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Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader
Wavelength Range
200 to 1000 nm
Wavelength Bandwidth
≤ 4.0 nm full width half maximum
Wavelength Accuracy
± 2.0 nm across wavelength range
- Supports absorbance (UV-Vis), fluorescence intensity, and luminescence detection modes
- Dual monochromators for precise wavelength selection with 1 nm increments
- Top-read and bottom-read modes for enhanced sensitivity in solution and cell-based assays
- PathCheck technology ensures accurate pathlength correction for absorbance measurements
- Integrated temperature control from ambient +2°C to 60°C with ±1°C accuracy
- Dedicated luminescence photomultiplier tube (PMT) for low background noise
- Compatible with 6-, 12-, 24-, 48-, 96-, and 384-well microplates
- Robotics-compatible design for automation in high-throughput applications
- Xenon flash lamp for reliable and consistent light source performance
- Shaker with programmable timing from 0 to 999 seconds
The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection
microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have optical performance comparable to a
top-of-the-line dedicated spectrofluorometer or spectrophotometer. Endpoint,
kinetic, spectrum and area-well scanning read types and PathCheck allow
homogeneous and heterogeneous microplate assays to be performed in this
flexible system. This FI, Abs, and Lumi microplate reader with a built-in cuvette port
allows for easy conversion and optimization of very-low-throughput assays into
medium-high-throughput microplate assays.
The SpectraMax M3 allows faster, more precise results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. The SpectraMax M3 features top-read and bottom-read modes, resulting in greater sensitivity for solution and cell-based assays. Within endpoint and kinetic measurement protocols, up to four wavelength pairs can be read at a time, allowing for improved efficiency for FRET assays. For Luminescence, the SpectraMax M3 utilizes a dedicated luminescence photomultiplier tube (PMT), providing the maximum signal and lowest background noise as possible for glow luminescence report gene assays. Key Features: dual mode cuvette port, robotics compatible, multilabel, dual monochromators, and PathCheck.
Applications include: DNA, RNA, and protein quantitation and purity; PicoGreen, NanoOrange, and Bradford; ELISAs and enzyme kinetics; drug dissolution profiles; live or dead viability or cytotoxicity assays; Caspase-3 and protease assays; cAMP assays using CatchPoint Assay Kits; and low volume applications.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader includes:
- > Windows 10 Data Station Pre-Loaded with SoftMax Pro Software 6.3
- > Interface Cable
- > Molecular Devices Absorbance and Fluorescence Validation Report
- > Top Read Plate
- > Power Cord
- > Operator's Guide
- > Full 180 Day Parts and Labor Warranty
Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Specifications
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Absorbance Photometric Performance
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Wavelength Selection
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Monochromator, tunable in 1 nm increments
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Wavelength Range
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200 to 1000 nm
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Wavelength Bandwidth
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≤ 4.0 nm full width half maximum
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Wavelength Accuracy
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± 2.0 nm across wavelength range
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Wavelength Repeatability
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± 0.2 nm
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Photometric Range
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0.000 to 4.000 OD
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Photometric Resolution
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0.001 OD
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Photometric Accuracy (Microplate)
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< ± 0.006 OD ± 1%, 0 to 2 OD
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Photometric Accuracy (Cuvette)
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< ± 0.005 OD ± 1%, 0 to 2 OD
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Photometric Precision
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< ± 0.003 OD ± 1%, 0 to 2 OD
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Baseline Flatness
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< 0.001 OD
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Stray Light
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< 0.05% @ 230 nm
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Fluorescence Intensity Performance
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Dual Monochromators
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1 nm increment selection
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EX Wavelength Range
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250 to 850 nm
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EM Wavelength Range
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250 to 850 nm
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Wavelength Bandwidth (EX, EM)
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9 nm
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Top-Read Optimized Sensitivity
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≤ 1 pM (fluorescein) in 96 wells
≤ 1.5 pM (fluorescein) in 384 wells
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Top-Read Guaranteed Sensitivity
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< 5 pM (fluorescein) in 96 wells or cuvette
< 20 pM in 384 wells
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Luminescence Photometric Performance
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Sensitivity
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< 2 fg/well for firefly luciferase in 96- and 384-well microplates (top read)
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Wavelength Range
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250 to 850 nm
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Crosstalk
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< 0.5% in 96- and 384- well microplates
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Dynamic Range
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Optimized Sensitivity
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≤ 43 pM ATP in 96 wells
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Guaranteed Sensitivity
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≤ 75 pM ATP in 96 wells
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General Photometric Performance
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Plate Formats
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6, 12, 24, 48, 96, 384 wells
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Light Source
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Xenon flash lamp (1 joule/flash)
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Detectors
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2 Photomultiplier tubes (PMT)
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Read Time
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96 wells: Abs 18 sec., Fl 17 sec., Lumi 120 sec.
384 wells: Abs 49 sec., Fl 48 sec., Lumi 420 sec.
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Shaker Time
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0 to 999 seconds
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Temperature Control
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Ambient +2°C to 60°C
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Temperature Uniformity
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< 1°C at 37°C set point
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Temperature Accuracy
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± 1°C at 37°C set point
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General Specifications
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Dimensions (H x W x D)
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21.8 x 51.2 x 39 cm (8.6 x 22.5 x 15.4 in)
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Weight
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16.4 kg (36 lbs)
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Computer Specifications
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Model
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Hewlett-Packard
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Processor
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Intel Core i5-4300U CPU
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RAM
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8GB
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Hard Drive
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240GB Internal SSD
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Operating System
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Windows 10 Professional, 64-bit
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- Confirm the power supply and PCB output correct, stable voltages across all required connections
- Inspect the display for any dim or dead pixels; replace affected components to maintain full visual clarity and operational accuracy
- Verify full functionality of all buttons, ensuring precise and responsive input control
- Check the XY assembly for proper movement and alignment; thoroughly clean all railings, removing any debris or contaminants
- Confirm stable software communication and connection between the instrument and PC, addressing any connectivity issues
- Inspect and clean or replace all filters as required to ensure optimal airflow and performance
- Examine internal wiring and electrical connections for any breaks, disconnections, or tears; replace or secure any compromised wiring
- Test the lamp’s full functionality, replacing it if any performance issues, dimming, or faults are detected
- Execute the necessary validation protocol, documenting completion with a date on a calibration/verification certificate
- Perform and verify appropriate updates to the PC operating system for compatibility and security
- Conduct a thorough cosmetic inspection, ensuring all external components meet quality and aesthetic standards
Technician Inspection and Recertification Checklist: Instruments
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
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Task
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Completed?
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Remarks?
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Confirm stable, correct voltage outputs from power supply and PCB
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Completed
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Inspect display for dim or dead pixels; replace as needed
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Completed
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Verify functionality of all buttons for precise, responsive control
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Completed
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Check XY assembly for proper movement; clean railings to remove debris
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Completed
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Confirm stable software communication between instrument and PC
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Completed
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Inspect and clean or replace filters to maintain optimal performance
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Completed
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Examine internal wiring for damage; replace or secure compromised connections
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Completed
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Test lamp functionality; replace if dimming or faults are detected
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Completed
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Execute validation protocol and document on calibration/verification certificate
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Completed
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Update PC operating system for compatibility and security
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Completed
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Conduct cosmetic inspection to ensure quality of external components
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Completed
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To View More of Our Recertification Protocol Click Here
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