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Molecular Devices SpectraMax M2e Multilabel Microplate Reader
The SpectraMax M2e is a dual-monochromator, multi-detection
microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading
capability. Detection modalities include absorbance (UV-Vis Abs) and
fluorescence intensity (FI) and have optical performance comparable to a
top-of-the-line dedicated spectrofluorometer or spectrophotometer. Endpoint,
kinetic, spectrum and area-well scanning read types and PathCheck allow
homogeneous and heterogeneous microplate assays to be performed in this
flexible system. The FI and Abs microplate reader with built-in cuvette port
allows easy conversion and optimization of very-low-throughput assays into
medium-high-throughput microplate assays.
The SpectraMax M2e allows faster, more precise results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. The M2e features top- and bottom-read modes, resulting in greater sensitivity for solution and cell-based assays. Within endpoint and kinetic measurement protocols, up to four wavelength pairs can be read at a time, allowing for improved efficiency for FRET assays. Key Features: dual mode cuvette port, robotics compatible, multilabel, dual monochromators, and PathCheck.
Applications include: DNA, RNA, and protein quantitation and purity; PicoGreen, NanoOrange, and Bradford; ELISAs and enzyme kinetics; drug dissolution profiles; live or dead viability or cytotoxicity assays; Caspase-3 and protease assays; cAMP assays using CatchPoint Assay Kits; and low volume applications.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The Molecular Devices SpectraMax M2e Multilabel Microplate Reader includes:
- > Windows 10 Data Station Pre-Loaded with SoftMax Pro Software 6.3
- > Interface Cable
- > Molecular Devices Absorbance and Fluorescence Validation Report
- > Top Read Plate
- > Power Cord
- > Operator's Guide
- > Full 180 Day Parts and Labor Warranty
Molecular Devices SpectraMax M2e Multilabel Microplate Reader Specifications
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Absorbance Photometric Performance
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Wavelength Selection
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Monochromator, tunable in 1 nm increments
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Wavelength Range
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200 to 1000 nm
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Wavelength Bandwidth
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? 4.0 nm
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Wavelength Accuracy
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± 2.0 nm
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Wavelength Repeatability
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± 0.2 nm
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Photometric Range
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0 to 4.0 OD
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Photometric Resolution
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0.001 OD
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Photometric Accuracy (Microplate)
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<
±0.006 OD ±1%, 0 to 2 OD
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Photometric Accuracy (Cuvette)
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<
±0.005 OD ±1%, 0 to 2 OD
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Photometric Precision
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<
±0.003 OD ±1%, 0 to 2 OD
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Baseline Flatness
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< 0.001 OD
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Stray Light
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< 0.05% @ 230 nm
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Fluorescence Photometric Performance
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Dual Monochromators
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1 nm increment selection
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EX Wavelength Range
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250 to 850 nm
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EM Wavelength Range
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250 to 850 nm
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Wavelength Bandwidth (EX, EM)
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9 nm
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Top-Read Optimized Sensitivity
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5 pM (fluorescein) in 96 wells
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Top-Read Guaranteed Sensitivity
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15 pM (fluorescein) in 96 wells
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Time-Resolved Fluorescence (secondary mode)
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Wavelength Range
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360 to 850 nm
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Data Collection
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50 to 1450
±sec., 200 ±sec. increments
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Guaranteed Sensitivity
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7 pM Eu-chelate
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Luminescence (secondary mode)
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Wavelength Range
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360 to 850 nm
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Guaranteed Sensitivity
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50 fM alkaline phosphatase
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General Photometric Performance
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Plate Formats
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6, 12, 24, 48, 96, 384 wells
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Light Source
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Xenon flash lamp (1 joule/flash)
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Detector
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Photomultiplier tube (PMT)
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Read Time
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96 wells: Abs 18 sec., Fl 15 sec.
384 wells: Abs 49 sec., Fl 45 sec.
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Shaker Time
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0 to 999 seconds
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Temperature Control
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Ambient +4
°C to 45 °C
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Temperature Uniformity
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< 1
°C at 37 °C set point
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Temperature Accuracy
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±1 °C at 37 °C set point
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General Specifications
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Dimensions (H x W x D)
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22 x 58 x 38 cm (8.6 x 22.8 x 15 in)
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Weight
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15.9 kg (25 lbs)
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Computer Specifications
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Model
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Hewlett-Packard
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Processor
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Intel Core i5-7200U CPU
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RAM
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16.0 GB
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Hard Drive
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240GB Internal SSD
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Operating System
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Windows 10 Professional, 64-bit
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- Confirm the power supply and PCB output correct, stable voltages across all required connections
- Inspect the display for any dim or dead pixels; replace affected components to maintain full visual clarity and operational accuracy
- Verify full functionality of all buttons, ensuring precise and responsive input control
- Check the XY assembly for proper movement and alignment; thoroughly clean all railings, removing any debris or contaminants
- Confirm stable software communication and connection between the instrument and PC, addressing any connectivity issues
- Inspect and clean or replace all filters as required to ensure optimal airflow and performance
- Examine internal wiring and electrical connections for any breaks, disconnections, or tears; replace or secure any compromised wiring
- Test the lamp’s full functionality, replacing it if any performance issues, dimming, or faults are detected
- Execute the necessary validation protocol, documenting completion with a date on a calibration/verification certificate
- Perform and verify appropriate updates to the PC operating system for compatibility and security
- Conduct a thorough cosmetic inspection, ensuring all external components meet quality and aesthetic standards
Technician Inspection and Recertification Checklist: Instruments
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
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Task
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Completed?
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Remarks?
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Confirm stable, correct voltage outputs from power supply and PCB
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Completed
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Inspect display for dim or dead pixels; replace as needed
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Completed
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Verify functionality of all buttons for precise, responsive control
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Completed
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Check XY assembly for proper movement; clean railings to remove debris
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Completed
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Confirm stable software communication between instrument and PC
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Completed
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Inspect and clean or replace filters to maintain optimal performance
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Completed
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Examine internal wiring for damage; replace or secure compromised connections
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Completed
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Test lamp functionality; replace if dimming or faults are detected
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Completed
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Execute validation protocol and document on calibration/verification certificate
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Completed
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Update PC operating system for compatibility and security
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Completed
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Conduct cosmetic inspection to ensure quality of external components
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Completed
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To View More of Our Recertification Protocol Click Here
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Meets Expectations 
