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Molecular Devices SpectraMax 384 Plus Microplate Reader
Photometric Range
0.0 to 4.0 OD
Wavelength Range
190 to 1000 nm
Wavelength Bandwidth
2 nm
- Integrates the precision of a UV/Vis spectrophotometer with a microplate reader for fast and reliable data reporting.
- Accommodates a wide range of sample types to ensure the needs of your laboratory are met.
- Offers endpoint, kinetic, and spectral scan modes for versatile data analysis.
- Revolutionizes microplate reading by normalizing well absorbance and correcting pipetting variations.
- Accurately measures liquid depths and volumes down to 5µl in each microplate well.
- Built-in linear and orbital shaking options for thorough sample mixing.
The Molecular Devices SpectraMax 384 Plus is a UV/Vis
spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate
drawer. The instrument reads any standard cuvette, microcuvette, test tube or
96- and 384-well standard or UV transparent microplate over a full 190 to 1000 nm
wavelength range. This 384 Plus comes from a long history of reliable tools
built by Molecular Devices. This robust system is capable of reading 96 or 384
wells, each with its own sample beam and reference detector, for optical density measurement at a selected wavelength with endpoint (for a single point in time), kinetic (over a specific period of time), or spectral scan mode (over a specific wavelength range).
The high-precision nine channel system (8 for the microplate; 1 for the cuvette port) consists of
sample beams and reference beams that mimic a dual beam spectrophotometer. The filter-less system includes monochromator, allowing for up to 6 wavelengths within the visible spectrum at a time, saving time without the need to change out filters.
The PathCheck sensor eliminates optical pathlength as a factor (and corrects
pipetting variation), closing the gap between microplate readers and
spectrophotometers. This is accomplished by PathCheck, in conjunction with the SoftMax Pro software, automatically normalizing well absorbance, allowing for the elimination of standard curves, and correcting volume differences in the wells. The 384 Plus measures liquid depth and volumes down to 5ul in
each microplate well and scales the microplate data to standard 1 cm cuvette
values. The monochromator selects wavelengths in 1nm increments, and the system is equipped
with a built-in programmable linear and orbital plate shaking device.
Key applications include: DNA and RNA analysis; ELISAs and immunoassays; cell viability, proliferation, and cytotoxicity; endpoint analysis; enzyme kinetic assays; protein quantitation; endotoxin detection; and PathCheck.
The Molecular Devices SpectraMax 384 Plus Microplate Reader includes:
Windows 10 Data Station with Pre-Loaded Softmax Pro Software 6.3
- > Interface Cable
- > Molecular Devices Absorbance Validation Report
- > Operator's Guide
- > Power Cord
- > Full 180 Day Parts and Labor Warranty
Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications
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Spectral Range
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190-1000 nm, tunable in 1 nm increments
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Wavelength Range
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190-1000 nm
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Wavelength Selection
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Monochromator, tunable 1.0nm increments
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Speed Read Mode
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Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds
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Wavelength Bandwidth
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2 nm
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Wavelength Accuracy
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< ± 1.0 nm
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Wavelength Repeatability
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± 0.2 nm
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Photometric Range
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0.0 to 4.0 OD
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Photometric Resolution
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0.001 OD
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Photometric Accuracy (plate)
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< ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
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Photometric Accuracy (cuvette)
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< ± 0.005 OD ± 1.0%, 0.0 - 2.0 OD
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Photometric Precision
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< ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
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Photometric Baseline Flatness
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< 0.001 OD
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Stray Light
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< 0.05% @ 230 nm
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Light Source
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Xenon Flash Lamp
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Temperature Range
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Ambient + 4C up to 45C
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Temperature Uniformity (plate)
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± 0.5C @ 37C
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Dimensions
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22 cm (h) x 58 cm (w) x 38 cm (d)
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Weight
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30lbs
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Photometric Analysis Modes
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Standalone
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Single wavelength absorbance or %Transmittance reading of the cuvette
(or test tube)
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Using SoftMax Pro
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Express data as Absorbance or %Transmittance
Single wavelength reading of microplate and/or cuvette
Multiple wavelength (up to six) reading of microplate and/
or cuvette
Kinetic and kinetic graphics of microplate and/or cuvette
Spectral scan (190 to 1000 nm) of microplate and/or cuvette
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Measurement Time (calibration off)
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Read Time (endpoint) Standard Read Microplate
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96 wells in 9 seconds (single wavelength)
96 wells in 9*N seconds (N wavelengths)
384 wells in 29 seconds (single wavelength)
384 wells in 29*N seconds (N wavelengths)
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Read Time (endpoint) Speed Read Microplate
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96 wells in 5 seconds (single wavelength)
96 wells in 5*N seconds (N wavelengths)
384 wells in 16 seconds (single wavelength)
384 wells in 16*N seconds (N wavelengths)
