 |
 |
 |
|
GE AKTA FPLC Explorer 100 System
Wavelength Range
190–700 nm in steps of 1 nm
Wavelength Accuracy
±2 nm
Max. Flow Rate
100 ml/min
- Supports size exclusion, ion exchange, and affinity chromatography.
- Six interchangeable mixing chambers, accommodating flow rates from 0.001 to 100 ml/min, and can handle pressures up to 35 MPa.
- Ensures smooth operation and integration with industry-standard Unicorn 5.31 software.
- Allows for fast pH optimization, enhancing the efficiency of purification processes.
- Ideal for protein purification in research and biotechnology, including proteomics, drug discovery, biochemistry, and molecular biology.
The GE ÄKTA FPLC Explorer 100 System is designed for a wide range of applications in research and biotechnology, catering to the diverse needs of scientists working on protein purification. Its versatility allows for use in size exclusion, ion exchange, and affinity chromatography, making it ideal for studying protein interactions, characterizing protein structure, and isolating proteins for further downstream analysis. This system is particularly useful in fields such as proteomics, drug discovery, biochemistry, and molecular biology, offering reliable performance and accurate results in the purification of recombinant proteins, antibodies, and protein complexes. Choose the ÄKTA Explorer 100 System for a comprehensive and adaptable solution for your protein purification needs.
The GE ÄKTA FPLC Explorer 100 System is a versatile and powerful solution for protein purification. This system features a UV-900 Detector with a wavelength range of 190-700 nm, offering simultaneous detection at three wavelengths with 4 nm bandwidth, and a flow cell capable of handling up to 100 ml/min with a maximum pressure of 2 MPa. The P-900 Pump ensures accurate and reproducible flow rates ranging from 0.01 to 100 ml/min in both isocratic and gradient modes. The M-925 Mixer, with six interchangeable mixing chambers, accommodates flow rates from 0.001 to 100 ml/min and can handle pressures up to 35 MPa, depending on the chamber. The Frac-950 Fraction Collector offers a wide range of fraction sizes and flow rates, with a pH stability range of 1 to 13. Trust the ÄKTA Explorer 100 System for efficient and reliable protein purification in your research and biotechnology applications.
This GE AKTA Explorer 100 FPLC System is in excellent condition and has passed all tests for full functionality. These tests included: linking the unit to the Unicorn 5.31 operating system, testing flow rate accuracy of P-900 Pump, testing operation of pressure sensor in P-900 and P-950, leak-testing P-900 and P-950, testing UV-900 Monitor with Nal test solution, testing pH/C-900 for functional communication with pH probe and Conductivity Cell, testing functionality of the 925 Sensor, testing motor valves, and testing the Frac-950 for functionality through manual commands. Configured setup for detection is UV Absorbance, pH, and Conductivity. Fraction collection is through the Frac-950.
The GE AKTA FPLC Explorer 100 System includes:
- > Box-900
- > P-900 Pump w/Switch Valves
- > UV-900 Detector
- > UV Flow Cell
- > New UV Lamp
- > pH/C-900 Monitor
- > P-960 Sample Pump
- > M-925 Mixer
- > 2mL Mixing Chamber M-925
- > Conductivity Cell
- > pH Probe and Holder
- > CU-950 Control Box
- > Injection V1 INV-907
- > Column V2 PV-908
- > Column V3 PV-908
- > Outlet V4 PV-908
- > Sample V5 Valve PV-908
- > Buffer Valve V6 IV-908
- > Flow Direction Valve V7 INV-907
- > FR-902 Flow Restrictor
- > Inline Filter and Tubing
- > Frac-950 Fraction Collector & Rack
- > Dell Datastation running Windows 7 Pro with Unicorn version 5.31 software
- > Flat Screen Monitor
- > Installation Calibration Report
- > Communication and Power Cables
- > Full 180 Day Parts and Labor Warranty
GE AKTA FPLC Explorer 100 System Specifications
|
UV-900 Detector Specifications
|
|
Wavelength
range
|
190–700 nm in
steps of 1 nm,
3 wavelengths
simultaneously
|
|
Bandwidth
|
4 nm
|
|
Wavelength
accuracy
|
±2 nm
|
|
Wavelength
reproducibility
|
< 500 ms
(one cycle from 214 nm to
254 nm and
back to 214 nm)
|
|
Linearity
|
< 2%
deviation up to 2 AU at 260 nm with
Uracil at pH
2
|
|
Static noise
|
short term:
< 6x10-5 AU at 230 nm
long term:
< 6x10-5 AU at 230 nm
|
|
Drift
|
< 2x10-4
AU/h at 254 nm
|
|
Flow Cell
|
|
Optical path
length 2 mm cell
|
2 mm, 2 µl
cell volume
|
|
Optical path
length 10 mm cell
|
10 mm, 8 µl
cell volume
|
|
Max. flow
rate
|
100 ml/min
|
|
Max. pressure
|
2 MPa (290
psi)
|
|
Temperature
range
|
+4 to +40 °C
(liquid)
|
|
Wetted
material
|
PTFE
(polytetrafluoroethylene)
PEEK
(polyetheretherketone)
titanium
(palladium alloy),
quartz
(synthetic fused silica)
and titanium
|
|
P-900 Pump Specifications
|
|
Flow Rate
Range
|
Isocratic
mode: 0.01 to 100 ml/min in steps of 10 ul/min
