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Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader
Detection Modes
Abs (UV-Vis), FI, TRF, FP, Lumi
Microplate Formats
6-,12-,24-,48-,96-, 384-wells
Read Types
Endpoint, Kinetic, Spectrum, Well Scan
- Multi-mode plate reader capable of UV-Visible Absorbance, Fluorescence Intensity, Time-Resolved Fluorescence, Fluorescence Polarization, and Luminescence
- Dual monochromator optics allowing a wide range of bioresearch and drug discovery applications without filter changes
- Suitable for various applications including ELISA, nucleic acid, protein assays, microbial growth, and more
- Features a Xenon Flash Lamp light source and Photomultiplier (R-3896) detector for high sensitivity and accuracy
- Compatible with multiple plate formats from 6 to 384 wells, providing flexibility for different assay requirements
- Includes temperature control from Ambient +4°C to 45°C with uniformity of ± 0.5°C at 37°C set point
- Features plate shaking with a timer from 0 to 999 seconds
The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. The SpectraMax M5 possesses five preferred modes for multiple detection benefits in one platform: UV-Visible Absorbance (Abs), Fluorescence Intensity (FI), Time-Resolved Fluorescence (TRF), Fluorescence Polarization (FP), Luminescence (Lumi).
Applications include endpoint, kinetic, spectrum, and multi-point well-scanning combining absorbance and fluorescence in 6-well to 384-well microplates, as well as endpoint, kinetic, and spectrum applications in absorbance and fluorescence using cuvettes, can be run with little to no optimization. For use within biochemistry, cell biology, immunology, molecular biology, and microbiology. Typical applications include ELISA, nucleic acid, protein, enzymatic type homogenous and heterogeneous assays, microbial growth, endotoxin testing, and pipettor calibration.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader includes:
- > Windows 10 Data Station Pre-Loaded with SoftMax Pro Software 6.3
- > Interface Cable
- > Molecular Devices Absorbance and Fluorescence Validation Report
- > Top Read Plate Adapter
- > Operator's Guide
- > Power Cord
- > Full Parts and Labor 180 Day Warranty
Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications
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UV/Vis Photometric Performance:
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Wavelength Range
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200 to 1000 nm
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Wavelength Selection
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Monochromator, tunable in 1.0 nm increments
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Wavelength Bandwidth
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≤ 4.0 nm
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Wavelength Accuracy
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± 2.0 nm
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Wavelength Repeatability
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± 0.2 nm
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Photometric Range
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0 to 4.0 OD
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Photometric Resolution
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0.001 OD
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Photometric Accuracy (Microplate)
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< ± 0.006 OD ± 1.0%, 0-2 OD
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Photometric Precision
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< ± 0.003 OD ± 1.0%, 0-2 OD
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Baseline Flatness
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< 0.001 OD
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Stray Light
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< 0.05% @ 230 nm
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Fluorescence Photometric:
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Dual Monochromators
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1 nm increments
EX: 250 to 850 nm
EM: 360 to 850 nm
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Bandwidth (EX, EM)
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9, 15 nm
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Detection Limit
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3.0 fmol/well FITC 200ul in 96 wells
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Time Resolved Fluorescence:
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Wavelength Range
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360 to 850 nm
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Sensitivity
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0.5fmol/well Eu-chelate (obtained with DELFIA reagent from PE by using a 384 well plate)
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Data Collection
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50 to 1450 usec increments
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Luminescence:
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Wavelength Range
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360 to 850 nm
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Detection Limit
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10 amol/well alkaline phosphatase 200 ul/well (obtained with Emerald II reagent from ABI)
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General Specifications:
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Light Source
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Xenon Flash Lamp
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Detector
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Photomultiplier (R-3896)
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Plate Formats
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6, 12, 24 , 48, 96, 384 wells
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Read Time
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96 well (Abs 18 sec, FI 15 sec)
384 well (Abs 49 sec, FI 45 sec)
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Shaker Time
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0 to 999 seconds
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Temperature Range
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Ambient +4C to 45C
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Temperature Uniformity
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± 0.5C @ 37C set point
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Electrical
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110 to 120 VAC, 50/60Hz
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- Confirm the power supply and PCB output correct, stable voltages across all required connections
- Inspect the display for any dim or dead pixels; replace affected components to maintain full visual clarity and operational accuracy
- Verify full functionality of all buttons, ensuring precise and responsive input control
- Check the XY assembly for proper movement and alignment; thoroughly clean all railings, removing any debris or contaminants
- Confirm stable software communication and connection between the instrument and PC, addressing any connectivity issues
- Inspect and clean or replace all filters as required to ensure optimal airflow and performance
- Examine internal wiring and electrical connections for any breaks, disconnections, or tears; replace or secure any compromised wiring
- Test the lamp’s full functionality, replacing it if any performance issues, dimming, or faults are detected
- Execute the necessary validation protocol, documenting completion with a date on a calibration/verification certificate
- Perform and verify appropriate updates to the PC operating system for compatibility and security
- Conduct a thorough cosmetic inspection, ensuring all external components meet quality and aesthetic standards
Technician Inspection and Recertification Checklist: Instruments
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
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Task
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Completed?
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Remarks?
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Confirm stable, correct voltage outputs from power supply and PCB
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Completed
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Inspect display for dim or dead pixels; replace as needed
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Completed
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Verify functionality of all buttons for precise, responsive control
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Completed
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Check XY assembly for proper movement; clean railings to remove debris
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Completed
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Confirm stable software communication between instrument and PC
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Completed
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Inspect and clean or replace filters to maintain optimal performance
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Completed
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Examine internal wiring for damage; replace or secure compromised connections
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Completed
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Test lamp functionality; replace if dimming or faults are detected
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Completed
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Execute validation protocol and document on calibration/verification certificate
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Completed
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Update PC operating system for compatibility and security
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Completed
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Conduct cosmetic inspection to ensure quality of external components
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Completed
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To View More of Our Recertification Protocol Click Here
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