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Popular Alternative Choice
GE AKTA Purifier 10 FPLC System w/ UPC-900
- Versatile and modular liquid chromatography system designed for the purification of proteins, peptides, and nucleic acids
- Operates at flow rates ranging from 0.001 to 10 ml/min and can handle pressures up to 25 MPa
- Includes a P-900 Pump, UPC-900 Monitor, M-925 Mixer, Frac-920 Fraction Collector, and more
- Hassle-Free 180 Day Full Parts and Labor Warranty
Go to Product Details
Typically Ships Within 3 to 5 Business Days!
GE AKTA FPLC System
The GE AKTA FPLC System is a
biocompatible, high performance liquid chromatography system for the fast and
easy purification of proteins. Providing flexibility and reliability in every
run, the AKTA system provides a unique combination of flow configuration and
methods support for all chromatographic techniques including gel filtration
(size exclusion) chromatography, anion exchange chromatography, cation exchange
chromatography, hydrophobic interaction chromatography, and affinity
chromatography. The compact and flexible design with an expandable rack allows
the use of longer chromatographic columns and automation kits. The AKTA FPLC
fully meets the demands of modern purification media by utilizing AKTAdesign
and UNICORN to provide reliable results and system versatility. This system features flow rates up to 20 ml/min and pressures up to 5 MPa across one working platform for scale-up production.
The P-920 pump provides highly accurate and reproducible flow rates over the whole pressure range. Its chemical resistance makes it possible to use with corrosive liquids, such as organic solvents, as well as with high salt aqueous solutions. The P-920's high flow range makes it suitable for both analytical and preparative chromatography, and it is designed to work with a wide range of columns and gels from the UPC-900 monitor. The monitor provides precise on-line measurements of UV absorption, pH, and conductivity in liquid chromatography. The UPC-900 offers fixed wavelengths of 214 nm, 254 nm, or 280 nm with fast response. Also included is the M-925 mixer for a dynamic, single chamber unit which provides accuracy and reproducibility. The seven-port motorized valve, INV-907, can easily be used to load or wash the sample loop or wash the pump. The Frac-920 provides fraction collection up to 175 fractions in 12 mm tubes, or 95 fractions using 10-18mm tubes, or 40 fractions with 30 mm diameter tubes, depending on which rack is being used.
All of our equipment is fully tested to perform at factory
specifications before being shipped.
The GE AKTA FPLC System includes:
- > IBM Windows 7 workstation loaded with Unicorn 5.31 Software
- > UPC-900 module (U=UV; P=pH; C=Conductivity)
- > P-920 module (with syringe pump)
- > CU 950 Communications Module
- > M-925 Mixer (Outlet Valves)
- > INV-907 (Injection Valve)
- > Conductivity Flow Cell
- > PH electrode
- > Optical Module
- > Frac-920 (Fraction Collector)
- > Gradient Linearity and Step Response Tests
- > All Cables and Power Cords
- > Installation Calibration Report
- > Operator's Guide
- > Full 180 Day Parts and Labor Warranty
GE AKTA FPLC Specifications
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Pump
P-920
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Flow rate range isocratic mode gradient mode
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0.05–20 ml/min in steps of 10 µl/min 0.1–20 ml/min in steps of 10
µl/min
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Pressure range
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0–5 MPa (50 bar, 725 psi)
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Pressure pulsation
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Max. 6% (dP/P) during pump stroke
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pH stability range
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1–13 (1–14 < 1 day exposure)
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Viscosity
< 10 ml/min
> 10 ml/min
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Max. 10cP
Max. 5 cP
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Flow rate reproducibility
flow rate 0.5–10 ml/min
flow rate 10–20 ml/min
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rsd < 0.2%
rsd < 0.5%
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Gradient composition
accuracy between turnings
accuracy during turnings
reproducibility
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± 2% at 0.5–5 ml/min and < 5 MPa
± 2% at 0.5–5 ml/min and
0.5–2.0 MPa
rsd < 0.5% at 0.5–20 ml/min
and < 5 MPa
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Leakage
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< 0.5 µl/min (pump module A and B each)
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Monitor
UPC-900 UV measurement
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Absorbance range
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0.01–5.0 AU (full scale)
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Autozero range
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-0.2–2.0 AU
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Baseline adjust
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Adjustable 0–100% of full scale
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Wavelengths
Hg lamp, fixed
by changing filter
Zn lamp
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254 and 280 nm
313, 365, 405, 436 and 546 nm
214 nm
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UV
Flow cell, 2 mm
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Flow rate
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0–100 ml/min
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Max. pressure
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4.0 MPa (40 bar, 580 psi)
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Max. back-pressure
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0.05 MPa at 100 ml/min
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Liquid temperature range
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+4 to +60 °C
