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Eppendorf 5333 MasterCycler Thermal Cycler
The Eppendorf 5333 MasterCycler Thermal Cycler is ideal for
quantitative PCR applications, meeting all of the latest requirements with its
outstanding speed and flexibility. The MasterCycler offers extremely fast
temperature ramping, short detection times, and intuitive assay programming,
which saves time to allow multiple accurate and reliable tests to be run per
day.
The Eppendorf 5333 temperature control is carried out with Peltier elements, which enable extremely rapid changes in temperature of up to 3 °C per second. This allows the temperature of the samples to be controlled extremely rapidly between 4 °C and 99 °C.
The block can hold both 0.2ml (capacity of 96) and
0.5ml (capacity of 77) tubes or a microtitre plate (capacity of one), with no
need to change blocks. The Eppendorf 5333 has a small footprint, which makes it
excellent for labs with little space.
All of our equipment is fully tested to perform at factory
specifications before being shipped.
The Eppendorf 5333 MasterCycler Thermal Cycler includes:
- > 96 Well Block
- > Calibration and Preventative Maintenance Report
- > Power Cord
- > Full 180 Day Parts and Labor Warranty
Eppendorf 5333 MasterCycler Thermal Cycler Specifications
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Sample Capacity
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96 x 0.2ml PCR tubes or 77 x 0.5ml PCR tubes or one 8 x 12
microtiter plate
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Temperature Range
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4°C - 99°C
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Temperature Distribution
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95°C ± 1.0°C
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Temperature Accuracy
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± 0.2°C
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Pre-set Programs
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100 built-in and approx 10 slots available for personal
card
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Maximum Cycles
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99
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Eppendorf MasterCycler Thermal Cycler Manual
 Click Here to View all the Technical Specs for the MasterCycler
- Validate that the power supply and PCB consistently deliver accurate, stable voltages across all circuits and connections, confirming operational reliability
- Thoroughly inspect the display for any dim, flickering, or dead pixels, replacing compromised segments to ensure clear visibility and precise data display
- Verify complete functionality and responsiveness of all buttons and knobs, confirming accurate and reliable input across all controls
- Ensure the machine completes all internal diagnostic checks with zero errors, indicating system readiness and functional integrity
- Confirm the machine accurately identifies attached blocks (96-well, dual 48, etc.), with seamless recognition across different configurations
- Conduct a detailed inspection of internal wiring and all electrical connections, checking for any signs of breaks, disconnections, or tears; replace or repair any underperforming components
- Examine the connector between the block and base for bent or broken pins, replacing as needed to maintain secure, reliable connections
- Calibrate well temperatures with a NIST-traceable thermometer, verifying accuracy and uniformity across all wells to meet specification standards
- Perform a comprehensive cosmetic inspection, ensuring all exterior surfaces and components are free of damage, wear, or defects, maintaining aesthetic and professional quality
Technician Inspection and Recertification Checklist: Thermal Cycler
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
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Task
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Completed?
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Remarks?
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Validate stable, accurate voltage outputs from power supply and PCB across circuits
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Completed
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Inspect display for dim, flickering, or dead pixels; replace as needed for clarity
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Completed
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Verify functionality and responsiveness of all buttons and knobs
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Completed
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Confirm machine completes internal diagnostics with zero errors
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Completed
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Ensure accurate block identification across configurations (96-well, dual 48, etc.)
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Completed
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Inspect internal wiring and connections for integrity; repair or replace as needed
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Completed
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Check connector between block and base for bent or broken pins; replace as necessary
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Completed
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Calibrate well temperatures with a NIST-traceable thermometer for accuracy
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Completed
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Conduct cosmetic inspection to ensure quality and professionalism of all surfaces
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Completed
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To View More of Our Recertification Protocol Click Here
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