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BioTek Synergy LX Multi-Mode Microplate Reader (ABS/FLD/Lumi)
The BioTek Synergy LX Multi-Mode Microplate Reader (ABS/FLD/Lumi) economically automates many common microplate assays. The high-quality optical design ensures excellent data in absorbance, fluorescence, and luminescence detection modes. Absorbance optics include a xenon flash lamp and monochromator for continuous wavelength selection from the low UV through the visible range to 999 nm. Fluorescence and luminescence measurements are made with filter-based optics for optimal sensitivity and direct detection to prevent light loss resulting in outstanding accuracy. The broad wavelength ranges enable many common assays including nucleic acid and protein quantification, ELISA, BCA, Bradford and cell viability. Easily accessible assay specific filter cubes make running different assays quick and effortless, and with the Gen5 Software included, the Synergy LX is certainly a versatile assay workstation. The Gen5 software also allows for read modes including: endpoint, kinetic, area scanning, and absorbance spectral scanning, along with support for BioCell, Take3 Trio, and 60-, 72-, and 96-well Terasaki plates. Gen5 also offers extensive data analysis and reporting and exporting capabilities.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The BioTek Synergy LX Multi-Mode Microplate Reader (ABS/FLD/Lumi) includes:
- > Windows 11 Professional 64-Bit Data Station with Pre-Loaded Gen5 PC Software
- > Luminescence Filter Cube
- > 2 x Fluorescence Filter Cubes
- > ABS, FLD, LUM, and Self Test Calibration Report
- > Interface Cable
- > Operator's Manual
- > Full 180 Day Part and Labor Warranty
BioTek Synergy LX Multi-Mode Microplate Reader (ABS/FLD/Lumi) Specifications
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Absorbance Specifications
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Light Source
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Xenon flash lamp
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Detector
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Photodiode
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Wavelength Selection
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Monochromator
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Wavelength Range
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200 to 999 nm, in 1 nm increments
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Wavelength Bandwidth
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≤ 5 nm
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Wavelength Accuracy
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± 2 nm
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Wavelength Precision
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± 0.2 nm (standard deviation)
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Dynamic Range
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0 to 4.0 OD
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Photometric Resolution
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0.0001 OD (under Gen5 control)
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Pathlength Correction
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Yes (under Gen5 control)
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Photometric Accuracy
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<1% at 2.0 OD
<3% at 2.5 OD
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Photometric Linearity
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< 1% from 0 to 2.5 OD
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Photometric Repeatability
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< 0.5% at 2.0 OD
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Stray Light
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0.03% at 230 nm
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Reading Speed (Kinetic)
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96 wells: 12 seconds
384 wells: 23 seconds
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Fluorescence Specifications
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Light Source
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Halogen
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Detector
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PMT
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Wavelength Selection
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Bandpass filters
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Wavelength Range
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320 to 700 nm (low noise PMT)
320 to 850 nm (red-shifted PMT
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Dynamic Range
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> 6 decades
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Sensitivity
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Fluorescein 2 pM
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Reading Speed (Kinetic)
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96 wells: 24 seconds
384 wells: 76 seconds
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Luminescence Specifications
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Dynamic Range
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< 6 decades
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Sensitivity
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10 amol ATP
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General Specifications
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Microplate Formats
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6, 12, 24, 48, 96, 384 wells
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Read Methods
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End point, kinetic, area scanning, absorbance spectral scanning (under Gen5 control)
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Plate Shaking
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Linear, orbital, double-orbital
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Dimensions (H x W x D)
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12 x 15 x 15 in. (30.5 x 38.1 x 38.1 cm)
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Weight
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27 lb. (12.3 kg.)
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Electrical Requirements
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100-240V, 50/60Hz
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Computer Specifications
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Processor
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Intel Core I5-8350U CPU
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RAM
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16.0 GB
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Hard Drive
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512GB Internal SSD
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Operating System
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Windows 11 Professional, 64-bit
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- Confirm the power supply and PCB output correct, stable voltages across all required connections
- Inspect the display for any dim or dead pixels; replace affected components to maintain full visual clarity and operational accuracy
- Verify full functionality of all buttons, ensuring precise and responsive input control
- Check the XY assembly for proper movement and alignment; thoroughly clean all railings, removing any debris or contaminants
- Confirm stable software communication and connection between the instrument and PC, addressing any connectivity issues
- Inspect and clean or replace all filters as required to ensure optimal airflow and performance
- Examine internal wiring and electrical connections for any breaks, disconnections, or tears; replace or secure any compromised wiring
- Test the lamp’s full functionality, replacing it if any performance issues, dimming, or faults are detected
- Execute the necessary validation protocol, documenting completion with a date on a calibration/verification certificate
- Perform and verify appropriate updates to the PC operating system for compatibility and security
- Conduct a thorough cosmetic inspection, ensuring all external components meet quality and aesthetic standards
Technician Inspection and Recertification Checklist: Instruments
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
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Task
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Completed?
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Remarks?
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Confirm stable, correct voltage outputs from power supply and PCB
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Completed
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Inspect display for dim or dead pixels; replace as needed
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Completed
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Verify functionality of all buttons for precise, responsive control
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Completed
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Check XY assembly for proper movement; clean railings to remove debris
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Completed
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Confirm stable software communication between instrument and PC
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Completed
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Inspect and clean or replace filters to maintain optimal performance
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Completed
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Examine internal wiring for damage; replace or secure compromised connections
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Completed
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Test lamp functionality; replace if dimming or faults are detected
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Completed
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Execute validation protocol and document on calibration/verification certificate
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Completed
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Update PC operating system for compatibility and security
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Completed
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Conduct cosmetic inspection to ensure quality of external components
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Completed
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To View More of Our Recertification Protocol Click Here
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