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Agilent G6230A TOF-Mass Spectrometer JetStream ESI Source w/ 1260 UPLC System
The Agilent G6230A Time-of-Flight Mass Spectrometer system provides accurate mass measurement with high mass resolution for confident compound identification beyond HPLC detection. The Agilent TOF is an orthogonal acceleration time-of-flight mass spectrometer (oa-TOF). The ions reaching the time-of-flight chamber are impelled in a direction perpendicular to their original path, meaning that the acceleration pulse applied to send the ions down the flight tube is orthogonal to the direction that ions are entering the mass analyzer. This geometry minimizes the effect of the entrance velocity on the flight time, leading to higher resolution. This TOF-MS system provides full-spectrum data for screening, profiling, and identification of small molecules, large biologic compounds, and everything in between making the 6230 ideal for applications like drug development, toxicology, and the analysis of intact proteins.
The Agilent G6230A Time-of-Flight Mass Spectrometer can be set up with the Agilent 1200, 1260, 1260 Infinity II, 1290 Infinity, or 1290 Infinity II UPLC modules and with one of serval ion interfaces. This specific configuration for the G2630A TOF-MS adds on the Agilent 1260 UPLC module, along with utilizing the Agilent JetStream ESI ion source. The AJS ESI source uses a super-heated sheath gas to collimate the nebulizer spray which dramatically increases the number of ions that enter the mass spectrometer. The Agilent G6230A TOF-MS also allows for the user to choose between 3 unique acquisition modes: 1GHz Extended Mass Range Mode: which allows for an extended mass range up to 20,000 m/z, 2Ghz Extended Dynamic Range Mode: which features up to 5 orders of magnitude and a scan range of 25 to 3,200 m/z, and 4Ghz High Resolution Mode: which features high resolution scanning at 2,721 m/z.
The Agilent G6230A TOF-MS can be controlled from the Agilent MassHunter Workstation Software, which allows for programs to run in data acquisition, qualitative analysis, quantitative analysis, and the added-on BioConfirm, for an all-in-one software bundle.
This Agilent system is in excellent condition and will be fully tested to perform at factory
specifications before being shipped.
The Agilent G6230A TOF-Mass Spectrometer JetStream ESI Source w/ 1260 UPLC System includes:
- > Edwards E2M28 Rotary Vane Vacuum Pump
- > K4225A Vacuum Degasser
- > G1312B Binary Pump
- > G1367D HiP Wellplate Sampler
- > G1330B ALS Thermostat
- > G1316B SL Column Compartment
- > Windows 10 Professional 64-Bit Data Station pre-loaded with MassHunter Software including Acquisition, Qual, Quant, and BioConfirm SW
- > All Power Cords
- > Full 180 Day Parts and Labor Warranty
Below is a List of Diagnostic Tests Performed On This System
- Pressure Test: The pressure test is a quick, built-in test designed to demonstrate the pressure tightness (up to 400 bar) of the system.
- Leak Test: The leak test is a built-in troubleshooting designed to demonstrate the leak-tightness of the pump.
- Thermostat Test: Evaluates the cooling and heating performance of the two Peltier elements.
- Injector Steps: Performs each movement of the sampling sequence under manual control. This is useful for confirming autosampler functionality.
- 4 Peak Test (Isocratic Standard): Standard test published by Agilent to confirm complete functionality of the 1260 UPLC System.
Agilent G2930A TOF-Mass Spectrometer JetStream ESI Source Specifications
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Parameter
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Specification
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MS Sensitivity with ESI
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Signal-to-noise ratio > 50:1
Noise = 1 x RMS
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Mass Resolving Power
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22,000 FWHM
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Mass Accuracy - MS Mode
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< 1 ppm
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Spectral Acquisition Rate - MS Mode
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30 spectra/second
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Dynamic Range
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Up to 5 decades
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Mass Range
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25 to 20,000 m/z
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Polarity Switching
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< 1 second
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Mass Accuracy Temperature Stability - MS Mode
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Maintain 2 ppm mass accuracy within 2°C drift per hour
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Single Point of Control
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Single point data system method capability with full control for LC and TOF systems
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Dimensions (L x W x H)
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32.7 x 28.7 x 52.4 in (83 x 73 x 133 cm)
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Weight
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260 lbs. (117.9 kg)
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Click Here to View Specifications for The Agilent 1260 System
Agilent 1260 UPLC System Specifications
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Vacuum Degasser
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Number of Solvent Channels
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4
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Flow Range
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0 - 10 ml/min per channel
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Internal Volume per Channel
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0.45 ml per channel
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pH Range
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1 - 14
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Binary Pump
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Settable Flow Range
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Set points 0.001 - 5 ml/min, in 0.001 ml/min increments
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Flow Range
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0.05 - 5.0 ml/min
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Flow Precision
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≤ 0.07% RSD or ≤ 0.02 min SD
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Flow Accuracy
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± 1% or 10 µl/min
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Pressure Operating Range
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0 - 60 MPa (0 - 600 bar, 0 - 8,700 psi) up to 5 ml/min
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Pressure Pulsation
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< 2% amplitude or < 0.3 MPa (3 bar) at 1 ml/min isopropanol, at all pressures > 1 MPa (10 bar, 147 psi)
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Composition Range
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Settable range: 0 - 100%
Recommended range: 1 - 99% or 5 µl/min per channel
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Composition Precision
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< 0.15 % RSD or < 0.04 min SD
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Composition Accuracy
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± 0.35% absolute, at 2 ml/min, at 10 MPa (100 bar)
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HiP Wellplate Sampler
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Injection Range
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0.1 - 40 µl in 0.1 µl increments
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Precision
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Typically < 0.25% RSD from 5 - 40 µl, typically < 0.5% RSD from 2 - 5 µl, < 0.7% RSD from 1 - 2 µl
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Pressure Range
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Up to 600 bar (8,700 psi)
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Sample Viscosity Range
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0.2 - 5 cp
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Sample Capacity
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2 × well plates (MTP) + 10 × 2 ml vials 108 x 2-mL vials in2 x 54 vial
plate plus 10 additional 2 mL vials 30 x 6-mL vials in 2 x 15 vial plate
plus 10 additional 2 mL vials
54 Eppendorf tubes (0.5/1.5/2.0mL) in 2 x 27 Eppendorf tube plate
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Injection Cycle Time
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Typically < 17 seconds
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ALS Thermostat
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Temperature Range
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4°C to 40°C in 1°C increments
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Temperature Accuracy at Ambient Temperatures
< 25°C and Humidity < 50%
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-1°C to +4°C at a setpoint of 4°C
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Temperature Accuracy at Ambient Temperatures
> 25°C and/or Humidity > 50%
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-1°C to +5°C at a setpoint of 4°C
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SL Column Compartment
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Temperature Range
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Ambient -10°C to 80°C at flow rates up to 5 ml/min
Ambient -10°C to 100°C at flow rates up to 2.5 ml/min
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Temperature Stability
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± 0.05°C
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Temperature Accuracy
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± 0.8°C
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Warm-Up/Cool-Down Time
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5 minutes from ambient to 40°C
10 minutes from 40°C to 20°C
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Computer Specifications
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Model
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Dell Optiplex 7010 Desktop PC
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Processor
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Intel Core i5-3470 Processor 3.2GHz
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RAM
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16GB
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Hard Drive
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250GB Internal SSD
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Operating System
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Windows 10 Professional, 64bit
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