Agilent 6230B Accurate-Mass TOF Mass Spectrometer

The Agilent 6230B Accurate-Mass TOF Mass Spectrometer is a high-performance time-of-flight (TOF) system designed to provide precise, high-resolution mass measurements for a wide range of applications, including drug discovery, metabolomics, and environmental analysis. Utilizing orthogonal acceleration (oa-TOF) technology, this instrument directs ions perpendicularly to their original trajectory before they enter the flight tube, reducing entrance velocity effects and enhancing resolution. With its ability to capture a full mass spectrum scan up to 20,000 m/z, the 6230B delivers superior analytical capabilities, allowing for accurate identification of unknown compounds, advanced statistical analysis, and in-depth biomolecule characterization. Equipped with Agilent Jet Stream (AJS) electrospray ionization (ESI) technology, the system enhances ion generation and transmission efficiency through a super-heated sheath gas, significantly increasing sensitivity while maintaining low detection limits. The 6230B ensures high-throughput sample screening with superior mass accuracy, reducing false positives with a precision of better than 1 ppm. This precision is maintained even in demanding workflows, supported by a high spectral acquisition rate of up to 30 spectra per second, enabling robust performance for fast ultra-high-performance liquid chromatography (UHPLC) separations.

The Agilent 6230B also offers advanced molecular profiling tools through the intuitive MassHunter software suite, featuring Find by Formula for compound identification based on accurate mass, isotopic abundance, and chemical composition. Additionally, the system supports high-resolution separation of complex mixtures, enabling confident elemental formula determination. With a broad dynamic range of up to five orders of magnitude, this TOF mass spectrometer can detect trace-level analytes even in complex matrices, making it a powerful tool for impurity profiling and advanced biological analysis. Designed for versatility, the 6230B supports multiple ionization sources, including ESI, atmospheric pressure chemical ionization (APCI), atmospheric pressure photoionization (APPI), multimode (ESI and APCI), HPLC-Chip/MS interface, and AP/MALDI PDF+ sources. It incorporates a dual-sprayer orthogonal ESI source with a built-in calibrant delivery system, allowing for real-time internal reference mass correction. The system's single-point data control integrates HPLC and TOF operations, automatically stopping sample injections if errors or leaks occur, improving workflow efficiency and reducing operational risks.

With a resolving power of 22,000 full width at half maximum (FWHM) and a mass accuracy stability of 2 ppm even with temperature fluctuations, the Agilent 6230B provides consistently high-quality data. The system’s automated tuning and calibration capabilities optimize ion optics and TOF MS mass axis alignment, ensuring peak performance with minimal user intervention. For laboratories requiring untargeted screening, the system offers optional in-source dissociation for fragmentation data, further expanding its analytical capabilities. Built to streamline laboratory operations, the Agilent 6230B features MassHunter software, simplifying workflows and minimizing administrative tasks while enabling efficient, expert-level analysis without the need for extensive LC/MS expertise. Whether analyzing monoclonal antibodies, glycoforms, truncations, or other biologic compounds, the Agilent 6230B provides unparalleled accuracy, resolution, and sensitivity, making it an essential instrument for laboratories seeking cutting-edge mass spectrometry performance.

This Agilent MS system was recertified and calibrated by a factory trained engineer and is ready to ship on demand. This system comes with everything needed for easy installation and setup. All of our equipment is fully tested to perform at factory specifications before being shipped.

Agilent 6230B Accurate-Mass TOF Mass Spectrometer Specifications

MS Sensitivity with AJS (Included)	Signal-to-noise ratio > 50:1, Noise = 1 × RMS (1 pg reserpine injected on-column, measured at m/z 609.2807)
MS Sensitivity with ESI (Option 200)	Signal-to-noise ratio > 10:1, Noise = 1 × RMS (1 pg reserpine injected on-column, measured at m/z 609.2807)
Mass Resolving Power	22,000 FWHM (measured at m/z 1,522 after automatic tuning procedure)
Mass Accuracy - MS Mode	< 1 ppm (measured at m/z 609.2807 using an internal reference mass)
Spectral Acquisition Rate - MS Mode	30 spectra/second (spectra acquisition from m/z 100 to 3,200)
Dynamic Range	Up to 5 decades (in-scan dynamic range on co-eluting components)
Mass Range	m/z 25 to 20,000
Polarity Switching	< 1 sec (pos/neg switching)
Mass Accuracy Temperature Stability - MS Mode	Maintain 2 ppm mass accuracy within 2°C drift per hour (temperature: 15°C to 35°C (59°F to 95°F) at constant temperature while setting autotune at 25°C)
Single Point of Control	Single point data system method capability with full control for HPLC system and TOF, stopping sample injection if errors or leaks occur
Ionization sources	Electrospray (ESI), Atmospheric pressure chemical ionization (APCI), Atmospheric pressure photoionization (APPI), Multimode source (ESI and APCI), HPLC-Chip/MS interface, AP/MALDI PDF+ source
Transparent Introduction of Reference Masses	Dual-sprayer orthogonal ESI source, built-in calibrant delivery system, real-time internal reference mass correction for MS operation and heated sources
Autotune	Automated optimization of ion optics and full TOF MS mass axis calibration
Data Mining Tools	One-button extraction of compound-specific spectral and chromatographic information using find compounds algorithms
Molecular Profiling Tools	Measurement of molecular features from LC/MS analyses (MassHunter), differential expression analysis with optional MassHunter Mass Profiler
Sound Emission	< 70 db
Dimensions (L x W x H)	32.7 x 28.7 x 52.4 in. (83 x 73 x 133 cm)
Weight	260 lb. (117.9 kg)
Electrical Requirements	200-220VAC, 50/60Hz, 15A

Computer Specifications
Model	Dell Optiplex 7010 Desktop PC
Processor	Intel Core i5-6500 Processor 3.2GHz
RAM	16GB
Hard Drive	250GB Internal SSD
Operating System	Windows 10 Professional, 64bit

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