Amersham Biosciences AKTA Purifier 10 FPLC System

P-900 Specifications

Flow Rate Range

Isocratic mode: 0.001 to 10 ml/min in steps of 1 ul/min

Gradient mode: 0.001 to 10 ml/min in steps of 1 ul/min

Double mode: 0.001 to 20 ml/min in steps of 1 ul/min

Pressure Range

0 to 25 MPa (100 bar, 1450 psi)

pH Stability Range

1 to 13 (1 to 14 with <1 day exposure)

Viscosity

Max 5 cP

Flow Rate Accuracy

± 2% or 20 ul/min, whichever is greater

Flow Rate Reproducibility

Flow rate ≥ 0.5 ml/min: rsd < 0.5%

Gradient Composition

Accuracy: < ±1% at 0.05 to 10 ml/min

Reproducibility: rsd < 0.25% at 0.5 to 100 ml/min

Pressure Sensor

Range: 0 to 27.5 MPa

Offset error: < 0.05 MPa

Scale error: < ±2%

Total Volume Between Inlet and Outlet

< 800 ul/module

UPC 900 Specifications
UV measurement

Absorbance range

0.01 - 5 AU (full scale)

Autozero range

-0.2 - 2.0 AU

Baseline adjust Adjustable

0-100% of full scale

Wavelengths

Hg lamp, fixed: 254 and 280

by changing filter:313, 365, 405, 436 and 546 nm

Zn lamp: 214 nm

Linearity, 5 mm cell

<3% up to 2 AU at 254 nm

<5% up to 1 AU at 280 nm

Static noise

short term: 40x10-6 AU at 254 nm

(typically 6x10-6 AU at 254 nm)

long term: 40x10-6 AU at 254 nm

Static Drift

±100x10-6 AU/h at 254 nm

Flow Sensitivity

2x10-4 AU min/ml

M-925 Specifications

Max Flow Rate

0.001 to 10 ml/min

Max Pressure

12ml chamber: 10 MPa at 0.1 to 100 ml/min (100 bar, 1450 psi)

0.6, 2, and 5ml chambers: 25 MPa at 0.001 to 100 ml/min (250 bar, 3625 psi)

90 ul and 0.2 ml chambers: 35 MPa at 0.001 to 5 ml/min (250 bar, 5075 psi)

pH Stability Range

1 to 13 (1 to 14 with <1 day exposure)

Viscosity

Max 5 cP

Internal Volume

6 interchangeable mixing chambers: 90 ul, 0.2, 0.6, 2, 5, and 12 ml

Mixing Principle

1 static chamber and 1 dynamic chamber

Inlet and Outlet Tubing

UNF 10 to 32 thread profile

Frac-920 Specifications

Capacity

95 fractions in 10-18 mm tubes

Depth

400 mm

Flow Rate

< 100 ml/min

Height

250 mm

Operating Temperature

4° to 40°C

pH Stability Long Term

1 – 13

pH Stability Short Term

1 – 14

Program Memory

99 programs

95 x 10-18 mm (Standard)

175 x 12 mm and 40 x 30 mm (Optional)