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Amersham Biosciences AKTA Purifier 100 FPLC System w/ UV-900 Detector
Amersham Biosciences AKTA Purifier 100 FPLC System w/ UV-900 Detector
 
 



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Condition: Fully Recertified and Tested to Perform at Manufacturer Specifications
Warranty: 180-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 3 to 5 Business Days
Included Documentation: Installation Calibration Report

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Recertification Protocol
Technical Specification Sheet
 

Amersham Biosciences AKTA Purifier 100 FPLC System w/ UV-900 Detector


The Amersham Biosciences AKTA Purifier 10 FPLC System w/ UV-900 Detector is a versatile, modular liquid chromatography systems for fast and reliable separations of proteins, peptides, and nucleic acids by FPLC. The AKTA Purifier 100 is designed for purification of microgram to milligram quantities of protein, operating at flow rates of 0.01 to 100 ml/min at pressures of 0 to 10 MPa. The Amersham Biosciences AKTA is in excellent condition and has passed all tests for full functionality.

These tests included: linking the unit to the Unicorn 5.31 operating system, testing flow rate accuracy of P-900 Pump, testing PHC-900 Monitor with Nal test solution, testing pH/C-900 for functional communication with pH probe and Conductivity Cell, testing motor valves, and testing the Frac-920 for functionality through manual commands. (Additional cost for pH Probe and Holder, please inquire).

INCLUDED:
The Amersham Biosciences AKTA Purifier 100 FPLC System w/ UV-900 Detector includes:
  • > Box-900
  • > P-900 Pump w/ 100ml Pump Heads
  • > P-960 Sample Pump
  • > UV-900 Detector
  • > PHC-900 Monitor
  • > INV-907 Valve
  • > PV-908 Valves (2)
  • > M-925 Mixer w/ 2ml Mixing Chamber
  • > Conductivity Flow Cell
  • > CU-950 Communication Module
  • > Frac-920 Fraction Collector with Tube Rack
  • > Dell Windows 7 Datastation with installed Unicorn version 5.31 software
  • > Flat Screen Monitor
  • > Installation Calibration Report
  • > Communication Cables
  • > Tubing, Power Cords
  • > Full 180 Day Parts & Labor Warranty

Amersham Biosciences AKTA Purifier 100 FPLC System w/ UV-900 Detector Specifications

P-900 Specifications

Flow Rate Range

Isocratic mode: 0.01 to 100 ml/min in steps of 10 ul/min

Gradient mode: 0.01 to 100 ml/min in steps of 10 ul/min

Double mode: 0.01 to 200 ml/min in steps of 10 ul/min

Pressure Range

0 to 10 MPa (100 bar, 1450 psi)

pH Stability Range

1 to 13 (1 to 14 with <1 day exposure)

Viscosity

Max 5 cP

Flow Rate Accuracy

± 2% or 20 ul/min, whichever is greater

Flow Rate Reproducibility

Flow rate ≥ 0.5 ml/min: rsd < 0.5%

Gradient Composition

Accuracy: < ±1% at 0.05 to 10 ml/min

Reproducibility: rsd < 0.25% at 0.5 to 100 ml/min

Pressure Sensor

Range: 0 to 27.5 MPa

Offset error: < 0.05 MPa

Scale error: < ±2%

Total Volume Between Inlet and Outlet

< 800 ul/module

UV-900 Specifications

Wavelength range

190–700 nm in steps of 1 nm,

3 wavelengths simultaneously

Bandwidth

4 nm

Wavelength accuracy

±2 nm

Wavelength reproducibility

< 500 ms (one cycle from 214 nm to

254 nm and back to 214 nm)

Linearity

< 2% deviation up to 2 AU at 260 nm with

Uracil at pH 2

Static noise

short term: < 6x10-5 AU at 230 nm

long term: < 6x10-5 AU at 230 nm

Drift

< 2x10-4 AU/h at 254 nm

M-925 Specifications

Max Flow Rate

0.001 to 10 ml/min

Max Pressure

12ml chamber: 10 MPa at 0.1 to 100 ml/min (100 bar, 1450 psi)

0.6, 2, and 5ml chambers: 25 MPa at 0.001 to 100 ml/min (250 bar, 3625 psi)

90 ul and 0.2 ml chambers: 35 MPa at 0.001 to 5 ml/min (250 bar, 5075 psi)

pH Stability Range

1 to 13 (1 to 14 with <1 day exposure)

Viscosity

Max 5 cP

Internal Volume

6 interchangeable mixing chambers: 90 ul, 0.2, 0.6, 2, 5, and 12 ml

Mixing Principle

1 static chamber and 1 dynamic chamber

Inlet and Outlet Tubing

UNF 10 to 32 thread profile

Frac-920 Specifications

Capacity

95 fractions in 10-18 mm tubes

Depth

400 mm

Flow Rate

< 100 ml/min

Height

250 mm

Operating Temperature

4° to 40°C

pH Stability Long Term

1 – 13

pH Stability Short Term

1 – 14

Program Memory

99 programs

Rack Designation

95 x 10-18 mm (Standard)

175 x 12 mm and 40 x 30 mm (Optional)

Relative Humidity 20 to 95% (non condensing)
Time Mode 0.01 to 9.999 min
Volume Mode 0.1 to 999 ml

Computer Specifications

Model

Dell Optiplex 7010 Desktop PC

Processor

Intel Core i5-3470 Processor 3.2GHz

RAM

8GB

Hard Drive

250GB Internal SSD

Operating System

Windows 7 Professional, 64-bit

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Brand
Brand > Amersham Biosciences