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BioTek ELx405U Microplate Washer
The BioTek ELX405U Microplate Washer is designed specifically to rapidly wash 96 or 384-well microplates and includes BioTek's patented Dual-Action™ manifold to ensure optimal performance and easy maintenance. The ELX405U microplate washer comes standard with Bio-Tek's unique AutoPrime and AutoRinse functions for reliable performance. Programming is made easy through simple keypad interface with independent control of plate position during dispense and aspiration cycles, as well as fully programmable fluid volumes and flow rates, shaking, and soaking. Easy maintenance with a selection of pre-programmed routines for problem-free operation and run protection via fluid detection, fluid flow, and vacuum sensors. All of our equipment is fully tested to perform at factory specifications before being shipped.
The BioTek ELX405U Microplate Washer includes:
- > (2) 4 Liter Waste Bottles
- > (1) 4 Liter Buffer Bottle
- > Vacuum Pump
- > Stainless Steel Bottle Holder
- > Microplate Carrier
- > Vacuum Tubing with Connections
- > Power Cord
- > Operator's Manual
- > Full 180 Day Parts and Labor Warranty
BioTek ELx405U Microplate Washer Specifications
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Microplate Types
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96 and 384 well plates
Low profile and standard height
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Onboard Software
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Create, edit and run multiple protocols
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Soaking
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Programmable up to 60 minutes
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Manifold Types
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96 Tube Dual Action manifold for 96 and 384 well washing
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Volume Range
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50 to 3000 microliter increments
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Wash Cycles
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1 -10
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Buffer/reagent Selection
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Auto switching module for up to 4 buffers (option)
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Supply bottle
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4 L
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Dispense Precision
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≤3% CV: 300 µL/well (96-well washing)
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Residual Volume
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<2 µL/well (standard 96-well plates)
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Wash Speed
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96-wells, 300 µL/well, 3 cycles: <30 seconds
384-wells,100 µL/well, 3 cycles: <80 seconds
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Flow Rates
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High flow to low flow
Optimized rates for cell assays
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Sterilization
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Chemical
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Vacuum Filtration
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Selectable: Approximate final at 30 seconds:
Lowest: -38 mmHg
Low: -113 mmHg
Medium: -200 mmHg
High: -390 mmHg
Highest: -506 mmHg
Vacuum filtration time range: 5 to 999 seconds
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Operator's Manual
 Click Here to View all the Technical Specs for the ELx405U
Click Here for an Overview Comparision of Biotek ELX405 Microplate Washer Models
- Install new waste and buffer bottles to ensure proper fluid containment and system functionality.
- Integrate a new pump into the system, verifying compatibility and secure connections.
- Verify that the power supply and PCB output the correct voltages for reliable component operation.
- Conduct a self-test on the microplate washer, evaluating all operational parameters.
- Inspect the X-axis motor belt for signs of wear, such as tears, frays, or breakages, replacing if necessary.
- Examine dispense and aspirate tubes for any damage that could affect fluid flow accuracy.
- Confirm internal pump functionality, replacing seals as needed to maintain optimal performance.
- Inspect all internal wiring for frays, tears, or breakages, addressing any faults for electrical safety.
- Verify functionality of all internal and external sensors, ensuring accurate readings across the unit.
- Disinfect the unit using an appropriate disinfection cycle to ensure sterility and safety.
- Check that residual aspiration levels are within the manufacturer’s specified limits for accuracy.
- Conduct a final cosmetic inspection to verify that all external surfaces meet appearance standards.
Technician Inspection and Recertification Checklist: Microplate Washer
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
| Task |
Completed? |
Remarks? |
| Install new waste and buffer bottles | Completed | |
| Integrate new pump into system, verifying secure connections | Completed | |
| Verify power supply and PCB output correct voltages | Completed | |
| Conduct microplate washer self-test and evaluate results | Completed | |
| Inspect X-axis motor belt for wear, replacing if necessary | Completed | |
| Examine dispense and aspirate tubes for any damage | Completed | |
| Confirm internal pump functionality, replace seals as needed | Completed | |
| Inspect internal wiring for frays, tears, or breakages | Completed | |
| Verify functionality of all internal and external sensors | Completed | |
| Disinfect unit with appropriate disinfection cycle | Completed | |
| Check residual aspiration levels meet specifications | Completed | |
| Conduct cosmetic inspection for appearance standards | Completed | |
To View More of Our Recertification Protocol Click Here
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