Absorbance Specifications
Light Source
Xenon flash lamp
Wavelength Selection
Monochromator
Wavelength Range
230 to 999 nm, in 1 nm increments
Bandpass
2 nm (230 to 285 nm), 4 nm (> 285 nm)
Dynamic Range
0 to 4.0 OD
Photometric Resolution
0.0001 OD
Pathlength Correction
Yes
Photometric Accuracy
< 1% at 2.0 OD typical
< 3% at 3.0 OD typical
Photometric Repeatability
< 0.5% at 2.0 OD typical
Fluorescence Intensity Specifications
Tungsten halogen lamp
Double grating monochromators (top/bottom) and deep blocking filters/dichroic mirrors (top)
Monochromators: 250 to 850 nm
Filters: 200 to 700 nm (850 nm option)
Monochromators: 5 decades
Filters/Mirrors: > 6 decades
Sensitivity
Top Monochromator: Fluorescein 2 pM typical (0.2 fmol/well 384-well plate)
Bottom Monochromator: Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate)
Filters/Mirrors: Fluorescein 1 pM typical (0.1 fmol/well 384-well plate)
Luminescence Specifications
300 to 700 nm
< 6 decades
10 amol ATP typical (flash)
Fluorescence Polarization Specifications
Tungsten halogen
Deep blocking filters/dichroic mirrors (top)
400 to 700 nm (320 to 850 nm option)
3 mP at 1 nm fluorescein typical
Time-Resolved Fluorescence Specifications
High energy xenon flash lamp
200 to 700 nm (850 nm option)
Europium 60 fM typical with filters (6 amol/well in 384-well plate)
UV/Vis Photometric Performance
200 - 1000 nm
Monochromator, tunable in 1.0 nm increments
Wavelength Bandwidth
°¤ 4.0 nm
Wavelength Accuracy
± 2.0 nm
Wavelength Repeatability
± 0.2 nm
Photometric Range
0 - 4.0 OD
0.001 OD
Photometric Accuracy (Microplate)
< ± 0.006 OD ± 1.0%, 0-2 OD
Photometric Precision
< ± 0.003 OD ± 1.0%, 0-2 OD
Baseline Flatness
< 0.001 OD
Stray Light
< 0.05% @ 230 nm
Fluorescence Photometric:
Dual Monochromators
1 nm increments EX 250 - 850 nmEM 360 - 850 nm
Bandwidth (EX, EM)
9, 9 nm
Detection Limit
3.0 fmol/well FITC 200µl in 96 wells
Time Resolved Fluorescence:
360 - 850 nm
0.5 fmol/well Eu-chelate (obtained with DELFIA reagent from PE by using a 384 well plate)
Data Collection
50 - 1450 µsec, 200µsec increments
Luminescnece:
10 amol/well alkaline phosphatase 200 µl/well (obtained with Emerald II reagent from ABI)
General Specifications:
Xenon Flash Lamp
Detector
Photomultiplier (R-3896)
Plate Formats
6, 12, 24, 48, 96, 384 wells
Read Time
96 well (Abs 18 sec, FI 15 sec) 384 well (Abs 49 sec, FI 45 sec)
Shaker Time
0 to 999 seconds
Temperature Range
Ambient +4 °C to 45°C
Temperature Accuracy
± 1 °C @ 37°C
Temperature Uniformity
± 0.5°C @ 37°C set point
Power
110 - 120 Vac 50/60 Hz
Absorbance Photometric Performance
Monochromator, tunable in 1 nm increments
200 to 1000 nm
< ±0.006 OD ±1%, 0 to 2 OD
Photometric Accuracy (Cuvette)
< ±0.005 OD ±1%, 0 to 2 OD
< ±0.003 OD ±1%, 0 to 2 OD
Fluorescence Photometric Performance
1 nm increment selection
EX Wavelength Range
250 to 850 nm
EM Wavelength Range
Wavelength Bandwidth (EX, EM)
9 nm
Top-Read Optimized Sensitivity
5 pM (fluorescein) in 96 wells
Top-Read Guaranteed Sensitivity
15 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
360 to 850 nm
50 to 1450 µsec., 200 µsec. increments
Guaranteed Sensitivity
7 pM Eu-chelate
Luminescence (secondary mode)
50 fM alkaline phosphatase
General Photometric Performance
Xenon flash lamp (1 joule/flash)
Photomultiplier tube (PMT)
96 wells: Abs 18 sec., Fl 15 sec.
384 wells: Abs 49 sec., Fl 45 sec.
Temperature Control
Ambient +4°C to 45°C
< 1°C at 37°C set point
±1°C at 37°C set point
UV/Vis Photometric Performance:
200 ‚ 1000 nm
0 ‚ 4.0 OD
1 nm increments
EX 250 ‚ 850 nm
EM 360 ‚ 850 nm
Absorbance Accuracy
± 2% (at 0.76 absorbance at 257 nm)
Absorbance Range
0.02 to 300 absorbance (10 mm equivalent)
Absorbance Precision
0.002 absorbance (1 mm path)
Minimum Sample Size
0.5µl
Pathlength
1 mm (auto-ranging to 0.05 mm)
Detector Type
2048-element linear silicon CCD array
190 to 840 nm
± 1 nm
Spectral Resolution
< 1.8 nm (FWHM @Hg 253.7 nm)
2 ng/µL (dsDNA)
Maximum Concentration
15,000 ng/µL (dsDNA)
Measurement Time
< 5 seconds
Sample Pedestal Material
303 stainless steel and quartz fiber
Software Compatibility
Windows XP and Vista (32 bit), Windows 7 Pro (32 and 64 bit), Windows 8 (32 and 64 bit), and Windows 10 Pro (64-bit)
Dimensions
5.5 x 7.9 in. (14 x 20 cm.)
Weight
4.4 lb. (2.0 kg.)
12 VDC, 12 to 18 W (Max: 30 W)
4 ng/L (dsDNA)
0.12 mg/mL (BSA)
1,500 ng/L (dsDNA)
45 mg/mL (BSA)
Deuterium: 190 nm to approximately 800 nm
Tungsten: 370 nm to 1100 nm
Within ± 0.005 AU at 440, 465, 546.1, 590, and 635 nm at approximately 1 AU using the NIST-traceable 930e
± 0.01 AU at 235, 257, 313, 350 nm using a 6 % w/v potassium dichromate NIST-traceable 935a solution in 0.01 N sulfuric acid
> 0.03% at 340 nm measured with 50 g/l NaNO2 solution
> 0.05% at 220 nm measured with 10 g/l NaI solution
> 1% at 198 nm measured with 1.2% KCl solution
