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Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 384-Well Sample Block
Block Configuration
384-Well (5 to 20 uL reactions)
Maximum Ramp Rate
6.0°C/second
Temperature Uniformity
± 0.4°C
- Average sample ramp rate of 2.92°C/second for efficient operations
- Equipped with bright white LED and five coupled filters for a broad range of detection (450 to 650 nm excitation and 500 to 700 nm detection)
- Allows multiplexing of up to five targets, enhancing assay capability
- Dynamic range over 10 logarithmic units for excellent sensitivity and precision
- Onboard memory capacity of 10 GB, storing between 2,000 to 5,000 run files
- Desktop and web-based software supports 21 CFR Part 11 compliance for security and e-signature
- Integrated with Connect cloud-based platform for remote data access and management
- Designed for quick installation and immediate use, facilitating seamless integration into lab workflows
- Features pre-optimized protocol templates to minimize training requirements for new users
- Offers locked workflow options to ensure experimental consistency across runs
The Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 384-Well Sample Block offers high-performance, adaptable configurations suitable for any laboratory setting. It supports sample reactions ranging from 5 to 20 µL and achieves rapid thermal cycling with a maximum ramp rate of 6.0°C/second, along with an average sample ramp rate of 2.92°C/second. The system ensures precise temperature uniformity (± 0.4°C), essential for consistent results. Featuring a bright white LED excitation source and five coupled filters, the QuantStudio 5 spans an excitation range of 450 to 650 nm and a detection range of 500 to 700 nm, enabling efficient multiplexing of up to five targets simultaneously.
With a dynamic range that extends over 10 logarithmic units, this system is capable of detecting subtle differences as small as 1.5-fold in target quantities in singleplex reactions, offering superior sensitivity and resolution. The onboard memory supports up to 10 GB, accommodating between 2,000 to 5,000 run files, which simplifies data management and accessibility.
This system also features comprehensive software that includes desktop and web-based analysis options. The software supports critical security measures, including auditing, e-signature capabilities, and compliance with 21 CFR Part 11, ensuring that your experimental data and run information are securely managed. Additionally, the QuantStudio 5 integrates seamlessly with the Connect cloud-based platform, allowing for remote data access and management anytime and anywhere, enhancing the convenience and efficiency of your research operations. Please Note: We recommend performing a Dye Calibration for the Dye you plan on using for your application(s). Please refer to the Operator's Manual for instructions.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 384-Well Sample Block includes:
- > 384-Well Block
- > Background Calibration Report
- > ROI Calibration Report
- > Uniformity Calibration Report
- > Windows 11 Professional 64-Bit Data Station with Flatscreen Monitor
- > QuantStudio Design & Analysis Desktop Software Installed
- > Power Cord
- > Operator's Manual
- > Full 180 Day Parts and Labor Warranty
Applied Biosystems QuantStudio 5 Real-Time PCR System w/ 384-Well Sample Block Specifications
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Block Configuration
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384-Well (5 to 20 uL reactions)
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Maximum Ramp Rate
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6.0°C/second
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Average Sample Ramp Rate
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2.92°C/second
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Temperature Uniformity
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± 0.4°C
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Run Time
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Less than 30 minutes
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Heating and Cooling Method
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Peltier
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Excitation (Light Source)
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Bright white LED
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Filters or Colors
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5 coupled filters
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Excitation and Detection Range
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450 to 650 nm and 500 to 700 nm
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Multiplexing
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Up to 5 targets
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Dynamic Range
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10 logarithmic units
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Sensitivity (Resolution)
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Detect differences as small as 1.5-fold in target quantities in a singleplex reaction
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Data Acquisition
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Whole-plate imaging
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Onboard Memory
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10 GB, which translates to approximately 2,000 to 5,000 run files
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Dye Compatibility
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FAM™/SYBR™ Green, VIC™/JOE™/HEX™/TET™, ABY™/NED™/TAMRA™/Cy®3, JUN™, ROX™/Texas Red™, Mustang Purple™, Cy®5/LIZ™, Cy®5.5
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Dimensions (W x D x H)
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10.6 x 19.7 x 15.7 in. (27 x 50 x 40 cm)
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Weight
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57.3 lb. (26 kg)
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Electrical Requirements
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100-240V, 50/60Hz
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Computer Specifications
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Processor
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Intel Core I5-8350U CPU
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RAM
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16.0 GB
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Hard Drive
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512GB Internal SSD
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Operating System
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Windows 11 Professional, 64-bit
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- Validate that the power supply and PCB consistently deliver accurate, stable voltages across all circuits and connections, confirming operational reliability
- Thoroughly inspect the display for any dim, flickering, or dead pixels, replacing compromised segments to ensure clear visibility and precise data display
- Verify complete functionality and responsiveness of all buttons and knobs, confirming accurate and reliable input across all controls
- Ensure the machine completes all internal diagnostic checks with zero errors, indicating system readiness and functional integrity
- Confirm the machine accurately identifies attached blocks (96-well, dual 48, etc.), with seamless recognition across different configurations
- Conduct a detailed inspection of internal wiring and all electrical connections, checking for any signs of breaks, disconnections, or tears; replace or repair any underperforming components
- Examine the connector between the block and base for bent or broken pins, replacing as needed to maintain secure, reliable connections
- Calibrate well temperatures with a NIST-traceable thermometer, verifying accuracy and uniformity across all wells to meet specification standards
- Perform a comprehensive cosmetic inspection, ensuring all exterior surfaces and components are free of damage, wear, or defects, maintaining aesthetic and professional quality
Technician Inspection and Recertification Checklist: Thermal Cycler
The Following Checklist Is to Be Performed on All Incoming and Outgoing Pieces of Equipment
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Task
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Completed?
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Remarks?
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Validate stable, accurate voltage outputs from power supply and PCB across circuits
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Completed
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Inspect display for dim, flickering, or dead pixels; replace as needed for clarity
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Completed
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Verify functionality and responsiveness of all buttons and knobs
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Completed
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Confirm machine completes internal diagnostics with zero errors
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Completed
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Ensure accurate block identification across configurations (96-well, dual 48, etc.)
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Completed
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Inspect internal wiring and connections for integrity; repair or replace as needed
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Completed
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Check connector between block and base for bent or broken pins; replace as necessary
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Completed
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Calibrate well temperatures with a NIST-traceable thermometer for accuracy
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Completed
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Conduct cosmetic inspection to ensure quality and professionalism of all surfaces
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Completed
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To View More of Our Recertification Protocol Click Here
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