 |
 |
 |
|
GE AKTA Pure 25 M FPLC System
Flow Rate Range
0.001 to 25 ml/min
Maximum Operating Pressure
20 MPa (2900 psi)
Wavelength Range
190 to 700 nm
- Flexible and intuitive chromatography system suitable for proteins, peptides, and nucleic acid purification
- Features UNICORN software for seamless hardware, column, and resin integration
- Supports a broad range of research applications and large-scale preparative tasks
- Modular design offers extensive customization to meet specific purification requirements
- Flow rate ranges from 0.001 to 25 ml/min, extendable to 50 ml/min for column packing
- UV monitor with a wavelength range of 190 to 700 nm and absorbance range of -6 to 6 AU
- Conductivity monitor capable of measuring from 0.01 to 999.9 mS/cm
- pH monitor offers precise readings from 0 to 14 pH units, within a calibrated temperature range
- F9-C Fraction Collector allows up to 576 fractions, compatible with various vessel types
- Ensures high precision and reliability, drawing from over 50 years of protein research expertise
The GE AKTA Pure 25 M FPLC System represents a pinnacle of flexibility and accuracy in the purification of proteins, peptides, and nucleic acids, suitable for applications ranging from microgram to gram-level target production. This chromatography system integrates the intuitive UNICORN software, ensuring optimal interaction between its hardware, chromatography columns, and resins. Designed to thrive in a multiuser environment, the AKTA Pure 25 accommodates a diverse array of research applications and purification tasks.
The AKTA Pure 25 M system is notably modular, offering extensive customization options to tailor its capabilities to specific purification needs. Key specifications include a flow rate range of 0.001 to 25 ml/min, with the ability to extend up to 50 ml/min for column packing. It maintains flow rate precision with an RSD of less than 0.5% and a pressure range up to 20 MPa. The system's UV monitor delivers detailed monitoring with a wavelength range from 190 to 700 nm, absorbance range of -6 to 6 AU, and impressive linearity and low noise levels. It also features a conductivity monitor with a broad range from 0.01 to 999.9 mS/cm and a pH monitor capable of accurate readings from 0 to 14 pH units.
The addition of the F9-C Fraction Collector increases the system's functionality significantly, supporting up to 576 fractions and accommodating a variety of vessel types including deep well plates. Fraction volumes can be precisely controlled from 0.1 to 250 ml, ensuring adaptability and efficiency in sample collection. With more than 50 years of expertise in protein research and three decades in chromatography system development, the GE AKTA Pure 25 M FPLC System is a testament to GE's commitment to innovation and reliability in the field of protein purification. This system not only meets but anticipates the rigorous demands of modern laboratory workflows, making it an essential tool for researchers seeking high-purity results.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The GE AKTA Pure 25 M FPLC System includes:
- > P9 A System Pump A
- > P9 B System Pump B
- > U9-M UV Monitor
- > U9-D UV Detector
- > P9-S Sample Pump
- > C9 Conductivity Monitor
- > V9-pH Valve
- > V9-IS Sample Inlet Valve
- > V9-C Column Valve
- > V9-L Loop Valve
- > V9-Inj Injection Valve
- > V9-O Outlet Valve
- > V9-IAB Inlet Valve AB
- > M9 Mixer w/ 1.4ml Chamber
- > F9-C Fraction Collector
- > Windows 10 Professional 64-Bit Data Station with Installed UNICORN Version 7.1 Software
- > Installation Calibration Report
- > Communication Cables
- > Tubing, Power Cords
- > Full 180 Day Parts and Labor Warranty
GE AKTA Pure 25 M FPLC System Specifications
|
System Pump Specifications
|
|
Flow Rate Range
|
0.001 to 25 ml/min (up to 50 ml/min during column packing)
|
|
Flow Rate Accuracy
|
± 1.2%
|
|
Flow Rate Precision
|
RSD <0.5%
|
|
Pressure Range
|
0 to 20 MPa (2900 psi)
|
|
Pressure Limits
|
Programmable upper and lower
|
|
Viscosity Range
|
0.35 to 10 cP (5 cP above 12.5 ml.min)
|
|
UV Monitor Specifications
|
|
Wavelength Range
|
190 to 700 nm in steps of 1 nm, up to 3 wavelengths
|
|
Absorbance Range
|
-6 to 6 AU
|
|
Resolution
|
0.001 mAU
|
|
Wavelength Reproducibility
|
± 0.01 nm
|
|
Linearity
|
Within ± 2% at 0 to 2 AU
|
|
Drift
|