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Kinetic Read Intervals Microplate
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96 wells, 9-second minimum interval between readings (single
wavelength)
1 column, 2-second minimum interval between readings (single
wavelength)
384 wells, 29-second minimum interval between readings (single
wavelength)
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Read Time Cuvette
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1 second (single wavelength)
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Kinetic Read Intervals Cuvette
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2-second minimum interval between readings
(single wavelength)
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Scan Speed
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Cuvette: Normal Scan
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45*K nm/min (K = wavelength interval)
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Cuvette: Speed Scan
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130*K nm/min
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Microplate: Normal Scan
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33*K nm/min (8-well strip); 21*K nm/min (16-well strip)
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Microplate: Speed Scan
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135*K nm/min (8-well strip); 77*K nm/min (16-well strip)
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Temperature Regulation
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Reading Chamber
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Isothermal when temperature regulation is not enabled
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Range
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4°C above ambient to 45°C when temperature regulation enabled.
The ambient temperature must be >20°C to achieve temperature regulation at 45°C.
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Resolution
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± 0.1°C
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Accuracy
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± 1.0°C for microplate and cuvette chamber
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Temperature Uniformity at Equilibrium
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± 0.5°C at 37°C
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Chamber Warm-Up Time
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15 to 30 minutes (measured on air) after initiation of temperature regulation
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Drift
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± 0.2°C (regulated)
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Computer Specifications
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Model
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Hewlett-Packard
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Processor
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Intel Core i5-7200U CPU
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RAM
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16.0 GB
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Hard Drive
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240GB Internal SSD
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Operating System
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Windows 10 Professional, 64-bit
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- Confirm the power supply and PCB output correct, stable voltages across all required connections
- Inspect the display for any dim or dead pixels; replace affected components to maintain full visual clarity and operational accuracy
- Verify full functionality of all buttons, ensuring precise and responsive input control
- Check the XY assembly for proper movement and alignment; thoroughly clean all railings, removing any debris or contaminants
- Confirm stable software communication and connection between the instrument and PC, addressing any connectivity issues
- Inspect and clean or replace all filters as required to ensure optimal airflow and performance
- Examine internal wiring and electrical connections for any breaks, disconnections, or tears; replace or secure any compromised wiring
- Test the lamp’s full functionality, replacing it if any performance issues, dimming, or faults are detected
- Execute the necessary validation protocol, documenting completion with a date on a calibration/verification certificate
- Perform and verify appropriate updates to the PC operating system for compatibility and security
- Conduct a thorough cosmetic inspection, ensuring all external components meet quality and aesthetic standards
Technician Inspection and Recertification Checklist: Instruments
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
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Task
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Completed?
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Remarks?
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Confirm stable, correct voltage outputs from power supply and PCB
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Completed
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Inspect display for dim or dead pixels; replace as needed
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Completed
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Verify functionality of all buttons for precise, responsive control
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Completed
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Check XY assembly for proper movement; clean railings to remove debris
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Completed
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Confirm stable software communication between instrument and PC
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Completed
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Inspect and clean or replace filters to maintain optimal performance
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Completed
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Examine internal wiring for damage; replace or secure compromised connections
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Completed
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Test lamp functionality; replace if dimming or faults are detected
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Completed
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Execute validation protocol and document on calibration/verification certificate
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Completed
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Update PC operating system for compatibility and security
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Completed
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Conduct cosmetic inspection to ensure quality of external components
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Completed
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To View More of Our Recertification Protocol Click Here
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