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Thermo Scientific NanoDrop 2000 Spectrophotometer
Wavelength Range
190 to 840 nm
Minimum Sample Size
0.5 ul
- Measures up to 2 µl samples with high accuracy and reproducibility
- NanoDrop patented sample retention system enables analysis of highly concentrated samples without dilution
- Handles sample concentrations up to 200 times higher than standard cuvette systems
- Ideal for nucleic acid, DNA, RNA, and protein quantification
- Absorbance accuracy of 2% at 0.76 absorbance at 257 nm
- Auto-ranging sample pathlength from 1 mm to 0.05 mm for optimal performance
- Utilizes a xenon flash lamp and a 2048-element linear silicon CCD array for fast, precise measurements
- Delivers a detection limit of 2 ng/L and measures maximum concentrations up to 15,000 ng/L for dsDNA
The Thermo Scientific NanoDrop 2000 is a full-spectrum spectrophotometer that measures up to 2 ul samples with high accuracy and reproducibility.
This system offers the convenience of the NanoDrop patented sample retention system. This sample retention system employs surface tension to hold the sample in place between two optical fibers. This enables the measurement of very highly concentrated samples without the need for dilutions. Using this technology combined with a wavelength range of 190 to 840 nm, the NanoDrop 2000 has the ability to measure sample concentration up to 200 times more concentrated than samples measured using a standard cuvette system. (The NanoDrop 2000 model does not accept cuvettes, only the NanoDrop 2000c model is capable of this).
Applications include measuring: Nucleic Acid Quantification, DNA Quantification, RNA Quantification, and Protein Quantification.
All of our equipment is fully tested to perform at factory
specifications before being shipped.
The Thermo Scientific NanoDrop 2000 Spectrophotometer includes:
- > Windows 10 Pro Data Station with Nanodrop Software Preloaded
- > CF-1 Calibration Report
- > Power Cord
- > Full 180 Day Parts and Labor Warranty
Thermo Scientific NanoDrop 2000 Spectrophotometer Specifications
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Wavelength Range
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190 to 840 nm
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Wavelength Accuracy
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1 nm
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Absorbance Accuracy
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2% (at 0.76 absorbance at 257 nm)
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Absorbance Range
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0.02 to 300 absorbance (10 mm equivalent)
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Absorbance Precision
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0.002 absorbance (1 mm path)
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Minimum Sample Size
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0.5 ul
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Pathlength
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1 mm (auto-ranging to 0.05 mm)
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Light Source
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Xenon flash lamp
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Detector Type
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2048-element linear silicon CCD array
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Spectral Resolution
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< 1.8 nm (FWHM @Hg 253.7 nm)
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Detection Limit
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2 ng/L (dsDNA)
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Maximum Concentration
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15,000 ng/L (dsDNA)
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Measurement Time
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< 5 seconds
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Sample Pedestal Material
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303 stainless steel and quartz fiber
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Software Compatibility
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Windows XP and Vista (32 bit), Windows 7 Pro (32 and 64 bit), Windows 8 (32 and 64 bit), and Windows 10 Pro (64-bit)
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Dimensions
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5.5 x 7.9 in. (14 x 20 cm.)
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Weight
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4.4 lb. (2.0 kg.)
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Electrical Requirements
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12 VDC, 12 to 18 W (Max: 30 W)
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- Confirm the power supply and PCB output correct, stable voltages across all required connections
- Inspect the display for any dim or dead pixels; replace affected components to maintain full visual clarity and operational accuracy
- Verify full functionality of all buttons, ensuring precise and responsive input control
- Check the XY assembly for proper movement and alignment; thoroughly clean all railings, removing any debris or contaminants
- Confirm stable software communication and connection between the instrument and PC, addressing any connectivity issues
- Inspect and clean or replace all filters as required to ensure optimal airflow and performance
- Examine internal wiring and electrical connections for any breaks, disconnections, or tears; replace or secure any compromised wiring
- Test the lamp’s full functionality, replacing it if any performance issues, dimming, or faults are detected
- Execute the necessary validation protocol, documenting completion with a date on a calibration/verification certificate
- Perform and verify appropriate updates to the PC operating system for compatibility and security
- Conduct a thorough cosmetic inspection, ensuring all external components meet quality and aesthetic standards
Technician Inspection and Recertification Checklist: Instruments
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
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Task
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Completed?
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Remarks?
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Confirm stable, correct voltage outputs from power supply and PCB
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Completed
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Inspect display for dim or dead pixels; replace as needed
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Completed
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Verify functionality of all buttons for precise, responsive control
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Completed
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Check XY assembly for proper movement; clean railings to remove debris
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Completed
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Confirm stable software communication between instrument and PC
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Completed
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Inspect and clean or replace filters to maintain optimal performance
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Completed
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Examine internal wiring for damage; replace or secure compromised connections
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Completed
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Test lamp functionality; replace if dimming or faults are detected
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Completed
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Execute validation protocol and document on calibration/verification certificate
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Completed
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Update PC operating system for compatibility and security
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Completed
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Conduct cosmetic inspection to ensure quality of external components
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Completed
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To View More of Our Recertification Protocol Click Here
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