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BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader
The BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader is a compact, advanced multi-detection system built with patented Hybrid Technology, melding monochromator-based and filter-based fluorescence detection systems. This unique combination offers unparalleled sensitivity and versatility, accommodating a wide array of microplate-based assays both for present and future requirements. Key features include a broad spectrum of detection modes such as Fluorescence Intensity, Time-Resolved Fluorescence, Luminescence, UV-Visible Absorbance, and more. Additionally, its modular and upgradeable architecture allows for cost-effective customization, ensuring it meets evolving laboratory needs.
At its core, the Synergy H4 houses a quadruple mono system with two double-grating monochromators, facilitating superior spectral scanning performance and flexibility. The variable bandpass selection further enhances the system's performance. On the other hand, the filter/mirror system of the Synergy H4 optimizes energy delivery to the sample, ensuring high signal-to-noise ratios, faster read speeds, and precise control over optical parameters. The unit's compatibility with the Take3 Plate also enables low-volume nucleic acid quantification, making the Synergy H4 an invaluable tool for achieving precise and efficient results in a variety of biochemical assays.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader includes:
- > Windows 11 Professional 64-Bit Data Station with Pre-Loaded Gen5 PC Software
- > 460 nm, 528 nm, 590 nm, and 645 nm Emission Filters
- > 340 nm, 460 nm, 485 nm, and 540 nm Excitation Filters
- > ABS, FLD, LUM, and Self Test Calibration Report
- > Interface Cable
- > Operator's Manual
- > Full 180 Day Part and Labor Warranty
BioTek Synergy H4 Hybrid Multi-Mode Microplate Reader Specifications
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Absorbance Specifications
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Light Source
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Xenon flash lamp
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Wavelength Selection
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Monochromator
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Wavelength Range
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230 to 999 nm, in 1 nm increments
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Bandpass
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2 nm (230 to 285 nm), 4 nm (> 285 nm)
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Dynamic Range
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0 to 4.0 OD
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Photometric Resolution
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0.0001 OD
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Pathlength Correction
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Yes
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Photometric Accuracy
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< 1% at 2.0 OD typical
< 3% at 3.0 OD typical
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Photometric Repeatability
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< 0.5% at 2.0 OD typical
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Fluorescence Intensity Specifications
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Light Source
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Tungsten halogen lamp
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Wavelength Selection
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Double grating monochromators (top/bottom) and deep blocking filters/dichroic mirrors (top)
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Wavelength Range
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Monochromators: 250 to 850 nm
Filters: 200 to 700 nm (850 nm option)
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Dynamic Range
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Monochromators: 5 decades
Filters/Mirrors: > 6 decades
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Sensitivity
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Top Monochromator: Fluorescein 2 pM typical (0.2 fmol/well 384-well plate)
Bottom Monochromator: Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate)
Filters/Mirrors: Fluorescein 1 pM typical (0.1 fmol/well 384-well plate)
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Luminescence Specifications
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Wavelength Range
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300 to 700 nm
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Dynamic Range
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< 6 decades
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Sensitivity
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10 amol ATP typical (flash)
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Fluorescence Polarization Specifications
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Light Source
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Tungsten halogen
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Wavelength Selection
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Deep blocking filters/dichroic mirrors (top)
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Wavelength Range
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400 to 700 nm (320 to 850 nm option)
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Sensitivity
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3 mP at 1 nm fluorescein typical
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Time-Resolved Fluorescence Specifications
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Light Source
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High energy xenon flash lamp
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Wavelength Range
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200 to 700 nm (850 nm option)
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Sensitivity
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Europium 60 fM typical with filters (6 amol/well in 384-well plate)
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Reagent Dispenser Specifications
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Number
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2 syringe pumps
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Dispense Volume
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5 to 1000 ul in 1 ul increments
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Minimum Prime Volume
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1.1 mL, 100 ul with back flush
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General Specifications
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Microplate Formats
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Monochromator System: 1 to 384-wells
Filter System: 1 to 1536-wells
PCR plates, and Take3 Micro-Volume Plates
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Read Methods
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End point, kinetic, spectral scanning, well area scanning
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Detection Methods
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FL, TRF, FP, Lum., UV-Vis Abs., AlphaScreen/AlphaLISA
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Plate Shaking
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Yes
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Temperature Control
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Ambient +4°C to 65°C, ± 0.5°C
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Dimensions (W x D x H)
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17 x 20.9 x 15 in. (43.5 x 53.1 x 38.1 cm)
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Weight
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78 lb. (35 kg.)
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Electrical Requirements
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100-240V, 50/60Hz
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Computer Specifications
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Processor
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Intel Core I5-8350U CPU
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RAM
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16.0 GB
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Hard Drive
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512GB Internal SSD
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Operating System
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Windows 11 Professional, 64-bit
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- Confirm the power supply and PCB output correct, stable voltages across all required connections
- Inspect the display for any dim or dead pixels; replace affected components to maintain full visual clarity and operational accuracy
- Verify full functionality of all buttons, ensuring precise and responsive input control
- Check the XY assembly for proper movement and alignment; thoroughly clean all railings, removing any debris or contaminants
- Confirm stable software communication and connection between the instrument and PC, addressing any connectivity issues
- Inspect and clean or replace all filters as required to ensure optimal airflow and performance
- Examine internal wiring and electrical connections for any breaks, disconnections, or tears; replace or secure any compromised wiring
- Test the lamp’s full functionality, replacing it if any performance issues, dimming, or faults are detected
- Execute the necessary validation protocol, documenting completion with a date on a calibration/verification certificate
- Perform and verify appropriate updates to the PC operating system for compatibility and security
- Conduct a thorough cosmetic inspection, ensuring all external components meet quality and aesthetic standards
Technician Inspection and Recertification Checklist: Instruments
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
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Task
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Completed?
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Remarks?
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Confirm stable, correct voltage outputs from power supply and PCB
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Completed
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Inspect display for dim or dead pixels; replace as needed
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Completed
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Verify functionality of all buttons for precise, responsive control
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Completed
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Check XY assembly for proper movement; clean railings to remove debris
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Completed
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Confirm stable software communication between instrument and PC
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Completed
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Inspect and clean or replace filters to maintain optimal performance
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Completed
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Examine internal wiring for damage; replace or secure compromised connections
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Completed
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Test lamp functionality; replace if dimming or faults are detected
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Completed
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Execute validation protocol and document on calibration/verification certificate
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Completed
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Update PC operating system for compatibility and security
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Completed
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Conduct cosmetic inspection to ensure quality of external components
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Completed
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To View More of Our Recertification Protocol Click Here
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