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Agilent BioTek Synergy HTX Multi-Mode Microplate Reader
Agilent BioTek Synergy HTX Multi-Mode Microplate Reader
 
 



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Condition: Fully Recertified and Tested to Perform at Manufacturer Specifications
Warranty: 180-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 1 to 2 Business Days
Included Documentation: ABS, FLD, LUM, and Self Test Calibration Report

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Description
Recertification Protocol
Technical Specification Sheet
 

Agilent BioTek Synergy HTX Multi-Mode Microplate Reader



Detection Modes
Absorbance, Fluorescence Intensity, Luminescence

Microplate Formats
6-,12-,24-,48-,96-, 384-wells

Read Types
Endpoint, Kinetic, Spectral Scanning, Well-Area Scanning

Features
  • Features UV-Vis absorbance, filter-based fluorescence, and luminescence, suitable for a wide range of end point and kinetic applications
  • Equipped with a xenon flash lamp and monochromator for filter-free wavelength selection (200 to 999 nm) in absorbance measurements and a tungsten halogen lamp with interference filters for high-sensitivity fluorescence detection
  • Includes Agilent BioTek’s unique 4-Zone incubation system, capable of maintaining temperatures up to 50°C, perfect for various assay requirements
  • Accommodates a broad range of microplate formats, from 6 to 384 wells, providing flexibility for different experimental scales
  • Controlled by the powerful Agilent BioTek Gen5 software, facilitating easy data collection, analysis, exporting, and reporting
  • Compatible with the BioTek BioStack microplate stacker for automated processing of up to 50 microplates, enhancing workflow efficiency
  • Suitable for nucleic acid and protein quantification, enzyme kinetics, ELISAs, genetic analysis, cell proliferation, cytotoxicity, and more
  • Features cell-friendly orbital and linear shaking
The Agilent BioTek Synergy HTX Multi-Mode Microplate Reader is a versatile and high-performing instrument designed for a variety of laboratory applications. It features UV-Vis absorbance, filter-based fluorescence, and luminescence capabilities, making it suitable for a variety of different applications. The reader’s unique dual-optics design includes a xenon flash lamp and monochromator for absorbance measurements across a wide wavelength range (200 to 999 nm) and a tungsten halogen lamp with interference filters for sensitive fluorescence detection. This compact system is further enhanced with the Agilent BioTek's unique 4-Zone incubation up to 50°C, the ability to add-on dual reagent injectors, and options for linear and orbital shaking, accommodating 6- to 384-well microplates. The Synergy HTX is compatible with the user-friendly Agilent BioTek Gen5 software, streamlining data collection, analysis, exporting, and reporting.

Additionally, the Synergy HTX is designed for integration with the BioTek BioStack microplate stacker for increased automation and throughput, capable of processing up to 50 microplates simultaneously. The reader is ideal for a range of applications, including nucleic acid and protein quantification, enzyme kinetics, biomarker quantification, ELISAs, genetic analysis, cell proliferation, cytotoxicity, drug absorption and metabolism, food safety, and environmental monitoring. It's also equipped for low volume (2 μL) nucleic acid quantification with Take3 and Take3 Trio plates, and features an advanced incubator design with Condensation Control to minimize plate lid condensation. The device is alpha assay capable, modular, upgradable, and compatible with third-party automation solutions, offering convenience, versatility, and affordability for various scientific research needs.

All of our equipment is fully tested to perform at factory specifications before being shipped.

INCLUDED:
The Agilent BioTek Synergy HTX Multi-Mode Microplate Reader includes:
  • > Windows 11 Professional 64-Bit Data Station with Pre-Loaded Gen5 PC Software
  • > 460 nm, 528 nm, 590 nm, and 645 nm Emission Filters
  • > 360 nm, 485 nm, 530 nm, and 590 nm Excitation Filters
  • > ABS, FLD, LUM, and Self Test Calibration Report
  • > Interface Cable
  • > Operator's Manual
  • > Full 180 Day Part and Labor Warranty

Agilent BioTek Synergy HTX Multi-Mode Microplate Reader Specifications

Absorbance Specifications

Light Source

Xenon flash lamp

Detector

Photodiode

Wavelength Selection

Monochromator

Wavelength Range

200 to 999 nm, in 1 nm increments

Monochromator Bandwidth

2.4 nm

Monochromator Wavelength Accuracy

± 2 nm

Monochromator Wavelength Repeatability

± 0.2 nm

Dynamic Range

0 to 4.0 OD

Photometric Resolution

0.0001 OD

Pathlength Correction

Yes

Photometric Linearity

< 1% from 0 to 3.0 OD

Photometric Repeatability

< 0.5% at 2.0 OD

Fluorescence Intensity Specifications

Light Source

Tungsten halogen

Detector

PMT

Wavelength Selection

Filter-based

Wavelength Range

300 to 700 nm

Dynamic Range

> 6 decades

Sensitivity (Top and Bottom)

Fluorescein 5 pM (1 fmol/well, 96-well plate)

Luminescence Specifications

Dynamic Range

< 6 decades

Sensitivity

30 amol ATP (flash)

Detection System

Low noise PMT

Wavelength Range

300 to 700 nm

Alpha Detection Specifications

Light Source

Tungsten halogen

Sensitivity

300 amol of biotinylated LCK-P peptide

Read Speed

2 minutes (96-well plate)

General Specifications

Microplate Formats

6, 12, 24, 48, 96, 384 wells

Read Methods

End point, kinetic, spectral scanning, well-area scanning

Plate Shaking

Linear and orbital

Temperature Control

4-Zone incubation to 50°C, ± 0.2°C at 37°C

Dimensions (H x W x D)

12 x 15 x 15 in. (30.5 x 38.1 x 38.1 cm)

Weight

27 lb. (12.3 kg.)

Electrical Requirements

100-240V, 50/60Hz



Computer Specifications

Processor Intel Core I5-8350U CPU
RAM 16.0 GB
Hard Drive 512GB Internal SSD
Operating System Windows 11 Professional, 64-bit
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