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Eppendorf 5420 Centrifuge
Maxiumum Capacity
24 x 1.5/2.0ml
- Designed specifically for molecular biology applications, including DNA/RNA purification and gel extraction
- Compact footprint ideal for saving space on lab benches
- Soft-touch one-finger lid closure for ergonomic operation
- Automatic lid opening at end of run to prevent sample warming and facilitate easy access
- Equipped with high-quality anodized aluminum rotors and rotor lids for durability
- Features a one-push short-spin key for quick and convenient centrifugation
- Timer ranges from 10 seconds to 9:59 hours with a continuous run option
- Easy-to-clean keypad control panel enhances user convenience
The Eppendorf 5420 Centrifuge is specifically engineered to enhance your molecular biology applications, particularly in spin column-based nucleic acid purification and gel extraction. This centrifuge combines high-speed capabilities with a compact design, achieving speeds up to 15,060 RPM and a maximum RCF of 21,300 x g. It accommodates up to 24 Eppendorf tubes ranging from 1.5 to 2.0 mL within its aerosol-tight rotor, ensuring efficient and safe sample handling. The unit's small footprint is ideal for maximizing lab bench space, while its user-friendly features include an easy-to-clean keypad and ergonomic lid locking mechanism. The soft-touch, one-finger lid closure facilitates convenient operation, and the automatic lid opening feature at the end of each run prevents sample warming and provides quick access to tubes. Additionally, the centrifuge is equipped with high-quality anodized aluminum rotors and lids, enhancing its durability. The inclusion of a one-push short-spin key offers rapid centrifugation without the need to continuously press the key, making the Eppendorf 5420 Centrifuge a reliable and essential tool for any laboratory conducting DNA/RNA purification, DNA extraction from agarose gels, and PCR reaction setup.
The included FA-24x2 Fixed Angle Rotor reaches a maximum speed of 15,060 RPM.
The Eppendorf 5420 Centrifuge includes:
- > FA-24x2 Fixed Angle Rotor with Aerosol Lid
- > NIST Centrifuge Calibration and Preventative Maintenance Report
- > Operator's Manual
- > Power Cord
- > Full 180 Day Parts and Labor Warranty
Eppendorf 5420 Centrifuge Specifications
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Maximum Speed
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15,060 RPM
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Minimum Speed
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100 RPM
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Maximum RCF
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21,300 x g
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Maximum Capacity
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24 x 1.5/2.0ml
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Temperature Range
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N/A
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Timer
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10 seconds to 9:59 hours, with continuous run
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Acceleration Time
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15 seconds
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Deceleration Time
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15 seconds
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Number of Compatible Rotors
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2
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Noise Level
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< 56 dB(A)
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Maximum Kinetic Energy
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4.09 kJ
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Dimensions (W x D x H)
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9.4 x 13.8 x 8.7 in. (24 x 35 x 22 cm)
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Weight
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28.7 lb. (13 kg)
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Electrical Requirements
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120V, 50/60Hz, 4A, 270W
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Refrigeration
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No Refrigeration
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- Confirm the power supply output voltages match specified requirements for stable distribution to all internal components
- Measure and verify voltages at PCB test points to ensure accurate power delivery and circuit integrity
- Confirm rotor compatibility with the centrifuge model and check that rotor detection systems recognize the rotor type
- Inspect the exterior for any cosmetic or structural damage that could affect performance or safety
- Check the door mechanism alignment to ensure secure and smooth opening and closing
- Inspect and replace the gas strut with an OEM part to maintain proper door support and safety
- Examine the rotor chamber bowl for dents or damage and replace as needed to ensure safe operation
- Replace and confirm full functionality of all lid lock switches to maintain secure closure
- Inspect and lubricate the door lock mechanism, applying grease to the lid lock rail for smooth operation
- Test compressor functionality (if applicable) to ensure proper operation and consistent temperature control
- Conduct an 8-12 hour cycle test to validate consistent performance over prolonged use
- Confirm rotor motor stability, check for unusual noise, and assess mechanical integrity during operation
- Inspect the condensation drain and clear any clogs to prevent moisture buildup in the rotor chamber
- Verify RPM accuracy using an NIST-traceable tachometer and perform calibration as necessary
- Check the centrifuge error log to ensure zero recorded issues, confirming system reliability
Technician Inspection and Recertification Checklist: Centrifuges
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
| Task |
Completed? |
Remarks? |
| Confirm power supply output voltages match specified requirements |
Completed |
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| Measure and verify voltages at PCB test points for accuracy |
Completed |
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| Confirm rotor compatibility and detection for proper identification |
Completed |
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| Inspect exterior for cosmetic or structural damage |
Completed |
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| Check door mechanism alignment for secure opening and closing |
Completed |
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| Inspect and replace gas strut with OEM part as needed |
Completed |
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| Examine rotor chamber for dents or damage and replace if necessary |
Completed |
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| Replace and confirm functionality of all lid lock switches |
Completed |
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| Lubricate door lock mechanism and lid lock rail for smooth operation |
Completed |
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| Test compressor (if applicable) for proper operation and cooling |
Completed |
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| Run 8-12 hour cycle test to ensure consistent performance |
Completed |
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| Check rotor motor for stability, noise, and integrity |
Completed |
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| Inspect and clear condensation drain to prevent buildup |
Completed |
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| Verify RPM accuracy with NIST traceable tachometer and calibrate |
Completed |
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| Check centrifuge error log for zero recorded issues |
Completed |
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To View More of Our Recertification Protocol Click Here
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