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Our Price: $109,995.00

Condition: Fully Recertified and Tested to Perform at Manufacturer Specifications
Warranty: 180-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 2 to 3 Weeks
Included Documentation Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report & Mass Spec Tuning Report

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Product Code: AG-6230BLCMS1260

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Description

Recertification Protocol

Technical Specification Sheet

Agilent 6230B Accurate-Mass TOF Mass Spectrometer w/ 1260 Infinity I LC System

Mass Range
m/z 25 to 20,000

Mass Accuracy
< 1 ppm (measured at m/z 609.2807)

Spectral Acquisition Rate
30 spectra/second (from m/z 100 to 3,200)

Features

Orthogonal acceleration TOF design for enhanced resolution and minimized entrance velocity effects
Agilent Jet Stream ESI technology for increased ionization efficiency and low picogram sensitivity
Dynamic range spanning five orders of magnitude for detecting trace-level compounds in complex matrices
Automated tuning and calibration for consistent performance and reduced user intervention
Real-time internal reference mass correction with built-in calibrant delivery system
Resolving power of 22,000 FWHM for separating closely related compounds
Polarity switching in under 1 second for versatile positive and negative ion analysis
Single-point data control for seamless integration with HPLC systems and error management
Optional in-source dissociation for untargeted screening and enhanced fragmentation data

The Agilent 6230B Accurate-Mass TOF Mass Spectrometer w/ 1260 Infinity I LC System is a high-performance time-of-flight (TOF) system designed to provide precise, high-resolution mass measurements for a wide range of applications, including drug discovery, metabolomics, and environmental analysis. Utilizing orthogonal acceleration (oa-TOF) technology, this instrument directs ions perpendicularly to their original trajectory before they enter the flight tube, reducing entrance velocity effects and enhancing resolution. With its ability to capture a full mass spectrum scan up to 20,000 m/z, the 6230B delivers superior analytical capabilities, allowing for accurate identification of unknown compounds, advanced statistical analysis, and in-depth biomolecule characterization. Equipped with Agilent Jet Stream (AJS) electrospray ionization (ESI) technology, the system enhances ion generation and transmission efficiency through a super-heated sheath gas, significantly increasing sensitivity while maintaining low detection limits. The 6230B ensures high-throughput sample screening with superior mass accuracy, reducing false positives with a precision of better than 1 ppm. This precision is maintained even in demanding workflows, supported by a high spectral acquisition rate of up to 30 spectra per second, enabling robust performance for fast ultra-high-performance liquid chromatography (UHPLC) separations.

The Agilent 6230B also offers advanced molecular profiling tools through the intuitive MassHunter software suite, featuring Find by Formula for compound identification based on accurate mass, isotopic abundance, and chemical composition. Additionally, the system supports high-resolution separation of complex mixtures, enabling confident elemental formula determination. With a broad dynamic range of up to five orders of magnitude, this TOF mass spectrometer can detect trace-level analytes even in complex matrices, making it a powerful tool for impurity profiling and advanced biological analysis. Designed for versatility, the 6230B supports multiple ionization sources, including ESI, atmospheric pressure chemical ionization (APCI), atmospheric pressure photoionization (APPI), multimode (ESI and APCI), HPLC-Chip/MS interface, and AP/MALDI PDF+ sources. It incorporates a dual-sprayer orthogonal ESI source with a built-in calibrant delivery system, allowing for real-time internal reference mass correction. The system's single-point data control integrates HPLC and TOF operations, automatically stopping sample injections if errors or leaks occur, improving workflow efficiency and reducing operational risks.

With a resolving power of 22,000 full width at half maximum (FWHM) and a mass accuracy stability of 2 ppm even with temperature fluctuations, the Agilent 6230B provides consistently high-quality data. The system’s automated tuning and calibration capabilities optimize ion optics and TOF MS mass axis alignment, ensuring peak performance with minimal user intervention. For laboratories requiring untargeted screening, the system offers optional in-source dissociation for fragmentation data, further expanding its analytical capabilities. Built to streamline laboratory operations, the Agilent 6230B features MassHunter software, simplifying workflows and minimizing administrative tasks while enabling efficient, expert-level analysis without the need for extensive LC/MS expertise. Whether analyzing monoclonal antibodies, glycoforms, truncations, or other biologic compounds, the Agilent 6230B provides unparalleled accuracy, resolution, and sensitivity, making it an essential instrument for laboratories seeking cutting-edge mass spectrometry performance.

