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Amersham Biosciences AKTA Purifier 10 FPLC System
Flow Rate Range
0.001 to 10 ml/min
Pressure Range
0 to 25 Mpa
Absorbance Range
0.01 - 5 AU (full scale)
- Versatile and modular liquid chromatography system for protein, peptide, and nucleic acid separations
- Designed for purification of microgram to milligram quantities of protein
- In excellent condition and fully tested for full functionality
- Comes with Unicorn 5.31 operating system for seamless control
- Precision flow rate accuracy and testing of P-900 Pump for reliable performance
- Includes UPC-900 Monitor
- Functional communication with pH probe and Conductivity Cell for comprehensive monitoring
- Motor valves, mixers, and Frac-920 functionality tested through manual commands
The Amersham Biosciences AKTA Purifier 10 FPLC System is versatile, modular liquid chromatography systems for fast and reliable separations of proteins, peptides, and nucleic acids by FPLC. A KTA Purifier 10 is designed for purification of microgram to milligram quantities of protein, operating at flow rates of 0.001 to 10 ml/min at pressures of 0 to 25 MPa. The Amersham Biosciences AKTA is in excellent condition and has passed all tests for full functionality. These tests included: linking the unit to the Unicorn 5.31 operating system, testing flow rate accuracy of P-900 Pump, testing UPC-900 Monitor with Nal test solution, testing pH/C-900 for functional communication with pH probe and Conductivity Cell, testing motor valves, and testing the Frac-920 for functionality through manual commands. (Additional cost for pH Probe and Holder, please inquire).
The Amersham Biosciences AKTA Purifier 10 FPLC System w/ UPC-900 includes:
- > Box-900
- > P-900 Pump w/ 10ml Pump Heads
- > UPC-900 Monitor
- > INV-907 Valve
- > M-925 Mixer w/ .6ml Mixing Chamber
- > Conductivity Flow Cell
- > CU-950 Communication Module
- > Frac-920 Fraction Collector with Tube Rack
- > Dell Windows 7 Datastation with installed Unicorn version 5.31 software
- > Flat Screen Monitor
- > Installation Calibration Reports
- > Communication Cables
- > Tubing, Power Cords
- > Full 180 Day Parts & Labor Warranty
Amersham Biosciences AKTA Purifier 10 FPLC System Specifications
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P-900
Specifications
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Flow Rate Range
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Isocratic mode: 0.001 to 10 ml/min in steps of 1 ul/min
Gradient mode: 0.001 to 10 ml/min in steps of 1 ul/min
Double mode: 0.001 to 20 ml/min in steps of 1 ul/min
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Pressure Range
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0 to 25 MPa (100 bar, 1450 psi)
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pH Stability Range
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1 to 13 (1 to 14 with <1 day exposure)
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Viscosity
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Max 5 cP
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Flow Rate Accuracy
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± 2% or 20 ul/min, whichever is greater
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Flow Rate Reproducibility
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Flow rate ≥ 0.5 ml/min: rsd < 0.5%
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Gradient Composition
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Accuracy: < ±1% at 0.05 to 10 ml/min
Reproducibility: rsd < 0.25% at 0.5 to 100 ml/min
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Pressure Sensor
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Range: 0 to 27.5 MPa
Offset error: < 0.05 MPa
Scale error: < ±2%
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Total Volume Between Inlet and Outlet
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< 800 ul/module
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UPC
900 Specifications
UV measurement
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Absorbance range
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0.01 - 5 AU (full scale)
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Autozero range
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-0.2 - 2.0 AU
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Baseline adjust Adjustable
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0-100% of full scale
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Wavelengths
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Hg lamp, fixed: 254 and 280
by changing filter:313, 365, 405, 436 and 546 nm
Zn lamp: 214 nm
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Linearity, 5 mm cell
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<3% up to 2 AU at 254 nm
<5% up to 1 AU at 280 nm
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Static noise
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short term: 40x10-6 AU at 254 nm
(typically 6x10-6 AU at 254 nm)
long term: 40x10-6 AU at 254 nm
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Static Drift
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±100x10-6 AU/h at 254 nm
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Flow Sensitivity
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2x10-4 AU min/ml
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M-925
Specifications
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Max Flow Rate
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0.001 to 10 ml/min
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Max Pressure
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12ml chamber: 10 MPa at 0.1 to 100 ml/min (100 bar, 1450 psi)
0.6, 2, and 5ml chambers: 25 MPa at 0.001 to 100 ml/min (250 bar,
3625 psi)
90 ul and 0.2 ml chambers: 35 MPa at 0.001 to 5 ml/min (250 bar, 5075
psi)
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pH Stability Range
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1 to 13 (1 to 14 with <1 day exposure)
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Viscosity
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Max 5 cP
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Internal Volume
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6 interchangeable mixing chambers: 90 ul, 0.2, 0.6, 2, 5, and 12 ml
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Mixing Principle
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1 static chamber and 1 dynamic chamber
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Inlet and Outlet Tubing
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UNF 10 to 32 thread profile
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Frac-920
Specifications
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Capacity
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95 fractions in 10-18 mm tubes
