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GE AKTA Pure 25 M FPLC System
GE AKTA Pure 25 M FPLC System
 
 
Our Price: $69,995.00

Condition: Fully Recertified and Tested to Perform at Manufacturer Specifications
Warranty: 180-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 6 to 8 Business Days
Included Documentation: Installation Calibration Report

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Quantity in Stock:1

Product Code: GE-AKP25M
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Description
Recertification Protocol
Technical Specification Sheet
 

GE AKTA Pure 25 M FPLC System



Flow Rate Range
0.001 to 25 ml/min

Maximum Operating Pressure
20 MPa (2900 psi)

Wavelength Range
190 to 700 nm

Features
  • Flexible and intuitive chromatography system suitable for proteins, peptides, and nucleic acid purification
  • Features UNICORN software for seamless hardware, column, and resin integration
  • Supports a broad range of research applications and large-scale preparative tasks
  • Modular design offers extensive customization to meet specific purification requirements
  • Flow rate ranges from 0.001 to 25 ml/min, extendable to 50 ml/min for column packing
  • UV monitor with a wavelength range of 190 to 700 nm and absorbance range of -6 to 6 AU
  • Conductivity monitor capable of measuring from 0.01 to 999.9 mS/cm
  • pH monitor offers precise readings from 0 to 14 pH units, within a calibrated temperature range
  • F9-C Fraction Collector allows up to 576 fractions, compatible with various vessel types
  • Ensures high precision and reliability, drawing from over 50 years of protein research expertise
The GE AKTA Pure 25 M FPLC System represents a pinnacle of flexibility and accuracy in the purification of proteins, peptides, and nucleic acids, suitable for applications ranging from microgram to gram-level target production. This chromatography system integrates the intuitive UNICORN software, ensuring optimal interaction between its hardware, chromatography columns, and resins. Designed to thrive in a multiuser environment, the AKTA Pure 25 accommodates a diverse array of research applications and purification tasks.

The AKTA Pure 25 M system is notably modular, offering extensive customization options to tailor its capabilities to specific purification needs. Key specifications include a flow rate range of 0.001 to 25 ml/min, with the ability to extend up to 50 ml/min for column packing. It maintains flow rate precision with an RSD of less than 0.5% and a pressure range up to 20 MPa. The system's UV monitor delivers detailed monitoring with a wavelength range from 190 to 700 nm, absorbance range of -6 to 6 AU, and impressive linearity and low noise levels. It also features a conductivity monitor with a broad range from 0.01 to 999.9 mS/cm and a pH monitor capable of accurate readings from 0 to 14 pH units.

The addition of the F9-C Fraction Collector increases the system's functionality significantly, supporting up to 576 fractions and accommodating a variety of vessel types including deep well plates. Fraction volumes can be precisely controlled from 0.1 to 250 ml, ensuring adaptability and efficiency in sample collection. With more than 50 years of expertise in protein research and three decades in chromatography system development, the GE AKTA Pure 25 M FPLC System is a testament to GE's commitment to innovation and reliability in the field of protein purification. This system not only meets but anticipates the rigorous demands of modern laboratory workflows, making it an essential tool for researchers seeking high-purity results.

All of our equipment is fully tested to perform at factory specifications before being shipped.

INCLUDED:
The GE AKTA Pure 25 M FPLC System includes:
  • > System Pump
  • > U9-M UV Monitor
  • > P9-S Sample Pump
  • > Conductivity Monitor
  • > pH Monitor
  • > F9-C Fraction Collector
  • > Windows 10 Professional 64-Bit Data Station with Installed UNICORN Version 7.1 Software
  • > Installation Calibration Report
  • > Communication Cables
  • > Tubing, Power Cords
  • > Full 180 Day Parts and Labor Warranty

GE AKTA Pure 25 M FPLC System Specifications

System Pump Specifications

Flow Rate Range

0.001 to 25 ml/min (up to 50 ml/min during column packing)

Flow Rate Accuracy

± 1.2%

Flow Rate Precision

RSD <0.5%

Pressure Range

0 to 20 MPa (2900 psi)

Pressure Limits

Programmable upper and lower

Viscosity Range

0.35 to 10 cP (5 cP above 12.5 ml.min)

UV Monitor Specifications

Wavelength Range

190 to 700 nm in steps of 1 nm, up to 3 wavelengths

Absorbance Range

-6 to 6 AU

Resolution

0.001 mAU

Wavelength Reproducibility

± 0.01 nm

Linearity

Within ± 2% at 0 to 2 AU

Drift

≤ | 0.2 mAU | AU/h (2 mm cell at 280 nm)

Noise

< 0.08 mAU

Operating Pressure

0 to 2 MPa

Lamp Operating Time

> 5000 hours

Flow Cell

Optical path length: 2 mm

Cell volume: 2 ul

Total volume: 11 ul

Conductivity Monitor Specifications

Conductivity Range

0.01 to 999.9 mS/cm

Accuracy

± 0.01 mS/cm or ± 2%, whichever is greater (within 0.3 to 300 mS/cm)

Operating Pressure

0 to 5 MPa

Flow Cell Volume

22 ul

Temperature Monitor Range

0°C to 99°C

Temperature Monitor Accuracy

± 1.5°C within 4°C to 45°C

pH Monitor Specifications

pH Reading Range

0 to 14

Accuracy

± 0.1 pH unit within pH 2 to 12, temperature within ± 3°C from calibration temperature

Operating Pressure

0 to 0.5 MPa

Flow Cell Volume

76 ul

F9-C Fraction Collector Specifications

Number of Fractions

Up to 576

Vessel Types

Deep well plates, 96, 48, or 24 wells

Fraction Volumes

0.1 to 250 ml

Spillage-Free Mode

Automatic, Drop Sync, or Accumulator

Flammable Liquids

No

Delay Volume (UV-Dispenser Head)

205 ul, 86 ul with optional tubing kit (i.d. 0.25 mm)

Dimensions (W x D x H)

15.4 x 23 x 12.6 (39 x 58.5 x 32 cm)

Weight

46.3 lb. (21 kg)

Computer Specifications

Model

Dell Optiplex 7050 Desktop PC

Processor

Intel Core i5-6500 Processor 3.6GHz

RAM

16GB

Hard Drive

250GB Internal SSD

Operating System

Windows 10 Professional, 64-bit

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