whatever is greater, based on retention time at constant room temperature
Flow accuracy
± 1% or 10 µl/min whatever is greater
Operating range 0 400 bar (0 ‚ 5880 psi) up to 5 ml/min
Pressure pulsation
< 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
Compressibility compensation
User-selectable, based on mobile phase compressibility
Recommended pH range
1.0 ‚ 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient formation
High-pressure binary mixing, delay volume 180 ‚ 480 µl without mixer, 600 ‚ 900 µl with mixer, dependent on back pressure
Composition range
1 ‚ 99 % or 5 µl/min per channel, whatever is greater
Composition precision
£ 0.5% absolute
Composition accuracy
± 0.15% RSD, at 1 ml/min
0.001 ƒš‚Å“ 10 mL/min, in 0.001 mL/min increments
0.2 ƒš‚Å“ 10.0 mL/min
ƒ‚°¤ 0.07% RSD, or ƒ‚°¤ 0.02 min SD whatever is greater, based on retention time at constant room temperature
Pressure Operating Range
0 ƒš‚Å“ 60 MPa (0 ƒš‚Å“ 600 bar, 0 ƒš‚Å“ 8,700 psi) up to 5 mL/min
0 ƒš‚Å“ 20 MPa (0 ƒš‚Å“ 200 bar, 0 ƒš‚Å“ 2,950 psi) up to 10 mL/min
< 2% amplitude or < 3 bar at 1 mL/min isopropanol, at all pressure > 1 MPa (10 bar, 145 psi)
1.0 ƒš‚Å“ 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
< 0.2% RSD or < 0.04 min SD
100 - 240V, 50/60 Hz, 180 VA
0.05 - 5.0 mL/min
Setpoints 0.001 to 5 ml/min, in 0.001 ml/min increments
0.1 to 5 ml/min
°¤ 0.07% RSD or °¤ 0.02 min SD
± 1% or 10 ul/min
Operating range 0 to 400 bar (0 to 5880 psi) up to 5 ml/min
< 2% amplitude (typically < 1%) at 1 ml/min isopropanol, at all pressures > 1 MPa
Compressibility Compensation
Hydraulic System
Two dual pistons in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
1.0 to 12.5; solvents with pH < 2.3 should not contain acides with attack stainless steel
High-pressure binary mixing, delay volume 180 to 480 ul without mixer, 600 to 900 ul with mixer, dependent upon back pressure
1 to 99% or 5 ul/min per channel
°¤ 0.5% absolute
Composition Accuracy
°¤ 0.15% RSD at 1 ml/min
0.001 ‚ 10 ml/min, in 0.001 ml/min increments
0.2 ‚ 10.0 ml/min
< 0.3% RSD (usually 0.15%), based on retention time, at 1 ml/min
Operating range 0 ‚ 40 MPa (0 ‚ 400 bar, 0 ‚ 5880 psi) up to 5 ml/min
Operating range 0 ‚ 20 MPa (0 ‚ 200 bar, 0 ‚ 2950 psi) up to 10 ml/min
1.0 ‚ 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel
Delay volume 800 ‚ 1100 µl, dependent on back pressure
0 ‚ 95% or 5 ‚ 100%, user selectable
Two dual piston in series, with proprietary servo-controlled variable stroke drive, floating piston, active inlet valve, solvent selection valve and electronic flow control for flow rates up to 100 µl/min
Settable Column Flow Range
0.01 ‚ 20 µl/min
0.01 ‚ 100 µl/min (with the extended flow range kit)
0.001 ‚ 2.5 µl/min (with the electronic flow control bypassed)
Recommended Column Flow Range
1 ‚ 20 µl/min
10 ‚ 100 µl/min (with extended flow range kit)
0.1 ‚ 2.5 ml/min (with the electronic flow sensor bypassed)
Column Flow Precision
< 0.7 % RSD or 0.03 % SD (typically 0.4 % RSD or 0.02 % SD), at 10 µl/min and 50 µl/min column flow (based on RT, default setting)
Optimum Composition Range
1 to 99% or 5 µl/min per channel (primary flow), whatever is greater
< 0.2 % SD, at 10 µl/min (20 µl flow sensor), 50 µl/min (100 µl flow sensor) and 1 ml/min (normal mode) default setting
Delay Volume
Typically 3 µl from the electronic flow control to the pump outlet for flow rates up to 20 µl/min.
Typically 12 µl from the electronic flow control to the pump outlet for flow rates up to 100 µl.
for flow rates up to 100 µl/min and electronic flow control active: primary flow path 180 - 480 µl without mixer, 600 - 900 µl with mixer (system pressure dependant)
Typically 180 to 480 µl (system pressure dependent) without mixer for flow rates up to 2.5 ml/min. (Mixer delay volume 420 µl)
Pressure Range
20 to 400 bar (5880 psi) system pressure
1.0 ‚ 8.5, solvents with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries.
Control and Data Evaluation
Agilent ChemStation for LC
Analog Output
For pressure monitoring, 2 mV/bar, one output
Communications
Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
Safety and Maintenance
Extensive diagnostics, error detection and display (through control module and Agilent ChemStation), leak detection, safe leak handling,
leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
GLP Features
Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with user-settable limits and feedback messages. Electronic records of maintenance and errors.
Housing
All materials recyclable.
