Temperature

Range

4° to 100°C

Cycling Speed

96 x 0.2 ml Reaction Module: with 20 µl sample in a 0.2 ml tube in the 96 well module, after full warm up, when cycling between 50°C and 95°C; at least 3.3°C/sec maximum heating rate and 2.0°C/sec maximum cooling rate.

Storage Cooling

less than 3 minutes to cool from 40°C to 4°C

Overshoot

less than 0.5°C

Accuracy

±0.3°C to NIST traceable standard after 30 seconds at 90°C,

block mode

Uniformity

well-to-well uniformity of ±0.4°C within 30 seconds after

arrival at 90°C

Temperature Gradient

Input Gradient Range

1° to 25°C

Functional Gradient Range

5° to 25°C

Min Temp Gradient

40°C

Max Temp Gradient

99°C

Accuracy

±0.4°C

Uniformity

±0.4°C

Programming

Number of Programs

256 on board

Cycles/Protocol

up to 9

Steps/Cycle

up to 9

Repeats/Cycle

up to 600

Dwell Time

00:01 – 99:59 minutes

Pause

Yes

Built-In Protocols

Templates for cycle sequencing, Touchdown, RT PCR, nested PCR, long PCR