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HP Agilent 1100 HPLC System with Variable Wavelength Detector & Fluorescence Detector
The HP Agilent 1100 HPLC System guarantees the most efficient mixing and pulse-free solvent delivery for consistently fast and accurate results. The system’s optimized design using a quaternary pump allows variable stroke volumes for stable solvent flow. This electronically activated inlet valve runs synchronous to the pistons to eliminate vapor formation in volatile solvent, making the HP Agilent 1100 both remarkably easy-to-use and maintenance-free. It also features a high-speed proportioning valve and a pre-configured degasser, which allow convenient low-pressure mixing and a large solvent choice flexibility. The HPLC System’s vacuum degasser ensures reduced operating costs without the use of helium and optimum instrument performance. The HP Agilent 1100 Variable Wavelength Detector lowers detection limits with simplified optics for high sensitivity, low baseline drift, and wide linear range. This wide linear range allows for reliable simultaneous quantitation of main and side products and impurities. The Agilent 1100 VWD’s minimum baseline noise gives it the highest baseline stability for the lowest detection limits. This VWD offers programmable wavelength switching for sensitivity and optimized selectivity optimized to each analyte’s elution, along with stop-flow wavelength scanning for quick wavelength optimization. The Agilent 1100 Fluorescence Detector brings high-sensitivity and selection through simultaneous multi-wavelength detection. This easy-to-use detector provides quantitative data and fluorescence spectra from a single run. The FD's spectral information can be used for rapid method optimization and verification of separation quality. The unit can be operated with a hand-held controller or computer running Clarity software. The HPLC System features extensive diagnostics with compatible data analysis software for on-site and remote trouble-shooting. All of our equipment is fully tested to perform at factory specifications before being shipped.
The HP Agilent 1100 HPLC System w/VWD & FLD Detectors includes:
- > G1311A Quat Pump (Binary Pump Available)
- > G1313A Autosampler (G1329A Thermal Autosampler and G1330B Thermal Unit available at an additional cost)
- > G1314A VWD Detector
- > G1321A FLD Detector
- > G1316A Column Compartment
- > G1322A Degasser (G1379A Degasser Available)
- > Windows 10 Professional 64-Bit Data Station with Flatscreen Monitor
- > Chemstation version 4.3 software installed
- > Can/Crossover Cables
- > Agilent G1369 Lan Interface Card
- > Solvent Tubing
- > 4 x Bottles, Caps and Solvent Filters
- > Stainless Steel Capillaries
- > 1/4" Wrench & Mobile Phase Priming Syringe
- > Waste Tubing
- > All Power Cords
- > Full 180 Day Parts and Labor Warranty
Below is a List of Diagnostic Tests Performed On This System
- Filter Test: The test moves the filter into the light path and measures the relative absorbance in the filter position.
- Dark Current Test: The test measures the dark current from the detector optic.
- Holmium Oxide Test: The test measures the Holmium spectrum from the built-in Holmium filter. The spectrum is evaluated for peaks at different wavelengths.
- Lamp Intensity Test: The test verifies functionality of the UV lamp at various wavelengths.
- Wavelength Intensity Test: The test confirms the accuracy of wavelengths measured.
- Baseline Noise Test: The test measures the amount of baseline noise drift identified by the detector.
- Pressure Test: The pressure test is a quick, built-in test designed to demonstrate the pressure tightness (up to 400 bar) of the system.
- Leak Test: The leak test is a built-in troubleshooting designed to demonstrate the leak-tightness of the pump.
- Thermostat Test: Evaluates the cooling and heating performance of the two Peltier elements.
- Injector Steps: Performs each movement of the sampling sequence under manual control. This is useful for confirming autosampler functionality.
- 4 Peak Test (Isocratic Standard): Standard test published by Agilent to confirm complete functionality of the 1100 HPLC System.