This Agilent MS system was recertified and calibrated by a factory trained engineer and is ready to ship on demand. This system comes with everything needed for easy installation and setup. All of our equipment is fully tested to perform at factory specifications before being shipped.

INCLUDED:
The Agilent 6230B Accurate-Mass TOF Mass Spectrometer w/ 1260 Infinity I LC System includes:

> Agilent 6130B Single Quadrupole Mass Spec
> Agilent MS40+ Rotary Vane Vacuum Pump
> Agilent G1958B Jet Stream Electrospray Ionization Source
> G1312B Binary Pump (G1311B Quaternary Pump available, please inquire)
> G1329B Autosampler
> G1330B ALS Thermostat
> G1316A TCC
> G1379B Degasser
> G4212B DAD Detector
> Windows 10 Professional 64-Bit Data Station with Flatscreen Monitor
> Agilent MassHunter B.09.01 Acquisition and Data Analysis Software Installed
> Agilent MassHunter BioConfirm Software Installed
> All Power Cords
> Can/Crossover Cables
> Agilent Lan Interface Card Built-In
> Solvent Tubing
> Capillaries
> Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report & Mass Spec Tuning Report
> Full 180 Day Parts and Labor Warranty

Below is a List of Diagnostic Tests Performed On This System
G4212B Diode Array Detector Diagnostic Tests:

Filter Test: The test moves the filter into the light path and measures the relative absorbance in the filter position.
Dark Current Test: The test measures the dark current from the detector optic.
Holmium Oxide Test: The test measures the Holmium spectrum from the built-in Holmium filter. The spectrum is evaluated for peaks at different wavelengths.
Lamp Intensity Test: The test verifies functionality of the UV lamp at various wavelengths.
Wavelength Intensity Test: The test confirms the accuracy of wavelengths measured.
Baseline Noise Test: The test measures the amount of baseline noise drift identified by the detector.

G1312B Binary Pump Diagnostic Tests:

Pressure Test: The pressure test is a quick, built-in test designed to demonstrate the pressure tightness (up to 400 bar) of the system.
Leak Test: The leak test is a built-in troubleshooting designed to demonstrate the leak-tightness of the pump.

G1316A Column Compartment Diagnostic Tests:

Thermostat Test: Evaluates the cooling and heating performance of the two Peltier elements.

G1329B Autosampler Diagnostic Tests:

Injector Steps: Performs each movement of the sampling sequence under manual control. This is useful for confirming autosampler functionality.

System Tests:

4 Peak Test (Isocratic Standard): Standard test published by Agilent to confirm complete functionality of the 1200 HPLC System.

Agilent 6230B Accurate-Mass TOF Mass Spectrometer w/ 1260 Infinity I LC System Specifications

MS Sensitivity with AJS (Included)	Signal-to-noise ratio > 50:1, Noise = 1 × RMS (1 pg reserpine injected on-column, measured at m/z 609.2807)
MS Sensitivity with ESI (Option 200)	Signal-to-noise ratio > 10:1, Noise = 1 × RMS (1 pg reserpine injected on-column, measured at m/z 609.2807)
Mass Resolving Power	22,000 FWHM (measured at m/z 1,522 after automatic tuning procedure)
Mass Accuracy - MS Mode	< 1 ppm (measured at m/z 609.2807 using an internal reference mass)
Spectral Acquisition Rate - MS Mode	30 spectra/second (spectra acquisition from m/z 100 to 3,200)
Dynamic Range	Up to 5 decades (in-scan dynamic range on co-eluting components)
Mass Range	m/z 25 to 20,000
Polarity Switching	< 1 sec (pos/neg switching)
Mass Accuracy Temperature Stability - MS Mode	Maintain 2 ppm mass accuracy within 2°C drift per hour (temperature: 15°C to 35°C (59°F to 95°F) at constant temperature while setting autotune at 25°C)
Single Point of Control	Single point data system method capability with full control for HPLC system and TOF, stopping sample injection if errors or leaks occur
Ionization sources	Electrospray (ESI), Atmospheric pressure chemical ionization (APCI), Atmospheric pressure photoionization (APPI), Multimode source (ESI and APCI), HPLC-Chip/MS interface, AP/MALDI PDF+ source
Transparent Introduction of Reference Masses	Dual-sprayer orthogonal ESI source, built-in calibrant delivery system, real-time internal reference mass correction for MS operation and heated sources
Autotune	Automated optimization of ion optics and full TOF MS mass axis calibration
Data Mining Tools	One-button extraction of compound-specific spectral and chromatographic information using find compounds algorithms
Molecular Profiling Tools	Measurement of molecular features from LC/MS analyses (MassHunter), differential expression analysis with optional MassHunter Mass Profiler
Sound Emission	< 70 db
Dimensions (L x W x H)	32.7 x 28.7 x 52.4 in. (83 x 73 x 133 cm)
Weight	260 lb. (117.9 kg)
Electrical Requirements	200-220VAC, 50/60Hz, 15A