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Optical path length
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2 mm
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Cell volume
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2 µl (30 µl detector volume)
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UV
flow cell, 5 mm
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Flow rate
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0–20 ml/min
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Max. pressure
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4.0 MPa (40 bar, 580 psi)
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Max. back-pressure
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0.02 MPa at 20 ml/min
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Optical path length
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5 mm
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Cell volume
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6 µl (10 µl detector volume)
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Conductivity Measurement
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Flow rate
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0-100 ml/min
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Max. pressure
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5MPa (50 bar, 725 psi)
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Max. back-pressure
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0.01 MPa at 100 ml/min
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- Inspect all displays, replacing any dim or dead pixels to ensure optimal visual feedback
- Examine internal components of P-900, UV-900/UPC-900, and pH/C-900, replacing any malfunctioning parts to maintain performance
- Verify connections of all components via Uni-Net 1 & 2 interfaces for secure and reliable communication
- Check Uni-Net cables, replacing any faulty ones to ensure uninterrupted data transmission
- Inspect internal components of M-925, INV-907, PV-908, and PV-907 to confirm full operational functionality
- Replace all PEEK tubing with new OEM tubing and inspect all components within the flow path for potential clogs
- Confirm successful software interfacing with the FPLC system for seamless operation
- Replace pump piston seals, wash seals, and seal wash tubing, performing ultrasonic cleaning on each check valve in the pump flow path
- Calibrate pressure and flow rates of both the P-900 pump and P-960/950 pump (if applicable) for precise control
- Replace UV lamp and perform internal inspection tests to confirm complete functionality
- Install a new pH probe and verify the conductivity cell for accurate readings
- Calculate and adjust delay volume in software to reflect system configuration
- Clean or replace the drop sensor, accumulator wash, and height adjuster for consistent collection performance
- Verify fraction collector alignment and calibration with 15mL, 50mL, and microplate receptacles to ensure accuracy
- Run installation tests and thoroughly document all findings for comprehensive record-keeping
Technician Inspection and Recertification Checklist: FPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
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Task
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Completed?
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Remarks?
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Inspect all displays and replace dim/dead pixels
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Completed
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Examine internal components of P-900, UV-900/UPC-900, and pH/C-900
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Completed
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Verify connections via Uni-Net 1 & 2 interfaces
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Completed
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Check and replace any malfunctioning Uni-Net cables
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Completed
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Inspect internal components of M-925, INV-907, PV-908, PV-907
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Completed
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Replace PEEK tubing with OEM tubing and check for clogs
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Completed
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Confirm software interfacing with FPLC system
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Completed
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Replace pump seals and wash tubing; clean check valves
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Completed
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Calibrate pressure and flow rates of P-900 and P-960/950 pumps
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Completed
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Replace UV lamp and confirm full functionality
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Completed
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Install new pH probe and verify conductivity cell
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Completed
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Calculate delay volume and update in software
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Completed
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Clean or replace drop sensor, accumulator wash, and height adjuster
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Completed
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Verify fraction collector alignment with 15mL, 50mL, and microplate receptacles
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Completed
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Run installation tests and document all findings
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Completed
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To View More of Our Recertification Protocol Click Here
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