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Biotek Synergy HT Microplate Reader
Detector Modes
Absorbance, Fluorescence, and Luminescence
Absorbance Accuracy
2.000 to 2.500 OD ± 3.0% ± 0.010 OD Normal and Rapid modes, 96- and 384-well plates
Wavelength Range
Absorbance: 200 to 999 nm
Fluorescence: 300 to 650 nm (Excitation) / 300 to 700 nm (Emission)
Luminescence: 300 to 700 nm
- Single-channel microplate reader for absorbance, fluorescence, and luminescence.
- Computer-controlled using BioTek's Gen5 software for all operations and data analysis.
- Robot accessible and compatible with BioTek's BioStack Microplate Stacker.
- Utilizes tungsten quartz halogen lamp and interference filters for fluorescence measurements.
- Luminescence measured through a low-noise PMT detector with an empty filter position.
- Absorbance measurements made using a xenon flash lamp and monochromator.
- 4-Zone temperature control (4°C to 50°C) for assay flexibility.
- Supports 6-, 12-, 24-, 48-, 96-, and 384-well microplates with standard 128 x 86 mm geometry. Includes internal plate shaking for reagent mixing.
The Biotek Synergy HT is a single-channel absorbance, fluorescence, and luminescence microplate reader. It is computer-controlled using BioTek’s Gen5 or KC4 PC software for all operations including data reduction and analysis. The Synergy HT is robot accessible and compatible with BioTek’s BioStack Microplate Stacker. When making fluorescence determinations, the Synergy HT uses a tungsten quartz halogen lamp with interference filters for wavelength specificity in conjunction with a photomultiplier (PMT) tube detector. Luminescence is measured by the low-noise PMT detector through an empty filter position in the Emission filter wheel. Absorbance measurements are made by switching to a xenon flash lamp and a monochromator for wavelength selection. The Synergy HT has a 4-Zone temperature control from 4°C over ambient to 50°C and supports the reading of 6-, 12-, 24-, 48-, 96-, and 384-well microplates with standard 128 x 86 mm geometry. Internal plate shaking is supported to ensure that reagents are properly mixed prior to reading.
The Biotek Synergy HT Microplate Reader includes:
- > Windows 11 Professional 64-Bit Data Station with Pre-Loaded Gen5 PC Software
- > 460 nm, 528 nm, 590 nm, and 645 nm Emission Filters
- > 360 nm, 485 nm, 530 nm, and 590 nm Excitation Filters
- > ABS, FLD, and LUM Calibration Report
- > Operator's Manual
- > Interface Cable
- > Full 180 Day Part and Labor Warranty
Biotek Synergy HT Multimode Microplate Reader Specifications
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Wavelength Range
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Absorbance: 200 to 999 nm
Fluorescence: 300 to 650 nm (Excitation) / 300 to 700 nm (Emission)
Luminescence: 300 to 700 nm
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Wavelength Bandpass
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2.4 nm
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Wavelength Selection
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Absorbance: Monochromator, ± 1 nm
Fluorescence: Filter Based
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Microplate Capacity
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Standard 6-, 12-, 24-, 48-, 96-, 384-well microplates
Max Plate Height:
Luminescence/Fluorescence modes: plates up to 1.25” (31.75 mm) high
Absorbance mode: plates up to 0.8” (20.30 mm) high
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Absorbance Measurement Range
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0.000 to 4.000 OD
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Absorbance Accuracy
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2.000 to 2.500 OD ± 3.0% ± 0.010 OD Normal and Rapid modes, 96- and 384-well plates
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Absorbance Linearity
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2.000 to 2.500 OD ± 3.0% Normal and Rapid modes, 96- and 384-well plates
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Absorbance Repeatability
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0.000 to 2.000 OD ± 1.0% ± 0.005 OD Normal and Rapid modes, 96- and 384-well plates
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Fluorescence Read Timing
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Excitation Filter: 485/20 nm
Emission Filter: 528/20 nm
96-well read: 89 sec
384-well read: 275 sec
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Fluorescence Sensitivity
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5mm probe: 10 pg/ml solution of Sodium Fluorescein in PBS
3mm probe: 20 pg/ml solution of Sodium Fluorescein in PBS
1.5mm probe: 40 pg/ml solution of Sodium Fluorescein in PBS
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Time Resolved Fluorescence
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Delay 0 or 20 µs to 16000 µs
Integration interval 20 to 16000 µs
Times adjustable in 10 µs increments
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Read Modes
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Normal, Rapid and Sweep
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Top/Bottom Read Capability
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Top & Bottom Fluorescence
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Light Source
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Absorbance: Xe flash light source
Fluorescence: Tungsten quartz halogen
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Incubation
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Temperature control range from 4º over ambient to 50ºC
Temperature variation ± 0.50°C across the plate @ 37°C (250 µL per well with the plate sealed)
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Plate Shaking
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Low, Medium, High and Variable shaking speeds. Shake time is programmable by the user
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Environment
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Operational temperature 18º to 40°C
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Humidity
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10% to 85% relative humidity (non-condensing)
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Power Consumption
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100 VA max, 130 VA max with injectors
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Dimensions (D x W x H)
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16 x 15 x 10 in (40.6 cm x 38 cm x 25.4 cm)
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Weight
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38 lb. (17 kg)
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Computer Specifications
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Processor
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Intel Core I5-8350U CPU
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RAM
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16.0 GB
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Hard Drive
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512GB Internal SSD
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Operating System
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Windows 11 Professional, 64-bit
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Frequently Purchased With
- Confirm the power supply and PCB output correct, stable voltages across all required connections
- Inspect the display for any dim or dead pixels; replace affected components to maintain full visual clarity and operational accuracy
- Verify full functionality of all buttons, ensuring precise and responsive input control
- Check the XY assembly for proper movement and alignment; thoroughly clean all railings, removing any debris or contaminants
- Confirm stable software communication and connection between the instrument and PC, addressing any connectivity issues
- Inspect and clean or replace all filters as required to ensure optimal airflow and performance
- Examine internal wiring and electrical connections for any breaks, disconnections, or tears; replace or secure any compromised wiring
- Test the lamp’s full functionality, replacing it if any performance issues, dimming, or faults are detected
- Execute the necessary validation protocol, documenting completion with a date on a calibration/verification certificate
- Perform and verify appropriate updates to the PC operating system for compatibility and security
- Conduct a thorough cosmetic inspection, ensuring all external components meet quality and aesthetic standards
Technician Inspection and Recertification Checklist: Instruments
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
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Task
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Completed?
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Remarks?
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Confirm stable, correct voltage outputs from power supply and PCB
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Completed
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Inspect display for dim or dead pixels; replace as needed
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Completed
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Verify functionality of all buttons for precise, responsive control
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Completed
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Check XY assembly for proper movement; clean railings to remove debris
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Completed
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Confirm stable software communication between instrument and PC
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Completed
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Inspect and clean or replace filters to maintain optimal performance
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Completed
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Examine internal wiring for damage; replace or secure compromised connections
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Completed
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Test lamp functionality; replace if dimming or faults are detected
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Completed
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Execute validation protocol and document on calibration/verification certificate
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Completed
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Update PC operating system for compatibility and security
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Completed
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Conduct cosmetic inspection to ensure quality of external components
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Completed
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To View More of Our Recertification Protocol Click Here
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Fast and Quiet 
