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Amersham Biosciences AKTA FPLC Explorer 100 System
The Amersham Biosciences AKTA Explorer 100 FPLC System is in excellent condition and has passed all tests for full functionality. These tests included: linking the unit to the Unicorn 5.31 operating system, testing flow rate accuracy of P-900 Pump, testing operation of pressure sensor in P-900 and P-950, leak-testing P-900 and P-950, testing UV-900 Monitor with Nal test solution, testing pH/C-900 for functional communication with pH probe and Conductivity Cell, testing functionality of the 925 Sensor, testing motor valves, and testing the Frac-950 for functionality through manual commands. Configured setup for detection is UV Absorbance, pH, and Conductivity. Fraction collection is through the Frac-950.
The Amersham Biosciences AKTA FPLC Explorer 100 System includes:
- > Box-900
- > P-900 Pump w/Switch Valves
- > UV-900 Detector
- > UV Flow Cell
- > UV Lamp
- > pH/C-900 Monitor
- > P-960 Sample Pump
- > M-925 Mixer
- > 2mL Mixing Chamber M-925
- > Conductivity Cell
- > pH Probe and Holder
- > CU-950 Control Box
- > Injection V1 INV-907
- > Column V2 PV-908
- > Column V3 PV-908
- > Outlet V4 PV-908
- > Sample V5 Valve PV-908
- > Buffer Valve V6 IV-908
- > Flow Direction Valve V7 INV-907
- > FR-902 Flow Restrictor
- > Inline Filter and Tubing
- > Frac-950 Fraction Collector & Rack
- > Dell Datastation Windows 7 Pro with Unicorn version 5.31 Software
- > Flat Screen Monitor
- > Installation Calibration Report
- > Communication and Power Cables
- > Full 180 Day Parts and Labor Warranty
Amersham Biosciences AKTA FPLC Explorer 100 System Specifications
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UV-900 Detector Specifications
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Wavelength
range
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190–700 nm in
steps of 1 nm,
3 wavelengths
simultaneously
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Bandwidth
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4 nm
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Wavelength
accuracy
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±2 nm
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Wavelength
reproducibility
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< 500 ms
(one cycle from 214 nm to
254 nm and
back to 214 nm)
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Linearity
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< 2%
deviation up to 2 AU at 260 nm with
Uracil at pH
2
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Static noise
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short term:
< 6x10-5 AU at 230 nm
long term:
< 6x10-5 AU at 230 nm
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Drift
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< 2x10-4
AU/h at 254 nm
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Flow Cell
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Optical path
length 2 mm cell
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2 mm, 2 µl
cell volume
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Optical path
length 10 mm cell
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10 mm, 8 µl
cell volume
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Max. flow
rate
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100 ml/min
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Max. pressure
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2 MPa (290
psi)
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Temperature
range
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+4 to +40 °C
(liquid)
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Wetted
material
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PTFE
(polytetrafluoroethylene)
PEEK
(polyetheretherketone)
titanium
(palladium alloy),
quartz
(synthetic fused silica)
and titanium
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P-900 Pump Specifications
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Flow Rate
Range
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Isocratic
mode: 0.01 to 100 ml/min in steps of 10 ul/min
Gradient
mode: 0.01 to 100 ml/min in steps of 10 ul/min
Double mode:
0.01 to 200 ml/min in steps of 10 ul/min
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Pressure
Range
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0 to 10 MPa
(100 bar, 1450 psi)
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pH Stability
Range
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1 to 13 (1 to
14 with <1 day exposure)
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Viscosity
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Max 5 cP
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Flow Rate
Accuracy
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± 2% or 20
ul/min, whichever is greater
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Flow Rate
Reproducibility
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Flow
rate ≥ 0.5 ml/min: rsd < 0.5%
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Gradient
Composition
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Accuracy:
< ±1% at 0.05 to 10 ml/min
Reproducibility:
rsd < 0.25% at 0.5 to 100 ml/min
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Pressure
Sensor
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Range: 0 to
27.5 MPa
Offset error:
< 0.05 MPa
Scale error:
< ±2%
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Total Volume
Between Inlet and Outlet
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M-925 Mixer Specifications
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Max Flow Rate
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0.001 to 100
ml/min
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Max Pressure
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12ml chamber:
10 MPa at 0.1 to 100 ml/min (100 bar, 1450 psi)
0.6, 2, and
5ml chambers: 25 MPa at 0.001 to 100 ml/min (250 bar, 3625 psi)
90 ul and 0.2
ml chambers: 35 MPa at 0.001 to 5 ml/min (250 bar, 5075 psi)
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pH Stability
Range
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1 to 13 (1 to
14 with <1 day exposure)
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Viscosity
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Max 5 cP
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Internal