≤ | 0.2 mAU | AU/h (2 mm cell at 280 nm)
|
|
Noise
|
< 0.08 mAU
|
|
Operating Pressure
|
0 to 2 MPa
|
|
Lamp Operating Time
|
> 5000 hours
|
|
Flow Cell
|
Optical path length: 2 mm
Cell volume: 2 ul
Total volume: 11 ul
|
|
Conductivity Monitor Specifications
|
|
Conductivity Range
|
0.01 to 999.9 mS/cm
|
|
Accuracy
|
± 0.01 mS/cm or ± 2%, whichever is greater (within 0.3 to 300 mS/cm)
|
|
Operating Pressure
|
0 to 5 MPa
|
|
Flow Cell Volume
|
22 ul
|
|
Temperature Monitor Range
|
0°C to 99°C
|
|
Temperature Monitor Accuracy
|
± 1.5°C within 4°C to 45°C
|
|
pH Monitor Specifications
|
|
pH Reading Range
|
0 to 14
|
|
Accuracy
|
± 0.1 pH unit within pH 2 to 12, temperature within ± 3°C from calibration temperature
|
|
Operating Pressure
|
0 to 0.5 MPa
|
|
Flow Cell Volume
|
76 ul
|
|
F9-C Fraction Collector Specifications
|
|
Number of Fractions
|
Up to 576
|
|
Vessel Types
|
Deep well plates, 96, 48, or 24 wells
|
|
Fraction Volumes
|
0.1 to 250 ml
|
|
Spillage-Free Mode
|
Automatic, Drop Sync, or Accumulator
|
|
Flammable Liquids
|
No
|
|
Delay Volume (UV-Dispenser Head)
|
205 ul, 86 ul with optional tubing kit (i.d. 0.25 mm)
|
|
Dimensions (W x D x H)
|
15.4 x 23 x 12.6 (39 x 58.5 x 32 cm)
|
|
Weight
|
46.3 lb. (21 kg)
|
|
Computer Specifications
|
|
Model
|
Dell Optiplex 7050 Desktop PC
|
|
Processor
|
Intel Core i5-6500 Processor 3.6GHz
|
|
RAM
|
16GB
|
|
Hard Drive
|
250GB Internal SSD
|
|
Operating System
|
Windows 10 Professional, 64-bit
|
- Inspect all displays, replacing any dim or dead pixels to ensure optimal visual feedback
- Examine internal components of P-900, UV-900/UPC-900, and pH/C-900, replacing any malfunctioning parts to maintain performance
- Verify connections of all components via Uni-Net 1 & 2 interfaces for secure and reliable communication
- Check Uni-Net cables, replacing any faulty ones to ensure uninterrupted data transmission
- Inspect internal components of M-925, INV-907, PV-908, and PV-907 to confirm full operational functionality
- Replace all PEEK tubing with new OEM tubing and inspect all components within the flow path for potential clogs
- Confirm successful software interfacing with the FPLC system for seamless operation
- Replace pump piston seals, wash seals, and seal wash tubing, performing ultrasonic cleaning on each check valve in the pump flow path
- Calibrate pressure and flow rates of both the P-900 pump and P-960/950 pump (if applicable) for precise control
- Replace UV lamp and perform internal inspection tests to confirm complete functionality
- Install a new pH probe and verify the conductivity cell for accurate readings
- Calculate and adjust delay volume in software to reflect system configuration
- Clean or replace the drop sensor, accumulator wash, and height adjuster for consistent collection performance
- Verify fraction collector alignment and calibration with 15mL, 50mL, and microplate receptacles to ensure accuracy
- Run installation tests and thoroughly document all findings for comprehensive record-keeping
Technician Inspection and Recertification Checklist: FPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
|
Task
|
Completed?
|
Remarks?
|
|
Inspect all displays and replace dim/dead pixels
|
Completed
|
|
|
Examine internal components of P-900, UV-900/UPC-900, and pH/C-900
|
Completed
|
|
|
Verify connections via Uni-Net 1 & 2 interfaces
|
Completed
|
|
|
Check and replace any malfunctioning Uni-Net cables
|
Completed
|
|
|
Inspect internal components of M-925, INV-907, PV-908, PV-907
|
Completed
|
|
|
Replace PEEK tubing with OEM tubing and check for clogs
|
Completed
|
|
|
Confirm software interfacing with FPLC system
|
Completed
|
|
|
Replace pump seals and wash tubing; clean check valves
|
Completed
|
|
|
Calibrate pressure and flow rates of P-900 and P-960/950 pumps
|
Completed
|
|
|
Replace UV lamp and confirm full functionality
|
Completed
|
|
|
Install new pH probe and verify conductivity cell
|
Completed
|
|
|
Calculate delay volume and update in software
|
Completed
|
|
|
Clean or replace drop sensor, accumulator wash, and height adjuster
|
Completed
|
|
|
Verify fraction collector alignment with 15mL, 50mL, and microplate receptacles
|
Completed
|
|
|
Run installation tests and document all findings
|
Completed
|
|
To View More of Our Recertification Protocol Click Here
|
|
|
 |
|
 |
|
