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DAD Detector
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Specification
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Detection Type
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1024-element photodiode array
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Light Source
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Deuterium and tungsten lamps
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Wavelength Range
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190 - 640 nm
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Short Term Noise (ASTM) Single and Multi-Wavelength
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± 3 × 10
-6 AU at 230 nm/4 nm
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Drift
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< 0.5 × 10
-3 AU/hr at 230 nm
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Linear Absorbance Range
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> 2.0 AU (5%) at 265 nm
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Wavelength Accuracy
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± 1 nm
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Wavelength Bunching
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1 - 400 nm
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Slit Width
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4 nm
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Diode Width
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~ 0.5 nm
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Flow Cell
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Standard: 13 µL volume, 10 mm cell path length and 120 bar (1740 psi) pressure maximum
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Binary Pump
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Specification
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Hydraulic System
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Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, power transmission by gears and ball screws, floating pistons
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Setable Flow Range
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0.001 – 5 ml/min, in 0.001 ml/min increments
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Flow Range
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0.5 - 5.0 mL/min
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Flow Precision
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≤ 0.07% RSD or ≤ 0.02 min SD, whatever is greater
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Flow Accuracy
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± 1% or 10 µl/min, what ever is greater
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Pressure Operating Range
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Operating range 0 to 600 bar (0 – 7800 psi) up to 5 ml/min
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Pressure Pulsation
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< 2 % amplitude (typically < 1.3 %), or < 0.3 MPa (3 bar), whatever is greater, at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar, 147 psi)
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Compressibility Compensation
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User-selectable, based on mobile phase compressibility
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Recommended pH Range
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1.0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless stee
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Gradient Formation
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High-pressure binary mixing
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Delay Volume
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600 to 800 µl, dependent on back pressure
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Composition Range
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0 to 100% (1 to 99% or 5 µl/min per channel, whatever is greater is recommended)
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Composition Precision
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< 0.15% RSD or < 0.04 min SD whatever is greater
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Composition Accuracy
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± 0.35% absolute at 2 mL/min, at 10 MPa (100 bar)
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Autosampler
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Specification
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Injection Range
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0.1 - 100 µl in 0.1 µl increments
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Precision
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< 0.25 % RSD of peak areas from 5 to 100 µl
< 1 % RSD of peak areas from 1 to 5 µl
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Pressure Range
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Operating range 0 - 60 MPa (0 - 600 bar, 0 - 8850 psi)
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Sample Viscosity Range
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0.2 to 50 cp
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Sample Capacity
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100 × 2 mL vials in 1 tray
40 × 2 mL vials in ½ tray
15 × 6 mL vials in ½ tray (Agilent vials only)
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Injection Cycle Time
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50 s for draw speed 200 µL/min, ejection speed 200 µL/min, injection volume 5 µL
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Carryover
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Typically < 0.1 %, < 0.05 % with external needle cleaning
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ALS Thermostat
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Specification
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Temperature Range
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4°C to 40°C in 1°C increments
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Temperature Accuracy at Ambient Temperatures < 25°C and Humidity < 50%
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3°C to +8°C at a setpoint of 4°C
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Temperature Accuracy at Ambient Temperatures > 30°C and Humidity > 60%
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3°C to +9°C at a setpoint of 4°C
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Column Compartment
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Specification
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Temperature Range
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Ambient -10°C to 80°C at flow rates up to 5 mL/min
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Temperature Stability
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± 0.15 °C
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Temperature Accuracy
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± 0.8°C (± 0.5°C with calibration)
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Column Capacity
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Three 30 cm
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Warm-Up/Cool-Down Time
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5 minutes from ambient to 40°C
10 minutes from 40°C to 20°C
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Dead Volume
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3 µl left heat exchanger
6 µl right heat exchanger
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Degasser
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Specification
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Number of Solvent Channels
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4
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Maximum Flow Rate
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0 - 5 mL/min per channel
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Internal Volume Per Channel
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Typically 1 ml per channel
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Materials in Contact with Solvent
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PTFE, PEEK
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pH Range
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1 - 14
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