Volume
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6
interchangeable mixing chambers: 90 ul, 0.2, 0.6, 2, 5, and 12 ml
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Mixing
Principle
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1 static
chamber and 1 dynamic chamber
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Inlet and
Outlet Tubing
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UNF 10 to 32
thread profile
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Frac-950 Fraction Collector Specifications
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Max Flow Rate
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100 ml/min
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pH Stability
Range
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1 to 13 (1 to
14 with <1 day exposure)
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Fraction Size
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0.1 to
99999.99 ml (volume mode)
0.1 to
99999.99 min (time mode)
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Standard Mode
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u-liter
plates, DropSync: 0 to 1 ml/min
12 mm rack,
DropSync: 0 to 1.5 ml/min
18 and 30 mm
racks, DropSync: 0 to 2 ml/min
18 and 30 mm racks, Accumulator: 15 to 100ml/min
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Prep Mode
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30 mm rack, Accumulator: 15 to 100 ml/min
Funnel-to-flask, Accumulator: 15 to 100 ml/min
250ml bottles, Accumulator: 15 to 100 ml/min
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Environmental Conditions
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+4° to +40°C
20 to 95% relative humidity
84 to 106 kPa (840 to 1060 mbar) atmospheric pressure
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- Inspect all displays, replacing any dim or dead pixels to ensure optimal visual feedback
- Examine internal components of P-900, UV-900/UPC-900, and pH/C-900, replacing any malfunctioning parts to maintain performance
- Verify connections of all components via Uni-Net 1 & 2 interfaces for secure and reliable communication
- Check Uni-Net cables, replacing any faulty ones to ensure uninterrupted data transmission
- Inspect internal components of M-925, INV-907, PV-908, and PV-907 to confirm full operational functionality
- Replace all PEEK tubing with new OEM tubing and inspect all components within the flow path for potential clogs
- Confirm successful software interfacing with the FPLC system for seamless operation
- Replace pump piston seals, wash seals, and seal wash tubing, performing ultrasonic cleaning on each check valve in the pump flow path
- Calibrate pressure and flow rates of both the P-900 pump and P-960/950 pump (if applicable) for precise control
- Replace UV lamp and perform internal inspection tests to confirm complete functionality
- Install a new pH probe and verify the conductivity cell for accurate readings
- Calculate and adjust delay volume in software to reflect system configuration
- Clean or replace the drop sensor, accumulator wash, and height adjuster for consistent collection performance
- Verify fraction collector alignment and calibration with 15mL, 50mL, and microplate receptacles to ensure accuracy
- Run installation tests and thoroughly document all findings for comprehensive record-keeping
Technician Inspection and Recertification Checklist: FPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
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Task
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Completed?
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Remarks?
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Inspect all displays and replace dim/dead pixels
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Completed
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Examine internal components of P-900, UV-900/UPC-900, and pH/C-900
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Completed
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Verify connections via Uni-Net 1 & 2 interfaces
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Completed
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Check and replace any malfunctioning Uni-Net cables
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Completed
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Inspect internal components of M-925, INV-907, PV-908, PV-907
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Completed
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Replace PEEK tubing with OEM tubing and check for clogs
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Completed
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Confirm software interfacing with FPLC system
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Completed
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Replace pump seals and wash tubing; clean check valves
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Completed
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Calibrate pressure and flow rates of P-900 and P-960/950 pumps
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Completed
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Replace UV lamp and confirm full functionality
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Completed
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Install new pH probe and verify conductivity cell
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Completed
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Calculate delay volume and update in software
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Completed
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Clean or replace drop sensor, accumulator wash, and height adjuster
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Completed
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Verify fraction collector alignment with 15mL, 50mL, and microplate receptacles
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Completed
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Run installation tests and document all findings
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Completed
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To View More of Our Recertification Protocol Click Here
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good fplc 