Click Here to View Specifications for the Agilent 1260 Infinity I LC System

DAD Detector	Specification
Detection Type	1024-element photodiode array
Light Source	Deuterium and tungsten lamps
Wavelength Range	190 - 640 nm
Short Term Noise (ASTM) Single and Multi-Wavelength	± 3 × 10 ^-6 AU at 230 nm/4 nm
Drift	< 0.5 × 10 ^-3 AU/hr at 230 nm
Linear Absorbance Range	> 2.0 AU (5%) at 265 nm
Wavelength Accuracy	± 1 nm
Wavelength Bunching	1 - 400 nm
Slit Width	4 nm
Diode Width	~ 0.5 nm
Flow Cell	Standard: 13 µL volume, 10 mm cell path length and 120 bar (1740 psi) pressure maximum
Binary Pump	Specification
Hydraulic System	Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, power transmission by gears and ball screws, floating pistons
Setable Flow Range	0.001 – 5 ml/min, in 0.001 ml/min increments
Flow Range	0.5 - 5.0 mL/min
Flow Precision	≤ 0.07% RSD or ≤ 0.02 min SD, whatever is greater
Flow Accuracy	± 1% or 10 µl/min, what ever is greater
Pressure Operating Range	Operating range 0 to 600 bar (0 – 7800 psi) up to 5 ml/min
Pressure Pulsation	< 2 % amplitude (typically < 1.3 %), or < 0.3 MPa (3 bar), whatever is greater, at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar, 147 psi)
Compressibility Compensation	User-selectable, based on mobile phase compressibility
Recommended pH Range	1.0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless stee
Gradient Formation	High-pressure binary mixing
Delay Volume	600 to 800 µl, dependent on back pressure
Composition Range	0 to 100% (1 to 99% or 5 µl/min per channel, whatever is greater is recommended)
Composition Precision	< 0.15% RSD or < 0.04 min SD whatever is greater
Composition Accuracy	± 0.35% absolute at 2 mL/min, at 10 MPa (100 bar)
Autosampler	Specification
Injection Range	0.1 - 100 µl in 0.1 µl increments
Precision	< 0.25 % RSD of peak areas from 5 to 100 µl < 1 % RSD of peak areas from 1 to 5 µl
Pressure Range	Operating range 0 - 60 MPa (0 - 600 bar, 0 - 8850 psi)
Sample Viscosity Range	0.2 to 50 cp
Sample Capacity	100 × 2 mL vials in 1 tray 40 × 2 mL vials in ½ tray 15 × 6 mL vials in ½ tray (Agilent vials only)
Injection Cycle Time	50 s for draw speed 200 µL/min, ejection speed 200 µL/min, injection volume 5 µL
Carryover	Typically < 0.1 %, < 0.05 % with external needle cleaning
ALS Thermostat	Specification
Temperature Range	4°C to 40°C in 1°C increments
Temperature Accuracy at Ambient Temperatures < 25°C and Humidity < 50%	3°C to +8°C at a setpoint of 4°C
Temperature Accuracy at Ambient Temperatures > 30°C and Humidity > 60%	3°C to +9°C at a setpoint of 4°C
Column Compartment	Specification
Temperature Range	Ambient -10°C to 80°C at flow rates up to 5 mL/min
Temperature Stability	± 0.15 °C
Temperature Accuracy	± 0.8°C (± 0.5°C with calibration)
Column Capacity	Three 30 cm
Warm-Up/Cool-Down Time	5 minutes from ambient to 40°C 10 minutes from 40°C to 20°C
Dead Volume	3 µl left heat exchanger 6 µl right heat exchanger
Degasser	Specification
Number of Solvent Channels	4
Maximum Flow Rate	0 - 5 mL/min per channel
Internal Volume Per Channel	Typically 1 ml per channel
Materials in Contact with Solvent	PTFE, PEEK
pH Range	1 - 14

Computer Specifications
Model	Dell Optiplex 7010 Desktop PC
Processor	Intel Core i5-6500 Processor 3.2GHz
RAM	16GB
Hard Drive	250GB Internal SSD
Operating System	Windows 10 Professional, 64bit