HP Agilent 1100 HPLC System Specifications
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Type
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Specification
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Detection type
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Double-beam photometer
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Light Source
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Deuterium lamp
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Wavelength Range
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190 - 600 nm
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Short term noise (ASTM) Single
and Multi-Wavelength
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± 0.75 × 10-5AU at 254 nm
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Drift
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3 × 10-4AU/hr at 254 nm
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Linear Absorbance range
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> 2 AU (5%) upper limit
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Wavelength accuracy
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± 1 nm
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Slit width
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1, 2, 4 , 8, 16 nm
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Band Width
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6.5 nm typical
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Diode Width
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< 1 nm
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Flow Cells
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Standard: 14-µl volume, 10-mm
cell path length and 40 bar
(588 psi) pressure maximum
High pressure: 14-µl volume,
10-mm cell path length and
400 bar (5880 psi) pressure
maximum
Micro: 1-µl volume, 5-mm cell
path length and 40 bar (588 psi)
pressure maximum
Semimicro: 5-µl volume, 6-mm
cell path length and 40 bar
(588 psi) pressure maximum
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Control and Data Evaluation
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Agilent ChemStation for LC
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Analog Outputs
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Recorder/integrator: 100 mV or 1 V,
output range 0.001 – 2 AU, two
outputs
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Communications
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Controller-area network (CAN), GPIB,
RS-232C, APG Remote: ready, start,
stop and shut-down signals, LAN
optional
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Safety and Maintenance
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Extensive diagnostics, error detection
and display (through control module
and ChemStation), leak detection, safe
leak handling, leak output signal for
shutdown of pumping system. Low
voltages in major maintenance areas.
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GLP Features
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Early maintenance feedback (EMF) for
continuous tracking of instrument
usage in terms of lamp burn time with
user-settable limits and feedback
messages. Electronic records of
maintenance and errors. Verification of
wavelength accuracy with built-in
holmium oxide filter.
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Housing
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All Materials recyclable
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Computer Specifications
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Model
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Dell Optiplex 7010 Desktop PC
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Processor
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Intel Core i5-3470 Processor 3.2GHz
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Ram
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16.0 GB
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Hard Drive
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250GB Internal SSD
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Operating System
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Windows 10 Professional, 64bit
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HP Agilent 1100 Series HPLC System Operator's Manual
 Click Here to View all the Technical Specs for the HP Agilent 1100 System
- Verify uniformity of main and residential firmware across all components, updating as necessary to ensure compatibility
- Confirm successful communication between software and the HPLC system, validating data transfer integrity
- Check the degasser’s internal vacuum to confirm it reaches the specified vacuum level for optimal performance
- Replace inlet, outlet, purge valve, and pump seals in the system pump to maintain reliable operation
- Ensure system pump passes pressure and leak tests, confirming secure and efficient performance
- Replace all capillary tubing with new OEM stainless steel tubing for durability and precision
- Remove autosampler transport assembly, replace X and Y bearings, and thoroughly clean carrier rails to remove debris and contaminants
- Replace metering valve seal, autosampler needle, and needle seat for consistent sampling accuracy
- Test column compartment switching valve functionality and replace its seal if necessary
- Confirm column compartment passes thermostat test to maintain precise temperature control
- Remove and clean internal detector optics for clear and accurate signal detection
- Replace UV and Vis detector lamps to ensure optimal light source stability and performance
- Verify flow cell absorbance meets manufacturer’s specifications for accurate measurement
- Confirm system response to isocratic test sample, ensuring stable and reproducible results
Technician Inspection and Recertification Checklist: HPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
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Task
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Completed?
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Remarks?
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Verify firmware uniformity and update as necessary
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Completed
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Confirm software communication with HPLC system
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Completed
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Check degasser vacuum level for optimal performance
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Completed
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Replace inlet, outlet, purge valve, and pump seals
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Completed
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Ensure pump passes pressure and leak tests
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Completed
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Replace all capillary tubing with OEM stainless steel tubing
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Completed
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Remove autosampler transport assembly, replace bearings, and clean rails
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Completed
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Replace metering valve seal, autosampler needle, and needle seat
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Completed
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Test column compartment switching valve and replace seal if needed
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Completed
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Confirm thermostat test in column compartment
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Completed
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Clean internal detector optics
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Completed
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Replace UV and Vis detector lamps
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Completed
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Verify flow cell absorbance meets manufacturer’s specifications
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Completed
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Confirm system response to isocratic test sample
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Completed
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To View More of Our Recertification Protocol Click Here
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