 |
 |
 |
|
GE AKTA Purifier 100 FPLC System w/ UPC-900
- Flow Rate of 0.01 to 100 ml/min
- Pressure Range of 0 to 10 MPa
- Absorbance Range of 0.01 to 5 AU
- Full 180 Day Parts and Labor Warranty
Go to Product Details
Amersham Biosciences AKTA Purifier 100 FPLC System w/ UPC-900
The Amersham Biosciences AKTA Purifier 100 FPLC System is a versatile, modular liquid chromatography system for fast and reliable separations of proteins, peptides, and nucleic acids by FPLC. The A KTA Purifier 100 is designed for purification of microgram to milligram quantities of protein, operating at flow rates of 0.01 to 100 ml/min at pressures of 0 to 10 MPa. The Amersham Biosciences AKTA is in excellent condition and has passed all tests for full functionality. These tests included: linking the unit to the Unicorn 5.31 operating system, testing flow rate accuracy of P-900 Pump, testing UPC-900 Monitor with Nal test solution, testing pH/C-900 for functional communication with pH probe and Conductivity Cell, testing motor valves, and testing the Frac-920 for functionality through manual commands. (Additional cost for pH Probe and Holder, please inquire).
The Amersham Biosciences AKTA Purifier 100 FPLC System w/ UPC-900 includes:
- > Box-900
- > P-900 Pump w/ 100ml Pump Heads
- > UPC-900 Monitor
- > INV-907 Valve
- > M-925 Mixer w/ 2ml Mixing Chamber
- > Conductivity Flow Cell
- > CU-950 Communication Module
- > Frac-920 Fraction Collector with Tube Rack
- > Dell Windows 7 Datastation with installed Unicorn version 5.31 software
- > Flat Screen Monitor
- > Installation Calibration Reports
- > Communication Cables
- > Tubing, Communication Cables, Power Cords
- > Full 180 Day Parts & Labor Warranty
Amersham Biosciences AKTA Purifier 100 FPLC System w/ UPC-900 Specifications
|
P-900
Specifications
|
|
Flow Rate Range
|
Isocratic mode: 0.01 to 100 ml/min in steps of 10 ul/min
Gradient mode: 0.01 to 100 ml/min in steps of 10 ul/min
Double mode: 0.01 to 200 ml/min in steps of 10 ul/min
|
|
Pressure Range
|
0 to 10 MPa (100 bar, 1450 psi)
|
|
pH Stability Range
|
1 to 13 (1 to 14 with <1 day exposure)
|
|
Viscosity
|
Max 5 cP
|
|
Flow Rate Accuracy
|
± 2% or 20 ul/min, whichever is greater
|
|
Flow Rate Reproducibility
|
Flow rate ≥ 0.5 ml/min: rsd < 0.5%
|
|
Gradient Composition
|
Accuracy: < ±1% at 0.05 to 10 ml/min
Reproducibility: rsd < 0.25% at 0.5 to 100 ml/min
|
|
Pressure Sensor
|
Range: 0 to 27.5 MPa
Offset error: < 0.05 MPa
Scale error: < ±2%
|
|
Total Volume Between Inlet and Outlet
|
< 800 ul/module
|
|
UPC-900 Specifications
UV measurement
|
|
Absorbance range
|
0.01 - 5 AU (full scale)
|
|
Autozero range
|
-0.2 - 2.0 AU
|
|
Baseline adjust Adjustable
|
0-100% of full scale
|
|
Wavelengths
|
Hg lamp, fixed: 254 and 280
by changing filter:313, 365, 405, 436 and 546 nm
Zn lamp: 214 nm
|
|
Linearity, 5 mm cell
|
<3% up to 2 AU at 254 nm
<5% up to 1 AU at 280 nm
|
|
Static noise
|
short term: 40x10-6 AU at 254 nm
(typically 6x10-6 AU at 254 nm)
long term: 40x10-6 AU at 254 nm
|
|
Static Drift
|
±100x10-6 AU/h at 254 nm
|
|
Flow Sensitivity
|
2x10-4 AU min/ml
|
|
M-925
Specifications
|
|
Max Flow Rate
|
0.01 to 10 ml/min
|
|
Max Pressure
|
12ml chamber: 10 MPa at 0.1 to 100 ml/min (100 bar, 1450 psi)
0.6, 2, and 5ml chambers: 25 MPa at 0.01 to 100 ml/min (250 bar,
3625 psi)
90 ul and 0.2 ml chambers: 35 MPa at 0.01 to 5 ml/min (250 bar, 5075
psi)
|
|
pH Stability Range
|
1 to 13 (1 to 14 with <1 day exposure)
|
|
Viscosity
|
Max 5 cP
|
|
Internal Volume
|
6 interchangeable mixing chambers: 90 ul, 0.2, 0.6, 2, 5, and 12 ml
|
|
Mixing Principle
|
1 static chamber and 1 dynamic chamber
|
|
