 |
 |
 |
|
Biorad Gradient 96 Well DNA Engine Thermal Cycler
The Biorad Gradient 96 Well DNA Engine Thermal Cycler utilizes interchangeable sample blocks and heat pump assemblies, which allow the user to change format easily between runs while retaining NIST-traceable accuracy. This thermal cycler's precise Peltier-effect heat pumps deliver a full temperature range of 0° to 100°C electronically. This compact instrument offers speedy ramping rates of up to 3.0/sec in models that hold 0.2ml tubes or V-well plates. The DNA Engine thermal cycler has unparalleled temperature homogeneity of ± 0.4°C within 30 seconds of arrival at 60°C for all standard models. This Bio-Rad machine offers a large memory and amiable software that allows control over virtually every thermal parameter.All of our equipment is fully tested to perform at factory specifications before being shipped.
The Biorad Gradient 96 Well DNA Engine Thermal Cycler includes:
- > 96 Well Alpha Block
- > Calibration and Preventative Maintenance Report
- > Operator's Manual
- > Power Cord
- > Full 180 Day Parts and Labor Warranty
Biorad Gradient 96 Well DNA Engine Thermal Cycler Specifications
|
Thermal Range
|
5 to 105°C, but no more than 30°C below ambient
temperature (4 to 100°C for the slide Chambers Alpha Unit)
|
|
Thermal Accuracy
|
± 0.3°C of programmed target at 90°C- NIST-traceable
|
|
Thermal Uniformity
|
± 0.4°C well-to-well within 30 seconds of arrival at 90°C (for most Alpha units; see specifications for individual
Alpha Units)
|
|
Parrallel
|
1x 25-pin 8-bit parallel interface printer port
|
|
Serial
|
1x 9-pin RS-232 serial port for printer or remote use
|
|
IEEE
|
one IEEE-488 bidirectional general purpose interface bus
|
|
Display
|
one 20 x 4 LCD alphanumeric display
|
|
W x L x H
|
12 x 12 x 12
|
|
Weight lbs
|
25
|
Biorad Gradient 96 Well DNA Engine Thermal Cycler Manual Click Here to View all the Technical Specs for the Biorad DNA Engine
- Validate that the power supply and PCB consistently deliver accurate, stable voltages across all circuits and connections, confirming operational reliability
- Thoroughly inspect the display for any dim, flickering, or dead pixels, replacing compromised segments to ensure clear visibility and precise data display
- Verify complete functionality and responsiveness of all buttons and knobs, confirming accurate and reliable input across all controls
- Ensure the machine completes all internal diagnostic checks with zero errors, indicating system readiness and functional integrity
- Confirm the machine accurately identifies attached blocks (96-well, dual 48, etc.), with seamless recognition across different configurations
- Conduct a detailed inspection of internal wiring and all electrical connections, checking for any signs of breaks, disconnections, or tears; replace or repair any underperforming components
- Examine the connector between the block and base for bent or broken pins, replacing as needed to maintain secure, reliable connections
- Calibrate well temperatures with a NIST-traceable thermometer, verifying accuracy and uniformity across all wells to meet specification standards
- Perform a comprehensive cosmetic inspection, ensuring all exterior surfaces and components are free of damage, wear, or defects, maintaining aesthetic and professional quality
Technician Inspection and Recertification Checklist: Thermal Cycler
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
|
Task
|
Completed?
|
Remarks?
|
|
Validate stable, accurate voltage outputs from power supply and PCB across circuits
|
Completed
|
|
|
Inspect display for dim, flickering, or dead pixels; replace as needed for clarity
|
Completed
|
|
|
Verify functionality and responsiveness of all buttons and knobs
|
Completed
|
|
|
Confirm machine completes internal diagnostics with zero errors
|
Completed
|
|
|
Ensure accurate block identification across configurations (96-well, dual 48, etc.)
|
Completed
|
|
|
Inspect internal wiring and connections for integrity; repair or replace as needed
|
Completed
|
|
|
Check connector between block and base for bent or broken pins; replace as necessary
|
Completed
|
|
|
Calibrate well temperatures with a NIST-traceable thermometer for accuracy
|
Completed
|
|
|
Conduct cosmetic inspection to ensure quality and professionalism of all surfaces
|
Completed
|
|
To View More of Our Recertification Protocol Click Here
|
|
|
 |
|
 |
|