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Depth
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400 mm
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Flow Rate
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< 100 ml/min
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Height
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250 mm
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Operating Temperature
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4° to 40°C
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pH Stability Long Term
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1 – 13
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pH Stability Short Term
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1 – 14
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Program Memory
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99 programs
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Rack Designation
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95 x 10-18 mm (Standard)
175 x 12 mm and 40 x 30 mm (Optional)
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Relative Humidity
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20 to 95% (non condensing)
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Time Mode
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0.01 to 9.999 min
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Volume Mode
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0.1 to 999 ml
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Computer Specifications
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Model
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Dell Optiplex 7010 Desktop PC
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Processor
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Intel Core i5-3470 Processor 3.2GHz
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RAM
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8GB
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Hard Drive
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250GB Internal SSD
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Operating System
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Windows 7 Professional, 64-bit
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- Inspect all displays, replacing any dim or dead pixels to ensure optimal visual feedback
- Examine internal components of P-900, UV-900/UPC-900, and pH/C-900, replacing any malfunctioning parts to maintain performance
- Verify connections of all components via Uni-Net 1 & 2 interfaces for secure and reliable communication
- Check Uni-Net cables, replacing any faulty ones to ensure uninterrupted data transmission
- Inspect internal components of M-925, INV-907, PV-908, and PV-907 to confirm full operational functionality
- Replace all PEEK tubing with new OEM tubing and inspect all components within the flow path for potential clogs
- Confirm successful software interfacing with the FPLC system for seamless operation
- Replace pump piston seals, wash seals, and seal wash tubing, performing ultrasonic cleaning on each check valve in the pump flow path
- Calibrate pressure and flow rates of both the P-900 pump and P-960/950 pump (if applicable) for precise control
- Replace UV lamp and perform internal inspection tests to confirm complete functionality
- Install a new pH probe and verify the conductivity cell for accurate readings
- Calculate and adjust delay volume in software to reflect system configuration
- Clean or replace the drop sensor, accumulator wash, and height adjuster for consistent collection performance
- Verify fraction collector alignment and calibration with 15mL, 50mL, and microplate receptacles to ensure accuracy
- Run installation tests and thoroughly document all findings for comprehensive record-keeping
Technician Inspection and Recertification Checklist: FPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
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Task
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Completed?
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Remarks?
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Inspect all displays and replace dim/dead pixels
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Completed
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Examine internal components of P-900, UV-900/UPC-900, and pH/C-900
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Completed
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Verify connections via Uni-Net 1 & 2 interfaces
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Completed
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Check and replace any malfunctioning Uni-Net cables
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Completed
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Inspect internal components of M-925, INV-907, PV-908, PV-907
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Completed
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Replace PEEK tubing with OEM tubing and check for clogs
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Completed
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Confirm software interfacing with FPLC system
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Completed
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Replace pump seals and wash tubing; clean check valves
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Completed
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Calibrate pressure and flow rates of P-900 and P-960/950 pumps
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Completed
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Replace UV lamp and confirm full functionality
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Completed
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Install new pH probe and verify conductivity cell
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Completed
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Calculate delay volume and update in software
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Completed
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Clean or replace drop sensor, accumulator wash, and height adjuster
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Completed
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Verify fraction collector alignment with 15mL, 50mL, and microplate receptacles
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Completed
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Run installation tests and document all findings
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Completed
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To View More of Our Recertification Protocol Click Here
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