Setable Flow Range

0.001 to 10 ml/min, in 0.001 ml/min increments

Flow Range

0.2 to 10.0 ml/min

Flow Precision

< 0.3% RSD (usually 0.15%), based on retention time, at 1 ml/min

Pressure

Operating range 0 to 40 MPa (0 to 400 bar, 0 to 5880 psi) up to 5 ml/min

Operating range 0 to 20 MPa (0 to 200 bar, 0 to 2950 psi) up to 10 ml/min

Pressure Pulsation

< 2% amplitude (typically < 1%), at 1 ml/min isopropanol, at all pressures > 1 MPa (10 bar)

Recommended pH Range

1.0 to 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel

Gradient Formation

Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve

Delay volume 800 to 1100 µl, dependent on back pressure

Composition Range

0 to 95% or 5 to 100%, user selectable

Composition Precision

< 0.2% SD, at 0.2 and 1 ml/min

Dimensions (H x W x D)

5.5 x 13.5 x 17 in

Electrical Requirements

100 to 120 or 22 to 240 VAC, 50/60 Hz, 220 VA

10 ml/min per channel

Reduced performance above 5 ml/min

5 minutes from ambient to 40°C

10 minutes from 40°C to 20°C

0 to 5 ml/min per channel

5 to 10 ml/min at reduced degassing performance

0.01 to 20 µl/min

0.01 to 100 µl/min (with the extended flow range kit)

0.001 to 2.5 µl/min (with the electronic flow control bypassed)

1 to 20 µl/min

10 to 100 µl/min (with extended flow range kit)

0.1 to 2.5 ml/min (with the electronic flow sensor bypassed)

Weight

14.2 kg (31.3 lbs)

Dimensions (H x W x D)

200 x 345 x 435 mm (8 x 13.5 x 17 in)

Line Voltage

Line frequency

100 to 120 or 220 to 240 VAC, ± 10 %

50 or 60 Hz, ± 5 %

Power consumption (apparent power)

Power consumption (active power)

300 VA max.

200 W max.

Ambient operating temperature

Ambient non-operating temperature

4 to 55C (41 to 131F)

40 to 70C (-4 to 158F)

Humidity

< 95 %, at 25 to 40C (77 to 104F) non-condensing

Tray

Thermostattable Tray for 100 x 2 ml vials Microvials (100 or 300 µl) with sleeves (reduced cooling performance with microvials)

Settable injection volume

0.01 to 8 µl with small loop capillary

0.01 to 40 µl with extended loop capillary

Precision

Typically < 0.5 % RSD from 5 to 40 µl Typically < 1 % RSD from 1 to 5 µl Typically < 3 % RSD from 0.2 to 1 µl

Minimum sample volume

1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvials

Carryover

Typically < 0.1 % without automated needle wash Typically < 0.05 % with external needle cleaning and 1 µl injection volume

Sample viscosity range

0.2 - 5 cp

Recommended pH-range

1.0 - 8.5 solvent with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries

Material in contact with solvent

Stainless steel, sapphire, PTFE, PEEK, fused silica, Vespel

GLP features

Early maintenance feedback (EMF), electronic records of maintenance and errors

Communications

Controller-area network (CAN), GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output

Housing

All materials are recyclable

10 fg Anthracene, Ex=250 nm, Em=400 nm

RAMAN (H2O) > 200 (FLF rev A)

RAMAN (H2O) > 400 (FLF rev >A)

Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 second responsetime)

Range: settable 200 - 700 nm and zero-order

Bandwidth: 20 nm (fixed)

Monochromator: concave holographic grating, F/1.6, blaze: 300 nm

Range: settable 280 - 900 nm and zero-order

Bandwidth: 20 nm (fixed)

Monochromator: concave holographic grating, F/1.6, blaze: 400 nm

Repeatability: ± 0.2 nm

Accuracy: ± 3 nm setting