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Applied Biosystems QuantStudio 7 Flex Real-Time PCR System
Ramp-Down Rate
3.0°C/second
Well-to-Well Variability
± 0.25°C
- Advanced multiplexing capabilities with interchangeable block formats for diverse applications
- Accommodates four different block types including 96-well, 96-well Fast, 384-well, and TaqMan Array Card (384-well block included)
- Compatible with over 8 million TaqMan assays for comprehensive experimental flexibility
- Simplified workflow with intuitive software and touch-screen interface
- Tool-free block exchange enhances ease of use and operational efficiency
- OptiFlex system features six decoupled excitation and emission filter channels with 21 filter combinations
- Detects changes in target quantity as small as 1.5-fold in single-plex reactions
- Offers a linear dynamic range of 10 logs for precise quantification
- Designed for high throughput with automation capabilities for streamlined processing
- Supports a wide range of applications, including gene expression, SNP genotyping, and protein thermal shift experiments
The Applied Biosystems QuantStudio 7 Flex Real-Time PCR System represents a significant advancement in PCR technology, offering extensive multiplexing capabilities and interchangeable block formats to support a wide array of real-time PCR applications. This system is designed to enhance research flexibility and precision, accommodating four different block types: 96-well, 96-well Fast, 384-well, and TaqMan Array Card blocks. The inclusion of over 8 million compatible TaqMan assays for diverse experiments, such as gene expression and protein expression, underscores its application versatility. Moreover, the system's intuitive touch-screen interface, combined with automation-friendly features and tool-free block exchange, simplifies workflows and promotes efficiency in the laboratory setting.
Key to the QuantStudio 7 Flex system's reliability are its advanced optical and thermal components, which ensure minimal variability both within and across instruments. The OptiFlex system, which consists of six independent excitation and emission filter channels with 21 filter combinations, allows for high flexibility in multiplexing and chemical reactions. This system is particularly effective in detecting subtle changes in target quantity, achieving a linear dynamic range over ten logs and facilitating sensitive data analysis in single-plex reactions.
For laboratories aiming to streamline their processes and increase throughput, the QuantStudio 7 Flex system is an ideal solution. It supports a variety of applications from gene regulation studies to protein thermal shift assays. Its high ramp-up and ramp-down rates of 3.5°C/second and 3.0°C/second respectively, along with low well-to-well variability (± 0.25°C), ensure rapid and consistent performance. Additionally, the system is designed for easy integration with robotics for automated setups, making it a powerful tool for high-throughput environments where precision and reliability are paramount. Please Note: We recommend performing a Dye Calibration for the Dye you plan on using for your application(s). Please refer to the Operator's Manual for instructions.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The Applied Biosystems QuantStudio 7 Flex Real-Time PCR System includes:
- > 384-Well Block
- > 384-Well Heated Cover
- > New Lamp
- > Background Calibration Report
- > ROI Calibration Report
- > Uniformity Calibration Report
- > Windows 11 Professional 64-Bit Data Station with Flatscreen Monitor
- > QuantStudio Real-Time PCR Software Version 1.7.2 Installed
- > Power Cord
- > Operator's Manual
- > Full 180 Day Parts and Labor Warranty
Applied Biosystems QuantStudio 7 Flex Real-Time PCR System Specifications
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Block Configuration
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384-well
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Block Change Design
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Block change from front in less than 1 minute; no tools required
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Ramp-Up Rate
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3.5°C/second
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Ramp-Down Rate
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3.0°C/second
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Well-to-Well Variability
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± 0.25°C
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Excitation Source
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OptiFlex system lamp
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Detection Channels
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Excitation: 455 to 672 nm
Emission: 505 to 723 nm
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Dimensions (H x W x D)
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11.8 x 9.9 x 3.1 in. (73.8 x 50.1 x 66.1 cm)
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Weight
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154.3 lb. (70 kg)
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Electrical Requirements
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100-240V, 50/60Hz, 12.5A
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Computer Specifications
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Processor
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Intel Core I5-8500 CPU
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RAM
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32.0 GB
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Hard Drive
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500GB Internal SSD
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Operating System
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Windows 11 Professional, 64-bit
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- Validate that the power supply and PCB consistently deliver accurate, stable voltages across all circuits and connections, confirming operational reliability
- Thoroughly inspect the display for any dim, flickering, or dead pixels, replacing compromised segments to ensure clear visibility and precise data display
- Verify complete functionality and responsiveness of all buttons and knobs, confirming accurate and reliable input across all controls
- Ensure the machine completes all internal diagnostic checks with zero errors, indicating system readiness and functional integrity
- Confirm the machine accurately identifies attached blocks (96-well, dual 48, etc.), with seamless recognition across different configurations
- Conduct a detailed inspection of internal wiring and all electrical connections, checking for any signs of breaks, disconnections, or tears; replace or repair any underperforming components
- Examine the connector between the block and base for bent or broken pins, replacing as needed to maintain secure, reliable connections
- Calibrate well temperatures with a NIST-traceable thermometer, verifying accuracy and uniformity across all wells to meet specification standards
- Perform a comprehensive cosmetic inspection, ensuring all exterior surfaces and components are free of damage, wear, or defects, maintaining aesthetic and professional quality
Technician Inspection and Recertification Checklist: Thermal Cycler
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
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Task
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Completed?
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Remarks?
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Validate stable, accurate voltage outputs from power supply and PCB across circuits
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Completed
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Inspect display for dim, flickering, or dead pixels; replace as needed for clarity
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Completed
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Verify functionality and responsiveness of all buttons and knobs
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Completed
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Confirm machine completes internal diagnostics with zero errors
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Completed
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Ensure accurate block identification across configurations (96-well, dual 48, etc.)
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Completed
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Inspect internal wiring and connections for integrity; repair or replace as needed
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Completed
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Check connector between block and base for bent or broken pins; replace as necessary
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Completed
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Calibrate well temperatures with a NIST-traceable thermometer for accuracy
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Completed
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Conduct cosmetic inspection to ensure quality and professionalism of all surfaces
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Completed
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To View More of Our Recertification Protocol Click Here
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