|
|
|
|
Waters 2695 HPLC System w/ PDA Detector
The Waters 2695 HPLC System integrates solvent and sample
management with consistent system-to-system performance and high reproducibility.
The Waters chromatography system ensures the reliability of results through high
injection precision for precise quantification and exceptional retention time
repeatability for accurate peak identification. The 2695s solvent delivery
system features quaternary blending with a fixed delay volume for consistent
and predictable results, regardless of system back pressure. With pulse-free
solvent delivery through the serial flow path without the use of dampeners and accurate
flow from online solvent degassing and automated solvent compressibility for, the
Waters HPLC system truly features advanced solvent delivery capabilities.
The 2695 HPLC System includes the Waters 2996 HPLC PhotoDiode Array Detector. The 2996 PDA Detector is the most versatile HPLC photodiode array detector available with the highest sensitivity; it operates exclusively with Waters Empower software to provide complete spectral acquisition and results management.
Waters HPLC systems are built for various applications and
routinely used in analytical environments, such as food safety, forensic, research
and development, quality control, and chemistry and pharmaceutical laboratories.
All of our equipment is fully tested to perform at factory
specifications before being shipped.
The Waters 2695 HPLC System w/ PDA Detector includes:
- > 2996 PDA Detector
- > PCI Card
- > Column Heater
- > Windows 7 Datastation with Empower 3 Software
- > Flat Screen Monitor
- > 4 Peak Calibration/Performance Reports
- > Connection Cables
- > Power Cords
- > Operator's Manual
- > Full 180 Day Parts and Labor Warranty
Waters 2695 HPLC System Specifications
Number of solvents
|
One to four
|
Solvent conditioning
|
Vacuum degas, two (2) operating modes, four (4) chambers, ?<500 uL internal volume per chamber>
<500uL interval volume per chamber
|
Programmable flow rate range
|
0.000 and 0.010 to 10.000 mL/min in 0.001-mL/min. increments
|
Typical operating flow rate range
|
0.050 to 5.000 mL/min in 0.001-mL/min increments
|
Compressibility compensation
|
Automatic and continuous
|
Effective system delay volume
|
<650 L, independent of backpressure @ 1 mL/min
|
Plunger seal wash
|
Integra, active, programmable
|
Gradient profiles
|
Eleven (11) gradient curves [including linear, step (2 curves),
concave (4 curves), and convex (4 curves)]
|
Dry prime/wet prime
|
Automatic, front panel control
|
Flow ramping
|
Time (0.01 to 30.00 min in 0.01-min increments) to reach maximum flow
rate
|
Maximum operating pressure
|
5000 psi (345 bar) (0.010 to 3.000 mL/min) Programmable upper and
lower limits. Pressure fall-off at >3.000 mL/min.
|
Pressure ripple
|
?2.5% (1 mL/min, degassed methanol, at 1700 psi backpressure)
|
Composition accuracy
|
0.5% absolute, independent of backpressure (Proportioning Valve Pair
Test [degassed methanol:methanol/propylparaben, 2 mL/min, 254 nm])
|
Composition precision
|
?0.15% RSD or ?0.02 min SD, whichever is greater, based on retention
time. (Degassed methanol:water 60:40 Dial-a-Mix, 1 mL/min, six replicates,
phenone mix, 254 nm.)
|
Flow precision
|
?0.075% RSD or ?0.02 min SD, based on retention time (N = 6) or
volumetric measures (0.200 to 5.000 mL/min), isocratic premix
|
Flow accuracy
|
1% or 10 L/min, whichever is greater, (0.200 to 5.000 mL/ min),
degassed methanol, at 600 psi backpressure
|
Waters 2695 HPLC System Manual
Click Here to View all the Technical Specs for the Waters 2695
- Verify uniformity of main and residential firmware across all components, updating as necessary to ensure compatibility
- Confirm successful communication between software and the HPLC system, validating data transfer integrity
- Check the degasser’s internal vacuum to confirm it reaches the specified vacuum level for optimal performance
- Replace inlet, outlet, purge valve, and pump seals in the system pump to maintain reliable operation
- Ensure system pump passes pressure and leak tests, confirming secure and efficient performance
- Replace all capillary tubing with new OEM stainless steel tubing for durability and precision
- Remove autosampler transport assembly, replace X and Y bearings, and thoroughly clean carrier rails to remove debris and contaminants
- Replace metering valve seal, autosampler needle, and needle seat for consistent sampling accuracy
- Test column compartment switching valve functionality and replace its seal if necessary
- Confirm column compartment passes thermostat test to maintain precise temperature control
- Remove and clean internal detector optics for clear and accurate signal detection
- Replace UV and Vis detector lamps to ensure optimal light source stability and performance
- Verify flow cell absorbance meets manufacturer’s specifications for accurate measurement
- Confirm system response to isocratic test sample, ensuring stable and reproducible results
Technician Inspection and Recertification Checklist: HPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
Task
|
Completed?
|
Remarks?
|
Verify firmware uniformity and update as necessary
|
Completed
|
|
Confirm software communication with HPLC system
|
Completed
|
|
Check degasser vacuum level for optimal performance
|
Completed
|
|
Replace inlet, outlet, purge valve, and pump seals
|
Completed
|
|
Ensure pump passes pressure and leak tests
|
Completed
|
|
Replace all capillary tubing with OEM stainless steel tubing
|
Completed
|
|
Remove autosampler transport assembly, replace bearings, and clean rails
|
Completed
|
|
Replace metering valve seal, autosampler needle, and needle seat
|
Completed
|
|
Test column compartment switching valve and replace seal if needed
|
Completed
|
|
Confirm thermostat test in column compartment
|
Completed
|
|
Clean internal detector optics
|
Completed
|
|
Replace UV and Vis detector lamps
|
Completed
|
|
Verify flow cell absorbance meets manufacturer’s specifications
|
Completed
|
|
Confirm system response to isocratic test sample
|
Completed
|
|
To View More of Our Recertification Protocol Click Here
|
|
|
|
|
|
|