GE AKTA Pure 25 L FPLC System

The GE AKTA Pure 25 L FPLC System represents a pinnacle of flexibility and accuracy in the purification of proteins, peptides, and nucleic acids, suitable for applications ranging from microgram to gram-level target production. This chromatography system integrates the intuitive UNICORN software, ensuring optimal interaction between its hardware, chromatography columns, and resins. Designed to thrive in a multiuser environment, the AKTA Pure 25 accommodates a diverse array of research applications and purification tasks.

The AKTA Pure 25 L system is notably modular, offering extensive customization options to tailor its capabilities to specific purification needs. Key specifications include a flow rate range of 0.001 to 25 ml/min, with the ability to extend up to 50 ml/min for column packing. It maintains flow rate precision with an RSD of less than 0.5% and a pressure range up to 20 MPa. The system's UV monitor delivers detailed monitoring with a fixed wavelength of 280 nm, absorbance range of -6 to 6 AU, and impressive linearity and low noise levels. It also features a conductivity monitor with a broad range from 0.01 to 999.9 mS/cm and a pH monitor capable of accurate readings from 0 to 14 pH units.

The addition of the F9-C Fraction Collector increases the system's functionality significantly, supporting up to 576 fractions and accommodating a variety of vessel types including deep well plates. Fraction volumes can be precisely controlled from 0.1 to 250 ml, ensuring adaptability and efficiency in sample collection. With more than 50 years of expertise in protein research and three decades in chromatography system development, the GE AKTA Pure 25 L FPLC System is a testament to GE's commitment to innovation and reliability in the field of protein purification. This system not only meets but anticipates the rigorous demands of modern laboratory workflows, making it an essential tool for researchers seeking high-purity results.

All of our equipment is fully tested to perform at factory specifications before being shipped.

GE AKTA Pure 25 L FPLC System Specifications

System Pump Specifications
Flow Rate Range	0.001 to 25 ml/min (up to 50 ml/min during column packing)
Flow Rate Accuracy	± 1.2%
Flow Rate Precision	RSD <0.5%
Pressure Range	0 to 20 MPa (2900 psi)
Pressure Limits	Programmable upper and lower
Viscosity Range	0.35 to 10 cP (5 cP above 12.5 ml.min)
UV Monitor Specifications
Wavelength Range	280 nm (Fixed)
Absorbance Range	-6 to 6 AU
Resolution	0.001 mAU
Wavelength Reproducibility	± 0.01 nm
Linearity	Within ± 5% at 0 to 2 AU
Drift	≤ | 0.2 mAU | AU/h (2 mm cell at 280 nm)
Noise	< 0.1 mAU
Operating Pressure	0 to 2 MPa
Lamp Operating Time	> 10,000 hours
Flow Cell	Optical path length: 2 mm Cell volume: 2 ul Total volume: 30 ul
Conductivity Monitor Specifications
Conductivity Range	0.01 to 999.9 mS/cm
Accuracy	± 0.01 mS/cm or ± 2%, whichever is greater (within 0.3 to 300 mS/cm)
Operating Pressure	0 to 5 MPa
Flow Cell Volume	22 ul
Temperature Monitor Range	0°C to 99°C
Temperature Monitor Accuracy	± 1.5°C within 4°C to 45°C
pH Monitor Specifications
pH Reading Range	0 to 14
Accuracy	± 0.1 pH unit within pH 2 to 12, temperature within ± 3°C from calibration temperature
Operating Pressure	0 to 0.5 MPa
Flow Cell Volume	76 ul
F9-C Fraction Collector Specifications
Number of Fractions	Up to 576
Vessel Types	Deep well plates, 96, 48, or 24 wells
Fraction Volumes	0.1 to 250 ml
Spillage-Free Mode	Automatic, Drop Sync, or Accumulator
Flammable Liquids	No
Delay Volume (UV-Dispenser Head)	205 ul, 86 ul with optional tubing kit (i.d. 0.25 mm)
Dimensions (W x D x H)	15.4 x 23 x 12.6 (39 x 58.5 x 32 cm)
Weight	46.3 lb. (21 kg)

Computer Specifications
Model	Dell Optiplex 7050 Desktop PC
Processor	Intel Core i5-6500 Processor 3.6GHz
RAM	16GB
Hard Drive	250GB Internal SSD
Operating System	Windows 10 Professional, 64-bit

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