Inlet and Outlet Tubing
|
UNF 10 to 32 thread profile
|
|
Frac-920
Specifications
|
|
Capacity
|
95 fractions in 10-18 mm tubes
|
|
Depth
|
400 mm
|
|
Flow Rate
|
< 100 ml/min
|
|
Height
|
250 mm
|
|
Operating Temperature
|
4° to 40°C
|
|
pH Stability Long Term
|
1 – 13
|
|
pH Stability Short Term
|
1 – 14
|
|
Program Memory
|
99 programs
|
|
Rack Designation
|
95 x 10-18 mm (Standard)
175 x 12 mm and 40 x 30 mm (Optional)
|
|
Relative Humidity
|
20 to 95% (non condensing)
|
|
Time Mode
|
0.01 to 9.999 min
|
|
Volume Mode
|
0.1 to 999 ml
|
|
Computer Specifications
|
|
Model
|
Dell Optiplex 7010 Desktop PC
|
|
Processor
|
Intel Core i5-3470 Processor 3.2GHz
|
|
RAM
|
8GB
|
|
Hard Drive
|
250GB Internal SSD
|
|
Operating System
|
Windows 7 Professional, 64-bit
|
- Inspect all displays, replacing any dim or dead pixels to ensure optimal visual feedback
- Examine internal components of P-900, UV-900/UPC-900, and pH/C-900, replacing any malfunctioning parts to maintain performance
- Verify connections of all components via Uni-Net 1 & 2 interfaces for secure and reliable communication
- Check Uni-Net cables, replacing any faulty ones to ensure uninterrupted data transmission
- Inspect internal components of M-925, INV-907, PV-908, and PV-907 to confirm full operational functionality
- Replace all PEEK tubing with new OEM tubing and inspect all components within the flow path for potential clogs
- Confirm successful software interfacing with the FPLC system for seamless operation
- Replace pump piston seals, wash seals, and seal wash tubing, performing ultrasonic cleaning on each check valve in the pump flow path
- Calibrate pressure and flow rates of both the P-900 pump and P-960/950 pump (if applicable) for precise control
- Replace UV lamp and perform internal inspection tests to confirm complete functionality
- Install a new pH probe and verify the conductivity cell for accurate readings
- Calculate and adjust delay volume in software to reflect system configuration
- Clean or replace the drop sensor, accumulator wash, and height adjuster for consistent collection performance
- Verify fraction collector alignment and calibration with 15mL, 50mL, and microplate receptacles to ensure accuracy
- Run installation tests and thoroughly document all findings for comprehensive record-keeping
Technician Inspection and Recertification Checklist: FPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
|
Task
|
Completed?
|
Remarks?
|
|
Inspect all displays and replace dim/dead pixels
|
Completed
|
|
|
Examine internal components of P-900, UV-900/UPC-900, and pH/C-900
|
Completed
|
|
|
Verify connections via Uni-Net 1 & 2 interfaces
|
Completed
|
|
|
Check and replace any malfunctioning Uni-Net cables
|
Completed
|
|
|
Inspect internal components of M-925, INV-907, PV-908, PV-907
|
Completed
|
|
|
Replace PEEK tubing with OEM tubing and check for clogs
|
Completed
|
|
|
Confirm software interfacing with FPLC system
|
Completed
|
|
|
Replace pump seals and wash tubing; clean check valves
|
Completed
|
|
|
Calibrate pressure and flow rates of P-900 and P-960/950 pumps
|
Completed
|
|
|
Replace UV lamp and confirm full functionality
|
Completed
|
|
|
Install new pH probe and verify conductivity cell
|
Completed
|
|
|
Calculate delay volume and update in software
|
Completed
|
|
|
Clean or replace drop sensor, accumulator wash, and height adjuster
|
Completed
|
|
|
Verify fraction collector alignment with 15mL, 50mL, and microplate receptacles
|
Completed
|
|
|
Run installation tests and document all findings
|
Completed
|
|
To View More of Our Recertification Protocol Click Here
|
|
|
 |
|
 |
|
