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Molecular Devices Spectramax Gemini EM Fluorescence Microplate Reader
Microplate Formats 6-,12-,24-,48-,96-, 384-wells
Wavelength Range (Excitation/Emission) 250 to 850 nm
Read Types Endpoint, Kinetic, Spectrum, Well Scan
- Built on the Gemini platform, offering high-quality top and bottom reading for solution and cell-based assays
- Versatile format compatibility, ranging from 6- to 384-well microplates, with various modes like endpoint, spectral scan, and kinetic
- Dual monochromators for excitation and emission with a wavelength range from 250 to 850 nm
- Capable of reading up to four wavelength pairs in endpoint and kinetic measurements
- Features AutoPMT electronics for superb accuracy and reproducibility of results
- Suitable for a wide range of applications including enzyme assays, protein quantitation, ELISAs, and cell viability tests
- Quick microplate read time with 96 wells in 15 seconds and 384 wells in 45 seconds
- Temperature control from ambient +4°C up to 45°C with uniformity within 1°C at 37°C
The Molecular Devices Spectramax Gemini EM Microplate Reader is a highly versatile and sophisticated instrument designed for a broad spectrum of applications ranging from enzyme assays to cell viability tests. This state-of-the-art reader is part of the Gemini platform, known for delivering top-notch performance in both solution and cell-based assays. It supports a wide array of microplate formats, accommodating 6- to 384-well plates, and offers various read types including endpoint, kinetic, spectrum, and well scan modes. Featuring dual monochromators for excitation and emission, the Gemini EM boasts a broad wavelength range from 250 to 850 nm, allowing for up to four wavelength pairs in endpoint and kinetic measurements.
The Gemini EM’s advanced AutoPMT electronics ensure exceptional accuracy and reproducibility, while the reader's rapid throughput capabilities are evident in its ability to process 96 wells in just 15 seconds and 384 wells in 45 seconds. Additionally, the device offers precise temperature control, ranging from Ambient +4°C to 45°C, with uniformity within 1°C at 37°C, ensuring optimal conditions for various assays. The Gemini EM is not only fast and reliable but also designed for superior sensitivity, with top and bottom read sensitivities of 3.0 fmol/well and 8.0 fmol/well for FITC in 96 wells, respectively. Its dynamic range spans six orders of magnitude, accommodating a wide range of assay requirements. With its comprehensive suite of features, the Spectramax Gemini EM Microplate Reader is an essential tool for researchers demanding high quality, flexibility, and efficiency in their laboratory workflows.
Applications for the Gemini EM include enzyme assays, protein quantitation, microbial growth, ELISAs and immunoassays, transporter assays, nucleic acid quantitation, phosphotases/kinases, reporter gene assays, and cell viability, proliferation, and cytotoxicity.
The Molecular Devices Spectramax Gemini EM Microplate Reader includes:
- > Windows 10 Data Station with Pre-Loaded Softmax Pro Software 6.3
- > Operator's Manual
- > Interface Cable
- > Molecular Devices Fluorescence Validation Report
- > Full 180 Day Part and Labor Warranty
Molecular Devices Gemini EM Specifications
General Info
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Dimensions (H x W x D)
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8.6 x 22.8 x 15 in (22 x 58 x 38 cm)
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Weight
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35 lbs (16 kg)
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Power Source
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100-240 VAC, 50/60 Hz
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Performance
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Wavelength Range Excitation
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250 to 850 nm
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Wavelength Range Emission
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250 to 850 nm
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Wavelength Selections
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Monochromator, tunable 1.0 nm increments
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Wavelength Bandwidth
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9 nm
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Wavelength
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< 2.0 nm
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Top Read Sensitivity
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3.0 fmol/well FITC 200 l in 96 wells
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Bottom Read Sensitivity
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8.0 fmol/well FITC 200 l in 96 wells
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Light Source
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Xenon flash lamp
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Microplate Read Time
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Normal Mode
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96 wells in 15 seconds
384 wells in 45 seconds
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Dynamic Range
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6 orders of magnitude
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Temperature Range
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Ambient + 4 up to 45C
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Temperature Uniformity
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1 at 37C
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Computer Specifications
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Processor
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Intel I5-7200U CPU
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Ram
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16.0 GB
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Brand
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Hewlett-Packard
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Hard drive
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240GB Solid State Drive
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Operating System
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Windows 10 64-Bit
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- Confirm the power supply and PCB output correct, stable voltages across all required connections
- Inspect the display for any dim or dead pixels; replace affected components to maintain full visual clarity and operational accuracy
- Verify full functionality of all buttons, ensuring precise and responsive input control
- Check the XY assembly for proper movement and alignment; thoroughly clean all railings, removing any debris or contaminants
- Confirm stable software communication and connection between the instrument and PC, addressing any connectivity issues
- Inspect and clean or replace all filters as required to ensure optimal airflow and performance
- Examine internal wiring and electrical connections for any breaks, disconnections, or tears; replace or secure any compromised wiring
- Test the lamp’s full functionality, replacing it if any performance issues, dimming, or faults are detected
- Execute the necessary validation protocol, documenting completion with a date on a calibration/verification certificate
- Perform and verify appropriate updates to the PC operating system for compatibility and security
- Conduct a thorough cosmetic inspection, ensuring all external components meet quality and aesthetic standards
Technician Inspection and Recertification Checklist: Instruments
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
Task
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Completed?
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Remarks?
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Confirm stable, correct voltage outputs from power supply and PCB
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Completed
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Inspect display for dim or dead pixels; replace as needed
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Completed
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Verify functionality of all buttons for precise, responsive control
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Completed
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Check XY assembly for proper movement; clean railings to remove debris
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Completed
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Confirm stable software communication between instrument and PC
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Completed
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Inspect and clean or replace filters to maintain optimal performance
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Completed
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Examine internal wiring for damage; replace or secure compromised connections
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Completed
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Test lamp functionality; replace if dimming or faults are detected
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Completed
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Execute validation protocol and document on calibration/verification certificate
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Completed
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Update PC operating system for compatibility and security
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Completed
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Conduct cosmetic inspection to ensure quality of external components
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Completed
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To View More of Our Recertification Protocol Click Here
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