Electrical Requirements
100-240 VAC, 50/60 Hz
Performance
Wavelength Range Excitation
250 to 850 nm
Wavelength Range Emission
Wavelength Selections
Monochromator, tunable 1.0 nm increments
Wavelength Bandwidth
9 nm
Wavelength
< ± 2.0 nm
Top Read Sensitivity
3.0 fmol/well FITC 200 µl in 96 wells
Bottom Read Sensitivity
8.0 fmol/well FITC 200 µl in 96 wells
Light Source
Xenon flash lamp
Microplate Read Time
Normal Mode
96 wells in 15 seconds
384 wells in 45 seconds
Dynamic Range
6 orders of magnitude
Temperature Range
Ambient + 4° up to 45°C
Temperature Uniformity
± 1° at 37°C
Absorbance Photometric Performance
Wavelength Selection
Monochromator, tunable in 1 nm increments
Wavelength Range
200 to 1000 nm
± 4.0 nm
Wavelength Accuracy
± 2.0 nm
Wavelength Repeatability
± 0.2 nm
Photometric Range
0 to 4.0 OD
Photometric Resolution
0.001 OD
Photometric Accuracy (Microplate)
< ±0.006 OD ±1%, 0 to 2 OD
Photometric Accuracy (Cuvette)
< ±0.005 OD ±1%, 0 to 2 OD
Photometric Precision
< ±0.003 OD ±1%, 0 to 2 OD
Baseline Flatness
< 0.001 OD
Stray Light
< 0.05% @ 230 nm
Fluorescence Photometric Performance
Dual Monochromators
1 nm increment selection
EX Wavelength Range
EM Wavelength Range
Wavelength Bandwidth (EX, EM)
Top-Read Optimized Sensitivity
5 pM (fluorescein) in 96 wells
Top-Read Guaranteed Sensitivity
15 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
360 to 850 nm
Data Collection
50 to 1450 µsec., 200 µsec. increments
Guaranteed Sensitivity
7 pM Eu-chelate
Luminescence (secondary mode)
50 fM alkaline phosphatase
General Photometric Performance
Plate Formats
6, 12, 24, 48, 96, 384 wells
Xenon flash lamp (1 joule/flash)
Detector
Photomultiplier tube (PMT)
Read Time
96 wells: Abs 18 sec., Fl 15 sec.
384 wells: Abs 49 sec., Fl 45 sec.
Shaker Time
0 to 999 seconds
Temperature Control
Ambient +4C to 45C
< 1C at 37C set point
Temperature Accuracy
±1C at 37C set point
≤ 4.0 nm
9 nm, 9 nm
± 1C at 37C set point
UV/Vis Photometric Performance:
Monochromator, tunable in 1.0 nm increments
< ± 0.006 OD ± 1.0%, 0-2 OD
< ± 0.003 OD ± 1.0%, 0-2 OD
Fluorescence Photometric:
1 nm increments
EX 250 to 850 nm
EM 360 to 850 nm
Bandwidth (EX, EM)
9, 9 nm
Detection Limit
3.0 fmol/well FITC 200µl in 96 wells
≤ 4.0 nm full width half maximum
± 2.0 nm across wavelength range
0.000 to 4.000 OD
Fluorescence Intensity Performance
≤ 1 pM (fluorescein) in 96 wells≤ 1.5 pM (fluorescein) in 384 wells
< 5 pM (fluorescein) in 96 wells or cuvette< 20 pM in 384 wells
Luminescence Photometric Performance
Sensitivity
< 2 fg/well for firefly luciferase in 96- and 384-well microplates (top read)
Crosstalk
< 0.5% in 96- and 384- well microplates
Optimized Sensitivity
≤ 43 pM ATP in 96 wells
≤ 75 pM ATP in 96 wells
Detectors
2 Photomultiplier tubes (PMT)
96 wells: Abs 18 sec., Fl 17 sec., Lumi 120 sec.
384 wells: Abs 49 sec., Fl 48 sec., Lumi 420 sec.
Ambient +2°C to 60°C
< 1°C at 37°C set point
±1°C at 37°C set point
General Specifications
Dimensions (H x W x D)
21.8 x 51.2 x 39 cm (8.6 x 22.5 x 15.4 in)
Weight
16.4 kg (36 lbs)
Absorbance Specifications
Photodiode
Monochromator
200 to 999 nm, in 1 nm increments
Monochromator Bandwidth
2.4 nm
Monochromator Wavelength Accuracy
± 2 nm
Monochromator Wavelength Repeatability
0.0001 OD
Pathlength Correction
Yes
Photometric Linearity
< 1% from 0 to 3.0 OD
Photometric Repeatability
< 0.5% at 2.0 OD
Fluorescence Intensity Specifications
Tungsten halogen
PMT
Filter-based
300 to 700 nm
> 6 decades
Sensitivity (Top and Bottom)
Fluorescein 5 pM (1 fmol/well, 96-well plate)
Luminescence Specifications
< 6 decades
30 amol ATP (flash)
Detection System
Low noise PMT
Alpha Detection Specifications
300 amol of biotinylated LCK-P peptide
Read Speed
2 minutes (96-well plate)
Microplate Formats
Read Methods
End point, kinetic, spectral scanning, well-area scanning
Plate Shaking
Linear and orbital
4-Zone incubation to 50°C, ± 0.2°C at 37°C
12 x 15 x 15 in. (30.5 x 38.1 x 38.1 cm)
27 lb. (12.3 kg.)
100-240V, 50/60Hz
