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GE AKTA Prime Plus FPLC System
Flow Rate Range
0.1 to 50 mL/min
Maximum Operating Pressure
1 MPa (145 psi)
Single Wavelength Detection
254 and 280 nm Filters Included
- Versatile chromatography system designed for single-step purifications of proteins and antibodies
- Compatible with prepacked columns like HiTrap, HiPrep, and HiLoad for easy setup
- Offers high reproducibility in a compact and user-friendly design with pre-programmed templates
- Flow rate ranges from 0.1 mL/min to 50 mL/min, accommodating various purification scales
- Maximum operating pressure of 1 MPa (145 psi) for robust performance
- Includes single-wavelength detection with a mercury lamp and filters for 254 and 280 nm
- Features two buffer inlets for flexible buffer selection during purification processes
- Designed to work within a relative humidity range of 20% to 95% (non-condensing) and pH stability long term of 2 to 12
The GE AKTA Prime Plus is a flexible chromatography system that performs single-step purifications of tagged and untagged proteins as well as antibodies. For optimal results, use prepacked columns, such as the HiTrap, HiPrep, and HiLoad columns that simply snap onto the side of the unit. The AKTA Prime Plus offers high reproducibility in a compact and easy-to-use design with pre-programmed templates.
The GE AKTA Prime Plus FPLC System is flexible for more general purification tasks, such as ion exchange, affinity chromatography, buffer exchange, HIC, and gel filtration.
PH Electrode Not Included.
The GE AKTA Prime Plus FPLC System includes:
- > Dell Laptop with PrimeView Software
- > Conductivity Flow Cell
- > Built in Fraction Collector
- > UV Lamp & Detector
- > Power Cords and Cables
- > Installation Calibration Report
- > Operator's Guide
- > Full 180 Day Parts and Labor Warranty
GE AKTA Prime Plus FPLC System Specifications
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Flow Rate
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0.1mL/min to 50mL/min
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Maximum Operating Pressure
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1 MPa (145 psi)
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Tubing ID (Flow Path)
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0.75 mm
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Single Wavelength Detection
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Mercury lamp and 254 and 280 nm filters included. Optional filters
for 313, 365, 405, 436, and 546 nm (through manufacturer). Optional zink lamp
with 214 nm filter (through manufacturer).
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Flow Cell Path Length
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2 mm (optional 5 mm through
manufacturer)
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Buffer Selection
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2 inlets
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Relative Humidity
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20% to 95% (non-condensing)
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pH Stability Long Term
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2 to 12
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Viscosity
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Max 10 cP (< 10 ml/min), max 5 cP (>10 ml/min)
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Dimensions (W x H x D)
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400 x 530 x 250 mm
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AKTA Prime Plus Manual
- Inspect all displays, replacing any dim or dead pixels to ensure optimal visual feedback
- Examine internal components of P-900, UV-900/UPC-900, and pH/C-900, replacing any malfunctioning parts to maintain performance
- Verify connections of all components via Uni-Net 1 & 2 interfaces for secure and reliable communication
- Check Uni-Net cables, replacing any faulty ones to ensure uninterrupted data transmission
- Inspect internal components of M-925, INV-907, PV-908, and PV-907 to confirm full operational functionality
- Replace all PEEK tubing with new OEM tubing and inspect all components within the flow path for potential clogs
- Confirm successful software interfacing with the FPLC system for seamless operation
- Replace pump piston seals, wash seals, and seal wash tubing, performing ultrasonic cleaning on each check valve in the pump flow path
- Calibrate pressure and flow rates of both the P-900 pump and P-960/950 pump (if applicable) for precise control
- Replace UV lamp and perform internal inspection tests to confirm complete functionality
- Install a new pH probe and verify the conductivity cell for accurate readings
- Calculate and adjust delay volume in software to reflect system configuration
- Clean or replace the drop sensor, accumulator wash, and height adjuster for consistent collection performance
- Verify fraction collector alignment and calibration with 15mL, 50mL, and microplate receptacles to ensure accuracy
- Run installation tests and thoroughly document all findings for comprehensive record-keeping
Technician Inspection and Recertification Checklist: FPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
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Task
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Completed?
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Remarks?
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Inspect all displays and replace dim/dead pixels
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Completed
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Examine internal components of P-900, UV-900/UPC-900, and pH/C-900
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Completed
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Verify connections via Uni-Net 1 & 2 interfaces
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Completed
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Check and replace any malfunctioning Uni-Net cables
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Completed
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Inspect internal components of M-925, INV-907, PV-908, PV-907
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Completed
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Replace PEEK tubing with OEM tubing and check for clogs
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Completed
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Confirm software interfacing with FPLC system
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Completed
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Replace pump seals and wash tubing; clean check valves
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Completed
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Calibrate pressure and flow rates of P-900 and P-960/950 pumps
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Completed
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Replace UV lamp and confirm full functionality
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Completed
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Install new pH probe and verify conductivity cell
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Completed
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Calculate delay volume and update in software
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Completed
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Clean or replace drop sensor, accumulator wash, and height adjuster
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Completed
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Verify fraction collector alignment with 15mL, 50mL, and microplate receptacles
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Completed
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Run installation tests and document all findings
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Completed
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To View More of Our Recertification Protocol Click Here
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