Type
Specification
Detection type
1024-element photodiode array
Light Source
Deuterium and tungsten lamps
Wavelength range
190 ‚ 950 nm
Short term noise (ASTM) single and multi-Wavelength
± 1 ƒ” 10-5 AU at 254 and 750 nm
Drift
2 ƒ” 10-3 AU/hr at 254 nm
Linear absorbance
range
> 2 AU (upper limit)
Wavelength Accuracy
± 1 nm
Wavelength bunching
1 ‚ 400 nm
Slit width
1, 2, 4, 8, 16 nm
Diode Width
< 1 nm
Flow Cells
Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum High pressure: 1.7 µl volume, 6 mm cell path length and 400 bar (5880 psi) pressure maximum 500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
Control and data evaluation
Agilent ChemStation for LC
Analog outputs
Recorder/integrator: 100 mV or 1 V, output range 0.001 ‚ 2 AU, two outputs
Communications
Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
Safety and maintainence
Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas
GLP features
Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter.
Housing
All materials recyclable
± 1 x 10-5 AU at 254 and 750 nm
2 x 10-3 AU/hr at 254 nm
Refractive Index Range
1.00 to 1.75 RIU, calibrated
Measurement Range
± 600 x 10-6 RIU
Optical Zeroing
via set screw
Optics Temperature Control
5°C above ambient to 55°C
Sample Cell
Volume 8 µL, maximum pressure 5 bar (0.5 Mpa), maximum flow rate 5 mL/min
Valves
Automatic purge and automatic solvent recycle
Volumes
Inlet port to sample cell: 62 µL
Inlet port to outlet port: 590 µL
Liquid Contact Materials
316 stainless steel, teflon and quartz glass
pH Range
Short Term Noise
< ± 2.5 x 10-9 RIU
Time Programmable Parameters
Detection Type
Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis
Performance Specifications
Single wavelength operation: ‚ RAMAN (H2O) > 500 (noise reference measured at signal) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell ‚ RAMAN (H2O) > 3000 (noise reference measured at dark value) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell Dual wavelength operation: RAMAN (H2O) > 300 Ex 350 nm, Em 397 nm and Ex 350 nm, Em 450 nm, standard flow cell.
Light source
Xenon Flash Lamp, normal mode 20 W, economy mode 5 W, lifetime 4000 h
Pulse frequency
296 Hz for single signal mode 74 Hz for economy mode
Excitation Monochromator
Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 300 nm
Emission Monochromator
Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 400 nm
Reference System:
in-line excitation measurement
Timetable programing
up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters
Spectrum acquisition:
Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200 ‚ 400 nm, 10 nm step) Step size: 1 ‚ 20 nm Spectra storage: All
Wavelength characteristic
Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting
Flow cells
Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz
Wavelength Range
Short Term Noise (ASTM) Single and Multi-Wavelength
± 1 ƒ” 10^-5AU at 254 and 750 nm
2 ƒ” 10^-3AU/hr at 254 nm
Linear Absorbance Range
Wavelength Bunching
Slit Width
Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum
Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum
High pressure: 1.7 µl volume, 6 mm cell path length and 400 bar (5880 psi) pressure maximum
500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
Control and Data Evaluation
Analog Outputs
Safety and Maintenance
Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
GLP Features
Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter
All materials recyclable.
