Fluorescence Microplate Readers
Absorbance Photometric Performance
Wavelength Selection
Monochromator, tunable in 1 nm increments
Wavelength Range
200 to 1000 nm
Wavelength Bandwidth
≤ 4.0 nm full width half maximum
Wavelength Accuracy
± 2.0 nm across wavelength range
Wavelength Repeatability
± 0.2 nm
Photometric Range
0.000 to 4.000 OD
Photometric Resolution
0.001 OD
Photometric Accuracy (Microplate)
< ±0.006 OD ±1%, 0 to 2 OD
Photometric Accuracy (Cuvette)
< ±0.005 OD ±1%, 0 to 2 OD
Photometric Precision
< ±0.003 OD ±1%, 0 to 2 OD
Baseline Flatness
< 0.001 OD
Stray Light
< 0.05% @ 230 nm
Fluorescence Intensity Performance
Dual Monochromators
1 nm increment selection
EX Wavelength Range
250 to 850 nm
EM Wavelength Range
Wavelength Bandwidth (EX, EM)
9 nm
Top-Read Optimized Sensitivity
≤ 1 pM (fluorescein) in 96 wells≤ 1.5 pM (fluorescein) in 384 wells
Top-Read Guaranteed Sensitivity
< 5 pM (fluorescein) in 96 wells or cuvette< 20 pM in 384 wells
Luminescence Photometric Performance
Sensitivity
< 2 fg/well for firefly luciferase in 96- and 384-well microplates (top read)
Crosstalk
< 0.5% in 96- and 384- well microplates
Dynamic Range
Optimized Sensitivity
≤ 43 pM ATP in 96 wells
Guaranteed Sensitivity
≤ 75 pM ATP in 96 wells
General Photometric Performance
Plate Formats
6, 12, 24, 48, 96, 384 wells
Light Source
Xenon flash lamp (1 joule/flash)
Detectors
2 Photomultiplier tubes (PMT)
Read Time
96 wells: Abs 18 sec., Fl 17 sec., Lumi 120 sec.
384 wells: Abs 49 sec., Fl 48 sec., Lumi 420 sec.
Shaker Time
0 to 999 seconds
Temperature Control
Ambient +2°C to 60°C
Temperature Uniformity
< 1°C at 37°C set point
Temperature Accuracy
±1°C at 37°C set point
General Specifications
Dimensions (H x W x D)
21.8 x 51.2 x 39 cm (8.6 x 22.5 x 15.4 in)
Weight
16.4 kg (36 lbs)
≤ 4.0 nm
± 2.0 nm
0 to 4.0 OD
Fluorescence Photometric Performance
9 nm, 9 nm
5 pM (fluorescein) in 96 wells
15 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
360 to 850 nm
Data Collection
50 to 1450 µsec., 200 µsec. increments
7 pM Eu-chelate
Luminescence (secondary mode)
50 fM alkaline phosphatase
Detector
Photomultiplier tube (PMT)
96 wells: Abs 18 sec., Fl 15 sec.
384 wells: Abs 49 sec., Fl 45 sec.
Ambient +4C to 45C
< 1C at 37C set point
± 1C at 37C set point
UV/Vis Photometric Performance:
Monochromator, tunable in 1.0 nm increments
± 4.0 nm
< ± 0.006 OD ± 1.0%, 0-2 OD
< ± 0.003 OD ± 1.0%, 0-2 OD
Fluorescence Photometric:
1 nm increments
EX 250 to 850 nm
EM 360 to 850 nm
Bandwidth (EX, EM)
9, 9 nm
Detection Limit
3.0 fmol/well FITC 200µl in 96 wells
Electrical Requirements
100-240 VAC, 50/60 Hz
Performance
Wavelength Range Excitation
Wavelength Range Emission
Wavelength Selections
Monochromator, tunable 1.0 nm increments
Wavelength
< ± 2.0 nm
Top Read Sensitivity
3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity
8.0 fmol/well FITC 200 µl in 96 wells
Xenon flash lamp
Microplate Read Time
Normal Mode
96 wells in 15 seconds
384 wells in 45 seconds
6 orders of magnitude
Temperature Range
Ambient + 4° up to 45°C
± 1° at 37°C
±1C at 37C set point
1nm increments
250-850nm
Bandwidth (Excitation & Emission)
Time Resolved Fluorescence
50-1450 µ/sec, 200µ/sec increments
0.5/ well
Luminescence Performance
360-850nm
10 amol/well Alkaline Phosphate 200 µl/well
Microplate Formats
6-, 12-, 24-, 48-, 96- and 384
Photomultiplier (R-3896)
96 < 15 sec, 384 < 45 sec
Ambient +4°C - 45°C