Gradient
mode: 0.01 to 100 ml/min in steps of 10 ul/min
Double mode:
0.01 to 200 ml/min in steps of 10 ul/min
|
|
Pressure
Range
|
0 to 10 MPa
(100 bar, 1450 psi)
|
|
pH Stability
Range
|
1 to 13 (1 to
14 with <1 day exposure)
|
|
Viscosity
|
Max 5 cP
|
|
Flow Rate
Accuracy
|
± 2% or 20
ul/min, whichever is greater
|
|
Flow Rate
Reproducibility
|
Flow
rate ≥ 0.5 ml/min: rsd < 0.5%
|
|
Gradient
Composition
|
Accuracy:
< ±1% at 0.05 to 10 ml/min
Reproducibility:
rsd < 0.25% at 0.5 to 100 ml/min
|
|
Pressure
Sensor
|
Range: 0 to
27.5 MPa
Offset error:
< 0.05 MPa
Scale error:
< ±2%
|
|
Total Volume
Between Inlet and Outlet
|
|
|
M-925 Mixer Specifications
|
|
Max Flow Rate
|
0.001 to 100
ml/min
|
|
Max Pressure
|
12ml chamber:
10 MPa at 0.1 to 100 ml/min (100 bar, 1450 psi)
0.6, 2, and
5ml chambers: 25 MPa at 0.001 to 100 ml/min (250 bar, 3625 psi)
90 ul and 0.2
ml chambers: 35 MPa at 0.001 to 5 ml/min (250 bar, 5075 psi)
|
|
pH Stability
Range
|
1 to 13 (1 to
14 with <1 day exposure)
|
|
Viscosity
|
Max 5 cP
|
|
Internal
Volume
|
6
interchangeable mixing chambers: 90 ul, 0.2, 0.6, 2, 5, and 12 ml
|
|
Mixing
Principle
|
1 static
chamber and 1 dynamic chamber
|
|
Inlet and
Outlet Tubing
|
UNF 10 to 32
thread profile
|
|
Frac-950 Fraction Collector Specifications
|
|
Max Flow Rate
|
100 ml/min
|
|
pH Stability
Range
|
1 to 13 (1 to
14 with <1 day exposure)
|
|
Fraction Size
|
0.1 to
99999.99 ml (volume mode)
0.1 to
99999.99 min (time mode)
|
|
Standard Mode
|
u-liter
plates, DropSync: 0 to 1 ml/min
12 mm rack,
DropSync: 0 to 1.5 ml/min
18 and 30 mm
racks, DropSync: 0 to 2 ml/min
18 and 30 mm racks, Accumulator: 15 to 100ml/min
|
|
Prep Mode
|
30 mm rack, Accumulator: 15 to 100 ml/min
Funnel-to-flask, Accumulator: 15 to 100 ml/min
250ml bottles, Accumulator: 15 to 100 ml/min
|
|
Environmental Conditions
|
+4° to +40°C
20 to 95% relative humidity
84 to 106 kPa (840 to 1060 mbar) atmospheric pressure
|
- Inspect all displays, replacing any dim or dead pixels to ensure optimal visual feedback
- Examine internal components of P-900, UV-900/UPC-900, and pH/C-900, replacing any malfunctioning parts to maintain performance
- Verify connections of all components via Uni-Net 1 & 2 interfaces for secure and reliable communication
- Check Uni-Net cables, replacing any faulty ones to ensure uninterrupted data transmission
- Inspect internal components of M-925, INV-907, PV-908, and PV-907 to confirm full operational functionality
- Replace all PEEK tubing with new OEM tubing and inspect all components within the flow path for potential clogs
- Confirm successful software interfacing with the FPLC system for seamless operation
- Replace pump piston seals, wash seals, and seal wash tubing, performing ultrasonic cleaning on each check valve in the pump flow path
- Calibrate pressure and flow rates of both the P-900 pump and P-960/950 pump (if applicable) for precise control
- Replace UV lamp and perform internal inspection tests to confirm complete functionality
- Install a new pH probe and verify the conductivity cell for accurate readings
- Calculate and adjust delay volume in software to reflect system configuration
- Clean or replace the drop sensor, accumulator wash, and height adjuster for consistent collection performance
- Verify fraction collector alignment and calibration with 15mL, 50mL, and microplate receptacles to ensure accuracy
- Run installation tests and thoroughly document all findings for comprehensive record-keeping
Technician Inspection and Recertification Checklist: FPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
|
Task
|
Completed?
|
Remarks?
|
|
Inspect all displays and replace dim/dead pixels
|
Completed
|
|
|
Examine internal components of P-900, UV-900/UPC-900, and pH/C-900
|
Completed
|
|
|
Verify connections via Uni-Net 1 & 2 interfaces
|
Completed
|
|
|
Check and replace any malfunctioning Uni-Net cables
|
Completed
|
|
|
Inspect internal components of M-925, INV-907, PV-908, PV-907
|
Completed
|
|
|
Replace PEEK tubing with OEM tubing and check for clogs
|
Completed
|
|
|
Confirm software interfacing with FPLC system
|
Completed
|
|
|
Replace pump seals and wash tubing; clean check valves
|
Completed
|
|
|
Calibrate pressure and flow rates of P-900 and P-960/950 pumps
|
Completed
|
|
|
Replace UV lamp and confirm full functionality
|
Completed
|
|
|
Install new pH probe and verify conductivity cell
|
Completed
|
|
|
Calculate delay volume and update in software
|
Completed
|
|
|
Clean or replace drop sensor, accumulator wash, and height adjuster
|
Completed
|
|
|
Verify fraction collector alignment with 15mL, 50mL, and microplate receptacles
|
Completed
|
|
|
Run installation tests and document all findings
|
Completed
|
|
To View More of Our Recertification Protocol Click Here
|
|
|
 |
|
 |
|
