BD Bioscience FacsCanto II Flow Cytometer
The BD Bioscience FACSCanto II Flow Cytometer is a benchtop analyzer
providing reliable and accurate results for experiments with up to 10
parameters. The FacsCanto II offers unbeatable consistency and optimal signal detection with increased resolution and sensitivity for multicolor assays.
The fluidics system features a fixed-alignment flow cell to
minimize startup time and improve reproducibility. The BD FACSCanto II fluidics
system is designed to streamline work, save time, and improve performance
through the single-file flow cell streaming, a new dynamic feedback pressure
control system designed to regulate pressure, and hydrodynamic focusing. These features allow particles to flow through the center of the flow cell, and by increasing the sample pressure, the core diameter and flow rate will also increase. The fluidics cart allows for a consistent level of fluid within the sheath cubitainer, while also removing small air bubbles from the supply.
The excitation and collection design for the BD FACSCanto II
optics system reduces losses, allowing for more information collection from
each sample. This system consists of an excitation source with two lasers: blue (488-nm, air-cooled, 20-mW solid state) and red (633-nm, 17-mW HeNe). The excitation optics consist of
multiple fixed-wavelength lasers, fiber optics up to the beam-shaping prisms,
and achromatic focusing lenses that produce spatially separated beam spots in
the flow cell.
The BD Bioscience FACSCanto II Flow Cytometer supports a
broad range of research applications, being particularly suited for rare event
analysis with its high speed, great sensitivity, and minimal sample-to-sample
carryover.
All of our equipment is fully tested to perform at factory
specifications before being shipped.
Included with the BD Bioscience FacsCanto II Flow Cytometer:
Includes:
> Full 180 Day Parts and Labor Warranty
BD Bioscience FacsCanto II Flow Cytometer Specifications
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Cytometer
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Dimensions (W x H x D)
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91 x 64 x 61 cm (35.7 x 25.2 x 24 in)
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Workspace Dimensions
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Height (with flow cell access door open): 85 cm (33.5 in)
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Power Requirements
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100/115/230 VAC (50-60 Hz)
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Fluorescence Threshold Sensitivities
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FITC < 100 MESF
PE < 50 MESF
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Forward and Side Scatter Sensitivities
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Platelets can be resolved from noise
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Forward Scatter Sensitivity
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1 micron
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Side Scatter Sensitivity
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0.5 micron
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Data Acquisition Channels
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8 to 10 parameters: 6 to 8 fluorescent and 2 scatter parameters
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Fluidics Cart
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Dimensions (W x H x L)
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79 x 64 x 61 cm (31.1 x 25.2 x 24 in)
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BD FACSFlow Cubitainer Capacity
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20 L
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BD FACSClean Solution Cubitainer Capacity
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5 L
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BD FACS Shutdown Solution Cubitainer Capacity
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5 L
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Waste Tank Capacity
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10 L
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Fluidics
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General Operation
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Integrated fluidics cart with automated startup, shutdown, and
cleaning cycles
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Sheath Consumption
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1.1
L/hr, normal operation
< 1.0 mL/hr, standby
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Sample Flow Rates
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Assay dependent, controlled automatically by BD FACSCanto clinical
software. Nominal rates:
Low = 10 µL/min
Medium = 60 µL/min
High = 120 µL/min
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Sample Acquisition Rate
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10,000 events/sec with <10% abort rate (8 parameters)
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Recommended Max Particle Size
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50 µm
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Signal Processing
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Workstation Resolution
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262,144-channel resolution
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Data Acquisition Channels
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8 to 10 parameters: 6 to 8 fluorescent and 2 scatter parameters
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Fluorescence Compensation
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No limit to inter- and intra-beam compensation
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Pulse Processing
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Height, area, and width measurements available for any parameter (BD
FACSDiva software)
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Time
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Can be correlated to any parameter
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Channel Threshold
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Available for any parameter from all lasers
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Reference Manual
Excellent Condition 
