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Amersham Biosciences AKTA Prime FPLC System
The Amersham Biosciences AKTA Prime is a compact, single-step FPLC system designed for simple purification of proteins. This FPLC system offers an easy-to-use interface with pre-programmed templates, contributing to its high reproducibility and accuracy. The AKTA Prime also features many error-reduction capabilities, such as automatic UV detection and temperature compensation and motorized valves that ensure automatic buffer or sample selection, sample loading, and fraction collection. Fractions and peaks are easily detected via slope sensing within fixed volume collection or automatic peak fractionation. The AKTA Prime is fully compatible with most compact columns, including HiTrap, Hiprep, HiLoad, and RESOURSE, among others.
The Amersham Biosciences AKTA Prime FPLC System is engineered for high quality gel filtration, HIC, affinity chromatography, buffer exchange, and ion exchange. PH Electrode Not Included.
The Amersham Biosciences AKTA Prime FPLC System includes:
- > Dell Laptop with PrimeView Software
- > Conductivity Flow Cell
- > Built in Fraction Collector
- > Power Cords and Cables
- > Operator's Guide
- > Installation Calibration Report
- > Full 180 Day Parts and Labor Warranty
Amersham Biosciences AKTA Prime FPLC System Specifications
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Flow Rate
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0.1ml per minute up to 50ml per minute
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Maximum Operating Pressure (MPa)
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1 MPa or 145 psi
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Wavelength Detection
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254nm and 280nm
Additional filters can be purchased and include
313, 365, 405, 436 and 546nm
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Flow Cell Path Length
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0-14
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Sample Injection
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Up to 2 steps
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pH Monitoring
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Up to 2 samples, this is add-on option
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Viscosity
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10 cp (10ml per minute)
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Realative Humidity
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20% to 95% non-condensing
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Multi Step Purification
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20% to 95% non-condensing
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Tubing I.D.
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0.75 mm for flow path
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Power
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100-120/220-240 VAC 50/60Hz
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Dimensions (W x D x H)
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18” x 14” x 21”
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Weight
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28 lb
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Pharmacia AKTA Prime
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Amersham Biosciences AKTA Prime (This Model)
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GE AKTA Prime Plus
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Generation
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1
st Generation
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2
nd Generation
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3
rd Generation
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Pressure Range
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0–1.0 MPa (10 bar, 145 psi)
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0–1.0 MPa (10 bar, 145 psi)
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0–2.0 MPa (20 bar, 290 psi)
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Flow Rate Accuracy
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±3% or ±0.1 ml/min whichever is greater
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±3% or ±0.1 ml/min whichever is greater
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-5% – +3% of setting (pressure ≥ 0.1 MPa, flow rate ≥ 0.4 ml/min)
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Absorbance Range
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0.01–5.0 AU
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0.01–5.0 AU
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0.01–5.0 AU
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Detector Linearity
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< 3% up to 2 AU at 254 nm < 5% up to 1 AU at 280 nm
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< 3% up to 2 AU at 254 nm < 5% up to 1 AU at 280 nm
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< 3% up to 2 AU at 254 nm < 5% up to 1 AU at 280 nm
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Static Noise
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40x10-6 AU at 254 nm
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40x10-6 AU at 254 nm
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40x10-6 AU at 254 nm
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Wavelengths
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254nm and 280nm (optional filters include: 313, 365, 405, 436 and 546 nm)
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254nm and 280nm (optional filters include: 313, 365, 405, 436 and 546 nm)
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254nm and 280nm (optional filters include: 313, 365, 405, 436 and 546 nm)
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AKTA Prime Operator's Manual
 Click Here to View all the Technical Specs for the AKTA Prime FPLC System
- Inspect all displays, replacing any dim or dead pixels to ensure optimal visual feedback
- Examine internal components of P-900, UV-900/UPC-900, and pH/C-900, replacing any malfunctioning parts to maintain performance
- Verify connections of all components via Uni-Net 1 & 2 interfaces for secure and reliable communication
- Check Uni-Net cables, replacing any faulty ones to ensure uninterrupted data transmission
- Inspect internal components of M-925, INV-907, PV-908, and PV-907 to confirm full operational functionality
- Replace all PEEK tubing with new OEM tubing and inspect all components within the flow path for potential clogs
- Confirm successful software interfacing with the FPLC system for seamless operation
- Replace pump piston seals, wash seals, and seal wash tubing, performing ultrasonic cleaning on each check valve in the pump flow path
- Calibrate pressure and flow rates of both the P-900 pump and P-960/950 pump (if applicable) for precise control
- Replace UV lamp and perform internal inspection tests to confirm complete functionality
- Install a new pH probe and verify the conductivity cell for accurate readings
- Calculate and adjust delay volume in software to reflect system configuration
- Clean or replace the drop sensor, accumulator wash, and height adjuster for consistent collection performance
- Verify fraction collector alignment and calibration with 15mL, 50mL, and microplate receptacles to ensure accuracy
- Run installation tests and thoroughly document all findings for comprehensive record-keeping
Technician Inspection and Recertification Checklist: FPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
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Task
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Completed?
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Remarks?
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Inspect all displays and replace dim/dead pixels
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Completed
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Examine internal components of P-900, UV-900/UPC-900, and pH/C-900
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Completed
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Verify connections via Uni-Net 1 & 2 interfaces
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Completed
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Check and replace any malfunctioning Uni-Net cables
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Completed
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Inspect internal components of M-925, INV-907, PV-908, PV-907
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Completed
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Replace PEEK tubing with OEM tubing and check for clogs
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Completed
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Confirm software interfacing with FPLC system
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Completed
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Replace pump seals and wash tubing; clean check valves
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Completed
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Calibrate pressure and flow rates of P-900 and P-960/950 pumps
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Completed
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Replace UV lamp and confirm full functionality
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Completed
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Install new pH probe and verify conductivity cell
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Completed
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Calculate delay volume and update in software
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Completed
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Clean or replace drop sensor, accumulator wash, and height adjuster
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Completed
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Verify fraction collector alignment with 15mL, 50mL, and microplate receptacles
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Completed
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Run installation tests and document all findings
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Completed
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To View More of Our Recertification Protocol Click Here
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